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Monoclonal Antibody EIV-E12 Recognizes a Glycoprotein Antigen, Which Differs from the B-Cell-Specific chB6 (Bu-1) Antigen
Abstract
The antigen (s) recognized by the EIV-E12 monoclonal antibody (mAb) is first detected in the embryonic bursal mesenchyme on the precursor to the bursal secretory dendritic cell. Later in bursal embryonic development the prebursal stem cells which enter the developing follicle express the antigen recognized by mAb EIV-E12. The goal of this project is to characterize the antigen recognized by mAb EIV-E12. In western blotting the deglycosylated bursal proteins showed reduced reactivity to mAb EIV-E12, indicating, that the EIV-E12 epitope of the antigen may consist of a carbohydrate group that is common to chicken B-cells. The EIV-E12 positive antigen is expressed by the plasma cells of terminal maturation of B-cells unlike the B-cell specific chB6 antigen. These differences in the expression patterns of EIV-E12 and chB6 positive antigens are confirmed by their different molecular weights of 200 and 65 kDa, respectively.
Keywords:
Bursal B-cell, monoclonal antibody EIV-E12, novel B-cell antigenArticle Metrics
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Copyright (c) 2015 Asian Network for Scientific Information
This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
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Nuthalapati, N., Alugubelly, N., Felfoldi, B., Bodi, I., Fejszak, N., Pharr , G., & Olah, I. (2015). Monoclonal Antibody EIV-E12 Recognizes a Glycoprotein Antigen, Which Differs from the B-Cell-Specific chB6 (Bu-1) Antigen. International Journal of Poultry Science, 14(8), 479–484. https://doi.org/10.3923/ijps.2015.479.484
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