Association of TLR4 Gene Genotype and Resistance Against Salmonella enteritidis Natural Infection in Kampung Chicken
DOI:
https://doi.org/10.3923/ijps.2013.445.450Keywords:
Genotyping, kampung chicken, non-specific immune, TLR4 geneAbstract
Eggs of kampung chicken play an important role as substance in ‘jamu preparation’ in Indonesia, mostly provided and consumed without cooking. Salmonella free eggs become significant in producing the safe ‘jamu preparation’ and such eggs might be produced by chickens which have high resistancy to this bacteria. One of excellent markers showing resistance of chicken against Salmonella is an active Toll-like Receptor 4 (TLR4) gene. TLR4 is a phagocytes cell surface receptor that plays a role to recognize lipopolysaccaride (LPS) of gram negative bacteria including Salmonella enteritidis. It is transcribed by TLR4 gene and conserved in the activation of the non-specific immune system. The aim of the research was to prove how kampung chicken resistant against natural infection of S. enteritidis, using TLR4 gene as marker. TLR4 gene was genotyped in 50 kampung chickens with PCR-RFLP. Then biological assays of resistance indicator were measured. The genotyping result on exon 2 (220 bp in size) identified 3 genotypes of TLR4 gene in kampung chicken: AA, AG and GG. Concentration of leucocytes and their differentiation were not significantly different in AG and GG genotype. The value of it from AA genotype was similar to them. There was no S. enteritidis finding in blood and eggs produced by AA, AG, GG chickens. Specific IgY to S. enteritidis was positively found in kampung chickens serum and egg yolk. Concentration of specific IgY in kampung chicken egg yolk was found very high. The study postulated that most likely kampung chicken resistant to S. enteritidis natural infection.
References
Abcam, 2013. Indirect ELISA protocol. http://www.abcam.com/ps/pdf/protocols/Indirect%20ELISA%20protocol.pdf.
Aditya, N., I.B.N. Swacita and T.P.G. Ketut, 2012. Deteksi bakteri Salmonella spp dan pengujian kualitas telur ayam buras [Detection of Salmonella spp bacteria and native chicken egg quality testing]. Indonesia Med. Vet., 3: 320-329.
Takeda, K. and S. Akira, 2004. TLR signaling pathways. Semin. Immunol., 16: 3-9.
Beaumont, C., J. Protais, F. Pitel, G. Leveque and D. Malo et al., 2003. Effect of two candidate genes on the Salmonella carrier state in fowl. Poult. Sci., 82: 721-726.
Bell, D.D. and W.J. Weaver, 2002. Commercial Chicken Meat and Egg Production. 5th Edn., Kluwer Academic Publishers, USA.
Bhunia, A.K., 2008. Foodborne Microbial Pathogens: Mechanisms and Pathogenesis. Springer, New York, USA., ISBN-13: 9780387745374, Pages: 276.
Calenge, F., P. Kaiser, A. Vignal and C. Beaumont, 2010. Genetic control of resistance to salmonellosis and to Salmonella carrier-state in fowl: A review. Genet. Sel. Evol., Vol. 42.
Ermertcan, A.T., F. Ozturk and K. Gunduz, 2011. Toll-like receptors and skin. J. Eur. Acad. Dermatol. Venereol., 25: 997-1006.
Gantois, I., R. Ducatelle, F. Pasmans, Haesebrouck, R. Gast, T.J. Humpre and V. Immerseel, 2009. Mechanisms of Egg Contamination. In: Federation of European Microbiological Societies, Enteritidis, S. (Ed.). Blackwell Publishing, Belgium.
Jain, N.C., 1993. Essentials of Veterinary Hematology. Lea and Febiger, Philadelphia, PA., USA., ISBN-13: 9780812114379, Pages: 417.
Kabelitz, D., 2007. Expression and function of Toll-like receptors in T lymphocytes. Curr. Opin. Immunol., 19: 39-45.
Li, H.F., Y. Hu, H. Hu, C. Song and J.T. Shu et al., 2013. Genetic differ in TLR4 gene polymorphisms and expression involved in Salmonella natural and artificial infection respectively in Chinese native chicken breeds. Mol. Biol. Rep., 40: 5005-5012.
Lorenz, E., J.P. Mira, K.L. Frees and D.A. Schwartz, 2002. Relevance of mutations in the TLR4 receptor in patients with gram-negative septic shock. Arch. Intern. Med., 162: 1028-1032.
Nataamijaya, A.G., 2010. Pengembangan potensi ayam lokal untuk menunjang peningkatan kesejahteraan petani [The development potential of local chickens to support the promotion of the welfare of farmers]. J. Litbang Pertanian, 29: 131-138.
Nei, M., 1987. Molecular Evolutionary Geneticcs. Columbia University Press, New York, ISBN: 9780231063210, Pages: 512.
Sambrook, J., E.F. Fritsh and T. Maniatis, 1989. Molecular Cloning: A Laboratory Manual. Cold Harbor Laboratory Press, New York, USA.
Schade, R. and A. Hlinak, 1996. Egg yolk antibodies, state of the art and future prospects. Altex, 13: 5-9.
Swenson, M.J., 1984. Physiological Properties and Cellular and Chemical Constituents of Blood. In: Dukes Physiology of Domestic Animals, Swenson, M.J. (Ed.). 10th Edn., Cornell University Press, London.
Velge, P., A. Cloeckaert and P. Barrow, 2005. Emergence of Salmonella epidemics: The problems related to Salmonella enterica serotyp Enteritidis and multiple antibiotic resistance in other major serotypes. Vet. Res., 36: 267-288.
Gou, Z., R. Liu, G. Zhao, M. Zheng and P. Li et al., 2012. Epigenetic modification of TLRs in leukocytes is associated with increased susceptibility to Salmonella enteritidis in chickens. PLoS ONE, Vol. 7.
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