Research Article Open Access

Expression of the Fructose-1,6-Bisphosphatase 1 (FBP1) Gene in the Embryonic Bursa of Fabricius

Authors

CORRESPONDING AUTHOR
Gregory T. Pharr
iD 0000-0002-1833-4960
College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762-6100, USA
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Hui Wang
College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762-6100, USA
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Nikhil Nuthalapati
College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA
Published on: September 16, 2026
DOI 10.66920/ijps.2026.154.157
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Abstract

Background and Objective: Previous transcriptome analysis of isolated bursal B-cells at Embryonic Day (ED) 16 and ED19 revealed expression of the fructose-1,6-bisphosphatase 1 (FBP1) gene, which encodes a rate-limiting enzyme in the gluconeogenesis (GNG) pathway. To validate this finding, the goal of this project was to confirm FBP1 gene expression before and after the major B-cell differentiation event in the embryonic bursa.

Materials and Methods: Total RNA and frozen sections prepared from pooled bursas of ED15 and ED19 embryos were evaluated using quantitative polymerase chain reaction (qPCR) analysis and in situ hybridization (ISH).

Results: The qPCR results suggest that FBP1 gene expression decreases in the bursa during embryonic development. The ISH methodology localized FBP1 expression to the bursal follicles.

Conclusion: These results confirm the expression of the FBP1 gene during bursal B-cell development. We hypothesize that the expression of a rate-limiting enzyme in the GNG pathway confers metabolic flexibility on bursal B-cells.

Keywords:

Bursa of Fabricius, chicken embryo, fructose-1,6-bisphosphatase 1

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Copyright (c) 2026 Gregory T. Pharr, Hui Wang, Nikhil Nuthalapati

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This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

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How to Cite

Pharr, G. T., Wang, H., & Nuthalapati, N. (2026). Expression of the Fructose-1,6-Bisphosphatase 1 (FBP1) Gene in the Embryonic Bursa of Fabricius. International Journal of Poultry Science, 25, 154–157. https://doi.org/10.66920/ijps.2026.154.157
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