Research Article Open Access

Improved Detection of Campylobacter jejuni, Listeria monocytogenes and Salmonella Typhimurium from Raw Meat Products Using Conventional and Newly Developed TaqMan Assays

Authors

Giang Nguyen
National Institute for Food Control, Ha Noi, Viet Nam,
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Magda Ismail Abo-Samaha
Department of Pathobiology, College of Veterinary Medicine, Nursing and Allied Health, Tuskegee University, Tuskegee, Alabama-36088, USA Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt
|
Gopal Reddy
Department of Pathobiology, College of Veterinary Medicine, Nursing and Allied Health, Tuskegee University, Tuskegee, Alabama-36088, USA
|
Mohammed Abdulrahman
Department of Pathobiology, College of Veterinary Medicine, Nursing and Allied Health, Tuskegee University, Tuskegee, Alabama-36088, USA
|
Temesgen Samuel
Department of Pathobiology, College of Veterinary Medicine, Nursing and Allied Health, Tuskegee University, Tuskegee, Alabama-36088, USA
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Abiodun Adesiyun
Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, Pretoria, South Africa
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CORRESPONDING AUTHOR
Woubit Abdela
Department of Pathobiology, College of Veterinary Medicine, Nursing and Allied Health, Tuskegee University, Tuskegee, Alabama-36088, USA
Published on: June 15, 2015
DOI 10.3923/ijps.2015.364.375
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Abstract

The most frequent bacteria contaminating raw meat samples from local retail outlets were determined. Comparison was made between conventional culture method (BIOLOG) and newly developed PCR assays performed on bacterial isolates (PCR-C), directly on enriched broths (Direct PCR-E) or on DNA extracted from enriched broths (PCR-E) of Campylobacter jejuni, Listeria monocytogenes and Salmonella Typhimurium. Among 150 meat samples (chicken, pork, turkey and beef) tested, Campylobacter, Proteus, Listeria and Salmonella were detected in 27.0, 25.0, 19.0 and 3.3%, respectively, by using BIOLOG. Among 150 samples analyzed, the frequency of detection for C. jejuni, L. monocytogenes and S. Typhimurium were 8.0, 2.7 and 2.0% by both the BIOLOG and PCR-C assays. PCR-C and BIOLOG detected the same number of positive samples, 12 for C. jejuni, 3 for S. Typhimurium, however from the total 4 L. monocytogenes, only 3 were isolated correctly by BIOLOG. Both Direct PCR-E and PCR-E detected 13, 2 and 1 positive samples for C. jejuni, S. Typhimurium and L. monocytogenes, respectively. TaqMan PCR assay on enriched broths produced the most rapid and inhibition-free results compared with Direct PCR-E and PCR-C. The PCR-based methods provided results more rapidly (1-2 days versus 6-7 days) and therefore are recommended for improved detection of these pathogens.

Keywords:

Campylobacter, isolation, Listeria, meat, Salmonella, TaqMan assay

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Nguyen, G., Abo-Samaha, M. I., Reddy, G., Abdulrahman, M., Samuel, T., Adesiyun, A., & Abdela , W. (2015). Improved Detection of Campylobacter jejuni, Listeria monocytogenes and Salmonella Typhimurium from Raw Meat Products Using Conventional and Newly Developed TaqMan Assays. International Journal of Poultry Science, 14(7), 364–375. https://doi.org/10.3923/ijps.2015.364.375
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