Research Article Open Access

Assessment of Pathogenic Potential of Avian Influenza Viruses by MDCK Cell Culture

Authors

CORRESPONDING AUTHOR
B.P. Shankar
High Security Animal Disease Laboratory, IVRI, Bhopal, India
|
R. N. Sreenivas Gowda
Veterinary College, Hebbal, Bangalore-24, Karnataka, India
|
B. Pattnaik
High Security Animal Disease Laboratory, IVRI, Bhopal, India
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B. H. Manjunatha Prabhu
Molecular Biology Genetics Units, JNCASR, Jakkur, Bangalore, India
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R.P. Kamal
High Security Animal Disease Laboratory, IVRI, Bhopal, India
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B.K. Sreenivas
IAH&VB, Hebbal, Bangalore, India
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H.S. Madhusudhan
Veterinary College, Hebbal, Bangalore-24, Karnataka, India
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D. Ranjith
Veterinary College, Hebbal, Bangalore-24, Karnataka, India
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H.K. Pradhan
High Security Animal Disease Laboratory, IVRI, Bhopal, India
Published on: April 15, 2009
DOI 10.3923/ijps.2009.462.464
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Abstract

The influenza viruses are sub-classified in to two pathotypes of Highly Pathogenic Avian Influenza (HPAI) and Low Pathogenic Avian Influenza (LPAI) viruses on the basis of the pathogenicity of AIV in domestic poultry. Samples of H5N1 (7966/06 and 7972/06) and the H9N1 (5844/05) viruses were also grown on MDCK cell. All these produced cytopathic effect within 72 h. Normally, the nonpathogenic AIV does not produce CPE in MDCK cells. However, CPE can be produced if trypsin is incorporated while, culturing the viruses. In the present study, without the addition of trypsin the viruses produced CPE. On FAT both cytoplasmic and nuclear florescence was observed. It is also known that the viruses which produce CPE in absence of trypsin are pathogenic. It proved beyond that the H5N1 and H9N1 viruses isolated in the present study were pathogenic based on cell culture study.

Keywords:

Avian influenza H5N1, CPE, India, MDCK

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Copyright (c) 2009 Asian Network for Scientific Information

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This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

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How to Cite

Shankar, B., Gowda, R. N. S., Pattnaik, B., Prabhu, B. H. M., Kamal, R., Sreenivas, B., Madhusudhan, H., Ranjith, D., & Pradhan, H. (2009). Assessment of Pathogenic Potential of Avian Influenza Viruses by MDCK Cell Culture. International Journal of Poultry Science, 8(5), 462–464. https://doi.org/10.3923/ijps.2009.462.464
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References

1.
Banks, J., E.C. Speidel and D.J. Alexander, 1999. Characterization of non-pathogenic H5N1 (A/ty/Eng/87-92/91) isolate with multiple basic amino acid at the HA cleavage site. Proceedings of the Symposium on Animal Influenza Viruses, Gent, Belgium, May 16-18.
2.
Bosch, F.X., W. Garten, H.D. Klenk and R. Rott, 1981. Proteolytic cleavage of influenza virus haemagglutinins: Primary structure of the connecting peptide between HA1 and HA2 determines proteolytic cleavability and pathogenicity of avian influenza viruses. J. Virol., 113: 725-735.
3.
Gavin, P.J. and R.B. Thomson, Jr., 2003. Review of rapid diagnostic tests for influenza. Clin. Applied Immunol. Rev., 4: 151-172.
4.
Murphy, B.R. and R.G. Webster, 1996. Orthomyxoviruses. In: Field Virology, Fields, B.N., D.M. Knipe and P.M. Howley (Eds.). 3rd Edn., Lippincott, Raven, PA., pp: 1397-1445.
5.
Selleck, P.W., S.L. Lowther, G.M. Russell and P.T. Hooper, 2002. Rapid diagnosis of highly pathogenic avian influenza using pancreatic impression smears. Avian Dis., 47: 1190-1195.

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