Research Article Open Access

The Recognition of a vlhA Protein from the F-Strain of Mycoplasma gallisepticum with Monoclonal Antibody 6F10

Authors

CORRESPONDING AUTHOR
G.T. Pharr
Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA
|
S.L. Branton
1JSDA, ARS, South Central Poultry Research Unit, Mississippi state: MS 39762, USA
|
A.M. Cooksey
Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA
|
L.A. Hanson
Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA
|
S.C. Burgess
Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA
|
C.C. Bottoms
Department of Math and Natural Sciences, Collin County Community College, Frisco, TX 75035, USA
|
F.M. McCarthy
Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA
|
S.D. Collier
1JSDA, ARS, South Central Poultry Research Unit, Mississippi state: MS 39762, USA
Published on: July 15, 2006
DOI 10.3923/ijps.2006.789.795
PDF

Abstract

The goal of this project is to identify the genes encoding M. gallisepticum F-strain surface proteins recognized by specific antibody reagents to characterize the individual role of each gene product in host colonization. Here we report the characterization of a 70-kDa surface protein recognized by monoclonal antibody 6F10. The surface protein was gel isolated and digested with trypsin for evaluation with mass spectrometry. The results showed that 19 out of the 21 tryptic peptides matched with proteins of the vlhA lipoprotein family. We suggest that monoclonal antibody 6F10 is specific for the predominantly expressed vlhA protein (formerly pMGA) of the F-strain of M. gallisepticum.

Keywords:

F-strain, mass spectrometry, monoclonal antibody 6F10, Mycoplasma gallisepticum, vlhA

Article Metrics

291 Abstract Views
90 File Views
0 Downloads

License

Copyright (c) 2006 Asian Network for Scientific Information

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

Article Citation Metrics

Find The Recognition of a vlhA Protein from the F-Strain of Mycoplasma gallisepticum with Monoclonal Antibody 6F10 on PlumX

How to Cite

Pharr, G., Branton, S., Cooksey, A., Hanson, L., Burgess, S., Bottoms, C., McCarthy, F., & Collier, S. (2006). The Recognition of a vlhA Protein from the F-Strain of Mycoplasma gallisepticum with Monoclonal Antibody 6F10. International Journal of Poultry Science, 5(8), 789–795. https://doi.org/10.3923/ijps.2006.789.795
Crossref
Scopus
Google Scholar
Europe PMC

Most read articles by the same author(s)

1 2 3 4 > >>