Litter Treatment with Aluminum Sulfate (Alum) Produced an Inconsistent Reduction in Horizontal Transmission of Campylobacter in Chickens
DOI:
https://doi.org/10.3923/ijps.2017.31.36Keywords:
Alum, Campylobacter jejuni, foodborne illness, horizontal transmission, poultry litterAbstract
Background and Objective: Campylobacteriosis is a significant health problem worldwide and poultry are considered as one of the main vehicles of transmission. This study was conducted to determine whether alum reduces Campylobacter colonization in broilers by reducing horizontal transmission between birds or by reducing Campylobacter counts in birds already colonized (Therapeutic efficacy). Materials and Methods: Two replicate experiments were conducted and in each experiment, day of hatch broiler chicks (n = 295) were divided into 7 treatment groups including controls. Each treatment was reared in either no (0 kg), low (0.78 kg m–2) or high (1.58 kg m–2) concentrations of aluminum sulfate (alum; Al+ Clear). During days 7, 14, 28 and 42, ten birds from each treatment were analyzed for Campylobacter counts in the ceca. To evaluate whether alum inhibits horizontal transmission between birds, Campylobacter negative birds were reared with seeder birds that served as carriers. Results: Alum reduced (p<0.05) horizontal transmission of Campylobacter at 14 and 28 days in experiment 1 and only with the highest concentration of alum at 42 days in experiment 2. To evaluate the therapeutic efficacy of alum, all birds were inoculated with Campylobacter (5.2×106 CFU mL–1) prior to placement in pens. Infected birds reared on low or high alum had lower (p<0.05) Campylobacter counts at 14 and 28 days in only 1 of 2 experiments. At 42 days, there were no differences in cecal Campylobacter counts between alum treated and untreated controls in experiment 1 and for only the highest concentration in experiment 2. Conclusion: It appears treating litter with alum is not a consistent way to reduce enteric Campylobacter counts.
References
WHO., 2011. Campylobacter. Fact Sheet, World Health Organization, Geneva, Switzerland. http://www.who.int/mediacentre/factsheets/fs255/en/.
CDC., 2015. 2015 food safety report. Centers for Disease Control and Prevention. https://www.cdc.gov/foodnet/pdfs/foodnet-mmwr-progress-508-final.pdf.
Wagenaar, J.A., D.G. Newell, R.S. Kalupahana and L. Mughini-Gras, 2015. Campylobacter Animal Reservoirs, Human Infections and Options for Control. In: Zoonoses-Infections Affecting Humans and Animals, Sing, A. (Ed.). Springer, Netherlands, pp: 159-177.
Robyn, J., G. Rasschaert, F. Pasmans and M. Heyndrickx, 2015. Thermotolerant Campylobacter during broiler rearing: Risk factors and intervention. Comp. Rev. Food Sci. Food Saf., 14: 81-105.
Vandeplas, S., C. Marcq, R.D. Dauphin, Y. Beckers, P. Thonart and A. Thewis, 2008. Contamination of poultry flocks by the human pathogen Campylobacter spp. and strategies to reduce its prevalence at the farm level. Biotechnol. Agron. Soc. Environ., 12: 317-334.
Newell, D.G., K.T. Elvers, D. Dopfer, I. Hansson and P. Jones et al., 2011. Biosecurity-based interventions and strategies to reduce Campylobacter spp. on poultry farms. Appl. Environ. Microbiol., 77: 8605-8614.
WHO., 2013. The global view of campylobacteriosis: Report of an expert consultation, Utrecht, Netherlands, 9-11 July 2012. World Health Organization, Geneva. http://apps.who.int/iris/handle/10665/80751.
Epps, S.V.R., R.B. Harvey, M.E. Hume, T.D. Phillips, R.C. Anderson and D.J. Nisbet, 2013. Foodborne Campylobacter, Infections, metabolism, pathogenesis and reservoirs. Int. J. Environ. Res. Public Health, 10: 6292-6304.
Line, J.E., 2002. Campylobacter and Salmonella populations associated with chickens raised on acidified litter. Poult. Sci., 81: 1473-1477.
Moore, P.A. and D.R. Edwards, 2007. Long-term effects of poultry litter, alum-treated litter, and ammonium nitrate on phosphorus availability in soils. J. Environ. Qual., 36: 163-174.
Seiter, J.M., K.E. Staata-Borda, M. Ginder-Vogel and D.L. Sparks, 2008. XANES spectroscopic analysis of phosphorus speciation in alum-amended poultry litter. J. Eniron. Qual., 37: 477-485.
Guo, M. and W. Song, 2009. Nutrient value of alum-treated poultry litter for land application. Poult. Sci., 88: 1782-1792.
Choi, I.H. and P.A. Moore Junior, 2008. Effects of liquid aluminum chloride additions to poultry litter on broiler performance, ammonia emissions, soluble phosphorus, total volatile fatty acids and nitrogen contents of litter. Poult. Sci., 87: 1955-1963.
Zhang, H.F., H.C. Jiao, Z.G. Song and H. Lin, 2011. Effect of alum-amended litter and stocking density on ammonia release and footpad and hock dermatitis of broilers. Agric. Sci. China, 10: 777-785.
Line, J.E. and J.S. Bailey, 2006. Effect of on-farm litter acidification treatments on Campylobacter and Salmonella populations in commercial broiler houses in Northeast Georgia. Poult. Sci., 85: 1529-1534.
Choi, I.H., J.N. Kim and Y.M. Kwon, 2008. Effects of chemical treatments on pH and bacterial population in poultry litter: A laboratory experiment. Br. Poult. Sci., 49: 497-501.
Rothrock, M.J.Jr., K.L. Cook, J.G. Warren and K. Sistani, 2008. The effect of alum addition on microbial communities in poultry litter. Poult. Sci., 87: 1493-1503.
Chen, Z. and X. Jiang, 2014. Microbiological safety of chicken litter or chicken litter-based organic fertilizers: A review. Agriculture, 4: 1-29.
Sahin, O., I.I. Kassem, Z. Shen, J. Lin, G. Rajashekara and Q. Zhang, 2015. Campylobacter in poultry: Ecology and potential interventions. Avian Dis., 59: 185-200.
Cole, K., M.B. Farnell, A.M. Donoghue, N.J. Stern and E.A. Svetoch et al., 2006. Bacteriocins reduce Campylobacter colonization and alter gut morphology in Turkey poults. Poult. Sci., 85: 1570-1575.
Perveen, F. and Z. Hussain, 2012. Use of statistical techniques in analysis of biological data. Basic Res. J. Agric. Sci. Rev., 1: 1-10.
SAS., 2011. Base SAS® 9.3 Procedures Guide. SAS Institute Inc., Cary, NC., USA.
Shaheen, B.W., M.E. Miller and O.A. Oyarzabal, 2007. In vitro survival at low pH and acid adaptation response of Campylobacter jejuni and Campylobacter coli. J. Food Saf., 27: 326-343.
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