Evaluation of a Fermentation Source of 25-hydroxycholecalciferol in Broiler Diets
DOI:
https://doi.org/10.3923/ijps.2011.295.299Keywords:
25-hydroxycholecalciferol, bone strength, broilers, fermentation productAbstract
After a seven day depletion period of vitamin D supplementation beginning on day of hatch, male chicks of a commercial broiler strain were placed on diets supplemented with either a commercial source of 25-hydroxycholecalciferol (25-OH-D3) or a new source derived from fermentation. Levels of 0, 2.5, 5, 10, 20, 40, 60 and 80 μg/kg of each source were added to a common basal diet that was considered as marginal in calcium and phosphorus content. Each diet was fed to six pens of five birds each. Birds were then grown to 21 d of age at which time body weight and feed consumption were determined. All birds were euthanized by CO2 inhalation and all toes were removed and ashed. The right tibia was subjected to bone ash determination while the left tibia was subjected to break force analysis. Analysis of the data indicated no significant differences in performance between chicks fed the two sources of 25-OH-D3 (p<0.05) although numerical differences in weight gain and feed conversion were observed that neared statistical significance (p = 0.06 and 0.08, respectively). Estimates of the amount of 25-OH-D3 needed by the bird were approximately 10 μg/kg for tibia ash and 20 μg/kg for body weight and bone breaking force.
References
AOAC, 1990. Official Methods of Analysis. 15th Edn., Association of Official Analytical Chemists, Washington, DC., USA., pp: 200-210.
Bethke, R.M., D.C. Kennard, C.H. Kick and G. Zinzalian, 1929. The calcium-phosphorus relationship in the nutrition of the growing chick. Poult. Sci., 8: 257-265.
Cantor, A.H. and W.L. Bacon, 1978. Performance of caged broilers fed vitamin D3 and 25-hydroxy vitamin D3. Poult. Sci., 57: 1123-1123.
Collins, E.D. and A.W. Norman, 1991. Vitamin D. In: Handbook of Vitamins. Machlin, L.J. (Ed.). Marcel Dekker, New York, USA., pp: 59-98.
Edwards, Jr. H.M., R.B. Shirley, W.B. Escoe and G.M. Pesti, 2002. Quantitative evaluation of 1-alpha-hydroxycholecalciferol as a cholecalciferol substitute for broilers. Poult. Sci., 81: 664-669.
Fritts, C.A. and P.W. Waldroup, 2003. Effect of source and level of vitamin D on live performance and bone development in growing broilers. Applied Poult. Res., 12: 45-52.
Fritts, C.A., G.F. Erf, T.K. Bersi and P.W. Waldroup, 2004. Effect of source and level of vitamin D on immune function in growing broilers. J. Appl. Poult. Res., 13: 263-273.
Hart, E.B., H.T. Scott and O.L. Kline, 1930. The calcium-phosphorus ratio in the nutrition of growing chicks. Poult. Sci., 9: 296-306.
McNaughton, J.L., E.J. Day and B.C. Dilworth, 1977. The chick’s requirement for 25-hydroxycholecalciferol and cholecalciferol. Poult. Sci., 56: 511-516.
Mitchell, R.D., H.M. Edwards Jr. and G.R. McDaniel, 1997. The effects of ultraviolet light and cholecalciferol and its metabolites on the development of leg abnormalities in chickens genetically selected for high and low incidence for tibial dyschondroplasia. Poult. Sci., 76: 346-354.
Robbins, K.R., 1986. A Method, SAS Program and Examples for Fitting the Broken-Line to Growth Data. University of Tennessee Agricultural Experiment station Research Report, University of Tennessee, Knoxville, TN.
Robbins, K.R., H.W. Norton and D.H. Baker, 1979. Estimation of nutrient requirements from growth data. J. Nutr., 109: 1710-1714.
SAS, 1991. SAS User’s Guide: Statistics. Version 6.03 (Edn.), SAS Institute Inc., Cary, NC..
Soares, J.H., M.R. Swerdel and E.H. Bossard, 1978. Phosphorus availability: 1. The effect of chick age and vitamin D metabolites on the availability of phosphorus in Defluorinated phosphate. Poult. Sci., 57: 1305-1312.
Sunde, M.L., 1975. What about 25-Hydroxycholecalciferol for poultry. Proc. Dist. Res. Council, 30: 53-62.
Waldroup, P.W., C.B. Ammerman and R.H. Harms, 1963. The relationship of phosphorus, calcium and vitamin D3 in the diet of broiler-type chicks. Poult. Sci., 42: 982-989.
Waldroup, P.W., J.H. Kersey, E.A. Saleh, C.A. Fritts and F. Yan et al., 2000. Nonphytate phosphorus requirement and phosphorus excretion of broiler chicks fed diets composed of normal or high available phosphate corn with and without microbial phytase. Poult. Sci., 79: 1451-1459.
Waldroup, P.W., J.E. Stearns, C.B. Ammerman and R.H. Harms, 1965. Studies on the vitamin D3 requirement of the broiler chick. Poult. Sci., 44: 543-548.
Ward, N.E., 1995. Research examines use of 25-OH vitamin D3 in broiler diets. Feedstuffs, 67: 12-15.
Wilgus, H.S. Jr., 1931. The quantitative requirements of the growing chick for calcium and phosphorus. Poult. Sci., 10: 107-117.
Yan, F., C.A. Keen, K.Y. Zhang and P.W. Waldroup, 2005. Comparison of methods to evaluate bone mineralization. J. Applied Poult., 14: 492-498.
Yan, F. and P.W. Waldroup, 2006. Nonphytate phosphorus requirement and phosphorus excretion of broiler chicks fed diets composed of normal or high available phosphate corn as influenced by phytase supplementation and vitamin D source. Int. J. Poult. Sci., 5: 219-228.
Yang, H.S., P.E. Waibel and J. Brenes, 1973. Evaluation of vitamin D supplements by biological assay using the turkey. J. Nutr., 103: 1187-1194.
Yarger, J.G., C.A. Saunders, J.L. McNaughton, C.L. Quarles, B.W. Hollis and R.W. Gray, 1995. Comparison of dietary 25-hydroxycholecalciferol and cholecalciferol in broiler chickens. Poult. Sci., 74: 1159-1167.
Yu, S. and J.G. Morris, 1999. Chloride requirement of kittens for grown is less than current recommendations. J. Nutr., 129: 1909-1914.
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