PCR Based Diagnosis of Eimeria tenella Infection in Broiler Chicken


Authors

  • Gautam Patra Department of Veterinary Parasitology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram, India
  • M. Ayub Ali Department of Veterinary Physiology and Biochemistry, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram, India
  • Kh. Victoria Chanu Department of Veterinary Physiology and Biochemistry, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram, India
  • L. Jonathan SRF-RCRL, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram, India
  • L.K. Joy SRF-RCRL, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram, India
  • M. Prava Department of Veterinary Physiology and Biochemistry, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram, India
  • R. Ravindran Department of Veterinary Pathology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram, India
  • G. Das Department of Veterinary Clinical Medicine, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl, Mizoram, India
  • L. Inaotombi Devi Department of MLT, RIPANS, Aizawl, Mizoram, India

DOI:

https://doi.org/10.3923/ijps.2010.813.818

Keywords:

Chicken coccidiosis, haematology, histopathology, molecular diagnosis, serum biochemistry

Abstract

The present investigation was undertaken to evaluate histo-pathological, haemotological and biochemical changes in broiler chicken naturally infected with Eimeria tenella. A Polymerase Chain Reaction (PCR) based assay was also done for the detection, identification and differentiation of pathogenic species of Eimeria in poultry. Post mortem examination revealed petechial haemorrhages, oedema, necrosis and sloughing of caecal epithelium. Histopathological evidence showed leakage of blood, oedema and necrosis. Haemotological studies revealed that coccidial infection caused significant decrease in the value of haemoglobin and Packed Cell Volume (PCV). The value of Mean Corpuscular Volume (MCV) and Mean Corpuscular Haemoglobin Concentration (MCHC) revealed that caecal coccidiosis resulted in macrocytic hypochromic anaemia. Biochemical serum analysis of coccidial infected chicken showed a significant increase in level of glucose, Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST) while there was a significant decrease in the level of alkaline phosphatase (SAP) and cholesterol. In PCR using specific primers for E. tenella only the ITS 1 regions of ribosomal DNA (rDNA) could be amplified.

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Published

2010-07-15

Issue

Vol. 9 No. 8 (2010)

Section

Research Article

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Copyright (c) 2010 Asian Network for Scientific Information

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This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

How to Cite

Patra, G., Ali, M. A., Chanu, K. V., Jonathan, L., Joy, L., Prava, M., Ravindran, R., Das, G., & Devi, L. I. (2010). PCR Based Diagnosis of Eimeria tenella Infection in Broiler Chicken. International Journal of Poultry Science, 9(8), 813–818. https://doi.org/10.3923/ijps.2010.813.818

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