Expression of the Fructose-1,6-Bisphosphatase 1 (FBP1) Gene in the Embryonic Bursa of Fabricius
Authors
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Gregory T. Pharr
College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762-6100, USA
- Hui Wang College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762-6100, USA
- Nikhil Nuthalapati College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA
DOI:
https://doi.org/10.66920/ijps.2026.154.157Keywords:
Bursa of Fabricius, chicken embryo, fructose-1,6-bisphosphatase 1Abstract
Background and Objective: Previous transcriptome analysis of isolated bursal B-cells at Embryonic Day (ED) 16 and ED19 revealed expression of the fructose-1,6-bisphosphatase 1 (FBP1) gene, which encodes a rate-limiting enzyme in the gluconeogenesis (GNG) pathway. To validate this finding, the goal of this project was to confirm FBP1 gene expression before and after the major B-cell differentiation event in the embryonic bursa.
Materials and Methods: Total RNA and frozen sections prepared from pooled bursas of ED15 and ED19 embryos were evaluated using quantitative polymerase chain reaction (qPCR) analysis and in situ hybridization (ISH).
Results: The qPCR results suggest that FBP1 gene expression decreases in the bursa during embryonic development. The ISH methodology localized FBP1 expression to the bursal follicles.
Conclusion: These results confirm the expression of the FBP1 gene during bursal B-cell development. We hypothesize that the expression of a rate-limiting enzyme in the GNG pathway confers metabolic flexibility on bursal B-cells.
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Copyright (c) 2026 Gregory T. Pharr, Hui Wang, Nikhil Nuthalapati
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