Research Article Open Access

Assessment of Pathogenic Potential of Two Indian H5N1 Highly Pathogenic Avian Influenza Virus Isolates by Intravenous Pathogenicity Index Test

Authors

CORRESPONDING AUTHOR
B.P. Shankar
Department of Pathology, Veterinary College, Hebbal, Bangalore - 24, Karnataka, India
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R.N.S. Gowda
Department of Pathology, Veterinary College, Hebbal, Bangalore - 24, Karnataka, India
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B. H. Manjunath Prabhu
Department of Pathology, Veterinary College, Hebbal, Bangalore - 24, Karnataka, India
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B. Pattnaik
Department of Pathology, Veterinary College, Hebbal, Bangalore - 24, Karnataka, India
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S. Nagarajan
High Security Animal Disease Laboratory, IVRI, Bhopal, India
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S.S. Patil
High Security Animal Disease Laboratory, IVRI, Bhopal, India
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H.K. Pradhan
High Security Animal Disease Laboratory, IVRI, Bhopal, India
Published on: February 15, 2009
DOI 10.3923/ijps.2009.283.290
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Abstract

Intravenous Pathogenicity Index (IVPI) test was conducted to assess the pathogenicity of two H5N1 (A/Ck/Ind/7966/06 and A/Ck/Ind/7972/06) AIV isolates. Both the H5N1 virus isolates were isolated from natural outbreaks during 2006, both isolates caused death of all birds with in 48 h after inoculation experimentally, birds showed typical clinical signs, gross and microscopic lesions of Avian influenza. IVPI of A/Ck/Ind/7966/06 and A/Ck/Ind/7972/06 was 2.96 and 2.95 respectively. This test showed that both H5N1 isolates are highly pathogenic. Virus could be re-isolated from all the organs of infected chickens and it was reconfirmed by RT-PCR using WHO and Lee primers.

Keywords:

HPAI, India, ivpi, pathology, rt-pcr

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How to Cite

Shankar, B., Gowda, R., Prabhu, B. H. M., Pattnaik, B., Nagarajan, S., Patil, S., & Pradhan, H. (2009). Assessment of Pathogenic Potential of Two Indian H5N1 Highly Pathogenic Avian Influenza Virus Isolates by Intravenous Pathogenicity Index Test. International Journal of Poultry Science, 8(3), 283–290. https://doi.org/10.3923/ijps.2009.283.290
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