A Study on Biochemical Composition of the Inner Wall of the Oocyst of Eimeria necatrix


Authors

  • G. Patra Department of Veterinary Parasitology, College of Veterinary Sciences and A.H., CAU, Selesih, Aizawl, Mizoram, India
  • T.K. Rajkhowa Department of Veterinary Pathology, College of Veterinary Sciences and A.H., CAU, Selesih, Aizawl, Mizoram, India
  • Lalhruaitluangi Sailo Department of Veterinary Public Health, College of Veterinary Sciences and A.H., CAU, Selesih, Aizawl, Mizoram, India
  • W. Ramdas Singh Department of Pharmacology and Toxicology, College of Veterinary Sciences and A.H., CAU, Selesih, Aizawl, Mizoram, India
  • Gunjan Das Department of Veterinary Medicine, College of Veterinary Sciences and A.H., CAU, Selesih, Aizawl, Mizoram, India
  • Kh. Victoria Chanu Department of Veterinary Biochemistry College of Veterinary Sciences and A.H., CAU, Selesih, Aizawl, Mizoram, India
  • M. Ayub Ali Department of Veterinary Biochemistry College of Veterinary Sciences and A.H., CAU, Selesih, Aizawl, Mizoram, India

DOI:

https://doi.org/10.3923/ijps.2009.1214.1217

Keywords:

Alanine aminotransferase, aspartate aminotransferase, Eimeria necatrix, electrolyte composition, Oocyst

Abstract

A biochemical study was made to elucidate the biochemical composition of the inner wall of oocyst of Eimeria necatrix and then compared with cellular component of the oocyst. In addition to total protein, glucose and cholesterol, several electrolyte components have been estimated in the present study. The investigation also revealed high activity of Alanine and Asparate aminotransferases in the inner wall of the oocyst of Eimeria necatrix. It seems that the biochemical composition and the enzymes in the inner wall of the oocyst of Eimeria necatrix are of significant value for better metabolic processes in Eimeria spp. as well as for better understanding of possible drug-parasite interaction.

References

Ahmedov, E.I., F.Z. Mamedova and S.M. Mamedova, 2006. Pathogenis of eimeriosis in the local chicken breeds (Apicomplexa, Coccidia, E. tenella). Trans. Inst. Zool. Baku, 28: 170-175.

Aliyeva, F.Z., 1999. Effect of amino acid derivatives on the pathogenesis of the chicken eimeriosis. Azerbaizan Vet. J., 1: 62-66.

Belli, S.I., N.C. Smith and D.J.P. Ferguson, 2006. The coccidian oocyst: A tough nut to crack! Trends Parasitol., 22: 416-423.

Dubey, J.P., N.L. Miller and J.K. Frenkel, 1970. The Toxoplasma gondii oocyst from cat faeces. J. Exp. Med., 132: 636-662.

Mai, K., A.P. Sharman, A.R. Walker, M. Katrib and D. de Souza et al., 2009. Oocyst wall formation and composition in coccidian parasites. Mem. Inst. Oskaldo Cruz, 104: 281-289.

Kruszynski, J., 1951. A microchemical study of Plasmodium gallinaceum by micro incineration. Ann. Trop. Med. Parasitol., 45: 85-91.

Monne, L. and G. Honig, 1954. On the properties of the shells of Coccidian oocysts. Arkiv Zool., 7: 251-256.

Nyberg, P.A., D.H. Bauer and S.E. Knapp, 1968. Carbon dioxide as the initial stimulus for excystation of Eimeria tenella oocyst. J. Eukaryotic Protozool., 15: 144-148.

Nyberg, P.A. and S.E. Knapp, 1970. Effect of sodium hypochlorite on the oocyst wall of Eimeria tenella as shown by electron microscopy. Proc. Helminthol. Soc. Wash, 37: 32-36.

Ryley, J.F., 1973. Cytochemistry, Physiology and Biochemistry. In: The Coccidia: Eimeria, Isospora, Toxoplasma and Related Genera, Hammond, D.H. and P.L. Long (Eds.). University Park Press, Baltimore, London, pp: 145-181.

Schares, G., N. Pantchev, D. Barutzki, H. Ao, C. Bauer and F.J. Conraths, 2005. Oocyst of Neospora caninum, Hammondia heydorni, Toxoplasma gondii and Hammondia hammondi in faeces collected from dogs in Germany. Int. J. Parasitol., 35: 1525-1537.

Shirley, M.W., 1995. Eimeria Species and Strains of Chickens. In: Biotechnology-Guidelines on Techniques in Coccidiosis Research, Eckert, J., R. Braun, M.W. Shirley and P. Coudert (Eds.). European Commision, Luxemburg, pp: 1-51.

Stotish, R.L., C.C. Wang and M. Meyenhofer, 1978. Structure and composition of the oocyst wall of Eimeria tenella. J. Parasitol., 64: 1074-1081.

Visco, R.J., 1975. Eimeria tenella in gnotobiotic chickens, Haematocrit, weight change, caecal pathology and mortality. Parasitology, 61: 194-198.

Williams, R.B., 1999. Three enzymes newly identified from the genus eimeria and two more newly identified from E. maxima, leading to the discovery of some alipnatic acids with activity against coccidian of the domesticated fowl. Vet. Res. Commun., 23: 151-163.

Yolchiyev, Y.Y.O. and F.Z.G. Mamedova, 2007. Glutamate dehydrogenase, alamine and asparate aminotransferase of Eimeria tenella (apicomplexa, coccidia) oocysts and effect of aminoacids`a derivatives on their activities. Int. J. Nat. Eng. Sci., 1: 23-27.

Downloads

Published

2009-11-15

Issue

Section

Research Article

How to Cite

Patra, G., Rajkhowa, T., Sailo, L., Singh, W. R., Das, G., Chanu, K. V., & Ali, M. A. (2009). A Study on Biochemical Composition of the Inner Wall of the Oocyst of Eimeria necatrix. International Journal of Poultry Science, 8(12), 1214–1217. https://doi.org/10.3923/ijps.2009.1214.1217

Most read articles by the same author(s)