Polymorphism of the T4842G Myostatin Gene is Associated with Carcass Characteristics in Indonesian Chickens
DOI:
https://doi.org/10.3923/ijps.2016.316.324Keywords:
Carcass characteristics, indonesian chickens, Myostatin gene, polymorphismAbstract
Myostatin, or growth and differentiation factor-8 (GDF-8), is a member of the Transforming Growth Factor (TGF)-β superfamily. This family functions as a negative regulator of skeletal muscle. Mutations in exon 2 have been reported to convert Thymine into Guanine (T4842G) that alters the amino acid leucine into arginine, which is associated with body weight in chickens. The objectives of this study were to identify the polymorphism of T4842G mutation in the myostatin gene in Indonesian chickens and evaluate their effects on carcass characteristics. The gene polymorphism was identified with the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method using the BsrI restriction enzyme. The effect of genotype on carcass and meat quality was analyzed using the SAS General Linear Model (GLM) procedure. Genotyping was performed on 332 chickens from 7 Indonesian chicken populations (Kampung, Merawang, Sentul, Cobb broiler, F1 crossbreed of Kampung x layer, F1 crossbreed of Kampung x Cobb broiler and F2 crossbreed of Kampung x Cobb broiler). The product of amplification was 247 bp. The myostatin|BsrI locus was polymorphic in all populations, producing two alleles (G and T) and three genotypes (GG, GT, TT). Results from the analysis of the allele and genotype frequency showed that the T allele had a higher frequency than the G allele in all populations, except for the F1 crossbreed of the Kampung x Cobb broiler chicken population, which had equal allele frequencies. A significant effect was found between genotype and carcass characteristics in the F2 crossbreed Kampung x Cobb broiler chickens. A SNP in the coding region of myostatin in exon 2 was associated with live weight, carcass weight, breast weight, thighs weight, drum sticks weight, wings weight, breast muscle weight, thighs muscle weight, drum sticks muscle weight and free water. Here, the association of myostatin|BsrI gene polymorphism with chicken carcass characteristics in Indonesian chickens has been demonstrated, providing evidence that myostatin might be an important candidate gene for chicken carcass characteristics.
References
Allendorf, F.W., G. Luikart and S.N. Aitken, 2013. Conservation and the Genetics of Populations. 2nd Edn., Wiley-Blackwell Publishing, UK., ISBN-13: 978-0470671450, Pages: 624.
Baldwin, K.M., F. Haddad, C.E. Pandorf, R.R. Roy and V.R. Edgerton, 2013. Alterations in muscle mass and contractile phenotype in response to unloading models: Role of transcriptional/pretranslational mechanisms. Front. Physiol., Vol. 4.
Barbut, S., A.A. Sosnicki, S.M. Lonergan, T. Knapp and D.C. Ciobanu et al., 2008. Progress in reducing the Pale, Soft and Exudative (PSE) problem in pork and poultry meat. Meat Sci., 79: 46-63.
Baron, E.E., A.A. Wenceslau, L.E. Alvares, K. Nones and D.C. Ruy et al., 2002. High level of polymorphism in the Myostatin chicken gene. Proceedings of the 7th World Congress on Genetics Applied to Livestock Production, August 19-23, 2002, Montpellier, France, pp: 19-23.
Bhattacharya, T.K., R.N. Chatterjee, K. Dushyanth and R. Shukla, 2015. Cloning, characterization and expression of myostatin (growth differentiating factor-8) gene in broiler and layer chicken (Gallus gallus). Mol. Biol. Rep., 42: 319-327.
Bhattacharya, T.K. and R.N. Chatterjee, 2013. Polymorphism of the myostatin gene and its association with growth traits in chicken. Poult. Sci., 92: 910-915.
Burks, T.N. and R.D. Cohn, 2011. Role of TGF-β signaling in inherited and acquired myopathies. Skeletal Muscle, Vol. 1.
Dayton, W.R. and M.E. White, 2008. Cellular and molecular regulation of muscle growth and development in meat animals. J. Anim. Sci., 86: E217-E225.
Duclos, M.J., C. Berri and E. Le Bihan-Duval, 2007. Muscle growth and meat quality. J. Applied Poult. Res., 16: 107-112.
Zhang, G., G. Dai, J. Wang, Y. Wei and F. Ding et al., 2012. Polymorphisms in 5'-upstream region of the myostatin gene in four chicken breeds and its relationship with growth traits in the Bian chicken. Afr. J. Biotechnol., 11: 9677-9682.
Honikel, K.O., 2004. Water-Holding Capacity of Meat. In: Muscle Development of Livestock Animals: Physiology, Genetics and Meat Quality, Pas, M.F., M.E. Te Everts and H.P. Haagsman (Eds.). CABI Publishing, Cambridge, pp: 389-400.
Huff-Lonergan, E. and S.M. Lonergan, 2005. Mechanisms of water-holding capacity of meat: The role of postmortem biochemical and structural changes. Meat Sci., 71: 194-204.
Kambadur, R., A. Bishop, M.S. Salerno, S. McCroskery and M. Sharma, 2004. Role of Myostatin in Muscle Growth. In: Muscle Development of Livestock Animals, Pas, M.F., M.E. Te Everts and H.P. Haagsman (Eds.). CAB International, UK.
Kim, Y.S., N.K. Bobbili, K.S. Paek and H.J. Jin, 2006. Production of a monoclonal anti-myostatin antibody and the effects of in ovo administration of the antibody on posthatch broiler growth and muscle mass. Poult. Sci., 85: 1062-1071.
Kim, Y.S., N.K. Bobbili, Y.K. Lee, H.J. Jin and M.A. Dunn, 2007. Production of a polyclonal anti-myostatin antibody and the effects of in ovo administration of the antibody on posthatch broiler growth and muscle mass. Poult. Sci., 86: 1196-1205.
Kubota, K., F. Sato, S. Aramaki, T. Soh, N. Yamauchi and M.A. Hattori, 2007. Ubiquitous expression of myostatin in chicken embryonic tissues: Its high expression in testis and ovary. Comp. Biochem. Physiol. Part A: Mol. Integr. Physiol., 148: 550-555.
Kumar, S.T.B., N. Dilbaghi, S.P.S. Ahlawat, B. Mishra, M.S. Tantia and R.K. Vijh, 2007. Genetic Relationship among chicken populations of india based on SNP markers of myostatin gene (GDF 8). Int. J. Poult. Sci., 6: 684-688.
Lawrie, R.A. and D.A. Ledward, 2006. Lawrie's Meat Science. 7th Edn., Woodhead Publishing Limited, Cambridge, UK., ISBN-13: 9781845691592, Pages: 442.
Lee, S.J. and A.C. McPherron, 2001. Regulation of myostatin activity and muscle growth. Proc. Natl. Acad. Sci. USA., 98: 9306-9311.
Manceau, M., J. Gros, K. Savage, V. Thome, A. McPherron, B. Paterson and C. Marcelle, 2008. Myostatin promotes the terminal differentiation of embryonic muscle progenitors. Genes Dev., 22: 668-681.
McCroskery, S., M. Thomas, L. Maxwell, M. Sharma and R. Kambadur, 2003. Myostatin negatively regulates satellite cell activation and self-renewal. J. Cell Biol., 162: 1135-1147.
McFarland, D.C., S.G. Velleman, J.E. Pesall and C. Liu, 2007. The role of myostatin in chicken (Gallus domesticus) myogenic satellite cell proliferation and differentiation. Gen. Comp. Endocrinol., 151: 351-357.
Mott, I. and R. Ivarie, 2002. Expression of myostatin is not altered in lines of poultry exhibiting myofiber hyper- and hypoplasia. Poult. Sci., 81: 799-804.
Nei, M. and S. Kumar, 2000. Molecular Evolution and Phylogenetics. Oxford University Press, United Kingdom, ISBN-13: 9780195350517, Pages: 352.
Paswan, C., T.K. Bhattacharya, C.S. Nagaraj, R.N. Chaterjee and M.R. Jayashankar, 2014. SNPs in minimal promoter of myostatin (GDF-8) gene and its association with body weight in broiler chicken. J. Applied Anim. Res., 42: 304-309.
Petracci, M. and C. Cavani, 2011. Muscle growth and poultry meat quality issues. Nutrients, 4: 1-12.
Sambrook, J. and D.W. Russell, 2001. Molecular Cloning: A Laboratory Manual. 3rd Edn., Cold Spring Harbor Laboratory Press, New York, USA., ISBN-13: 9780879695774, Pages: 2344.
SAS., 2008. SAS/STAT® 9.2 User's Guide The GLM Procedure. SAS Institute Inc., Cary, NC., USA.
Scheuermann, G.N., S.F. Bilgili, S. Tuzun and D.R. Mulvaney, 2004. Comparison of chicken genotypes: Myofiber number in pectoralis muscle and myostatin ontogeny. Poult. Sci., 83: 1404-1412.
Sundaresan, N.R., V.K. Saxena, R. Singh, P. Jain and K.P. Singh et al., 2008. Expression profile of myostatin mRNA during the embryonic organogenesis of domestic chicken (Gallus gallus domesticus). Res. Vet. Sci., 85: 86-91.
Thomas, M., B. Langley, C. Berry, M. Sharma, S. Kirk, J. Bass and R. Kambadur, 2000. Myostatin, a negative regulator of muscle growth, functions by inhibiting myoblast proliferation. J. Biol. Chem., 275: 40235-40243.
Ye, X., S.R. Brown, K. Nones, L.L. Coutinho, J.C.M. Dekkers and S.J. Lamont, 2007. Associations of myostatin gene polymorphisms with performance and mortality traits in broiler chickens. Genet. Sel. Evol., 39: 73-89.
Zandi, S., P. Zamani and K. Mardani, 2013. Myostatin gene polymorphism and its association with production traits in western Azerbaijan native chickens. Iran. J. Applied Anim. Sci., 3: 611-615.
Zhang, G.X., X.H. Zhao, J.Y. Wang, F.X. Ding and L. Zhang, 2012. Effect of an exon 1 mutation in the myostatin gene on the growth traits of the Bian chicken. Anim. Genet., 43: 458-459.
Gu, Z., D. Zhu, N. Li, H. Li, X. Deng and C. Wu, 2004. The single nucleotide polymorphisms of the chicken myostatin gene are associated with skeletal muscle and adipose growth. Sci. China Ser. C: Life Sci., 47: 25-30.
Zhu, J., Y. Li, W. Shen, C. Qiao and F. Ambrosio et al., 2007. Relationships between Transforming growth factor-β1, myostatin and decorin: Implications for skeletal muscle fibrosis. J. Biol. Chem., 282: 25852-25863.
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