Effects of Dietary Palm Oil Inclusion Levels on Zootechnical and Biochemical Performance of Pullets


Authors

  • Ahmed MIJIYAWA Department of Animal Science, Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (co-constructed by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding of Shandong Province, College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, 271017, China
  • Elham Ghashghaei ORCiD Department of Animal Science, Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (co-constructed by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding of Shandong Province, College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, 271017, China
  • Okasha Hamada ORCiD Animal Production Department, Faculty of Agriculture, Benha University, Moshtohor 13736, Egypt
  • Huimin Wang ORCiD Department of Animal Science, Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (co-constructed by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding of Shandong Province, College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, 271017, China
  • Uchechukwu Edna Obianwuna ORCiD Institute of Feed Research, Chinese Academy of Agricultural Science, Beijing, China
  • Dossêh Jean Apôtre Afayibo ORCiD Department of Animal Science, Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (co-constructed by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding of Shandong Province, College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, 271017, China
  • Minghui Wang ORCiD Department of Animal Science, Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (co-constructed by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding of Shandong Province, College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, 271017, China
  • Wéré Pitala ORCiD Regional Center of Excellence for Avian Sciences, University of Lome, Lome, Togo
  • Hai Lin ORCiD Department of Animal Science, Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (co-constructed by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Provincial Key Laboratory of Animal Nutrition and Efficient Feeding of Shandong Province, College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong, 271017, China

DOI:

https://doi.org/10.66920/ijps.2026.114.122

Keywords:

Biochemical parameters, palm oil, poultry diet, pullets, zootechnical

Abstract

Objective: This study aimed to improve the production performance of ISA Brown laying hens by incorporating palm oil into their diet.

Materials and Methods: Six hundred ten-week-old birds were randomly allocated to four groups (H0, H1, H2 and H3) and reared for a period of ten weeks. Each group consisted of five replicates of 30 birds each. Groups H0, H1, H2 and H3 were fed diets containing 0, 1, 2 and 3% palm oil, respectively. Body weight, feed intake, average daily gain and feed conversion ratio were measured weekly for all birds. At 21 weeks of age, 10 birds were randomly selected from each group for blood sample collection and subsequent biochemical analysis.

Results: The results indicated no significant differences among the four groups in mean body weight, mean daily gain, feed conversion ratio, or serum gamma-GT concentration (p>0.05). However, feed intake was significantly higher only in group H1 compared with groups H0, H2 and H3 (p<0.05). Groups H2 and H3 exhibited significantly elevated total protein and glucose levels (p<0.0001) compared to groups H0 and H1. In contrast, ALT concentration was significantly higher in group H3 than in groups H0, H1 and H2 (p<0.0001). Triglyceride levels were also significantly higher (p<0.0001) in the treated groups compared with group H0. Moreover, AST and total cholesterol concentrations were markedly elevated in group H3.

Conclusion: Based on these results, the incorporation of 2% palm oil into pullet diets is recommended to improve production performance.

References

Bouvarel, I., Y. Nys, M. Panheleux and P. Lescoat, 2010. How diet influences the quality of eggs. INRAE Prod. Anim., 23: 167-182.

National Research Council, 1994. Nutrient Requirements of Poultry. 9th ed., National Academies Press, Washington, DC, USA, ISBN: 978‑0‑309‑04892‑7. Pages:176.

Zitari, S, 2005. Etude des valeurs nutritives de certaines ressources alimentaires locales utilisées dans l'alimentation des animaux. Master's Thesis. Université de Sousse.

Panda, A.K., B. Prakash, S.V.R. Rao, M.V.L.N. Raju and G.S. Sunder, 2013. Utilisation of high quality protein maize in poultry. World's Poult. Sci. J., 69: 877-888.

DOI: https://doi.org/10.1017/S0043933913000871

Ani, A.O., C. Baes, P. Chemineau, M. Gauly, R. Jiménez-Flores and A. Rosati et al, 2021. Opinion paper: COVID-19 and the livestock sector. Animal, Vol. 15. 10.1016/j.animal.2020.100102

DOI: https://doi.org/10.1016/j.animal.2020.100102

Murray, A.G., S.C. Ives, R.J. Smith and M. Moriarty, 2021. A preliminary assessment of indirect impacts on aquaculture species health and welfare in Scotland during COVID-19 lockdown. Vet. Anim. Sci., Vol. 11. 10.1016/j.vas.2021.100167

DOI: https://doi.org/10.1016/j.vas.2021.100167

Uyanga, V.A., O.M. Onagbesan, C.F.I. Onwuka, B. Emmanuel and H. Lin, 2021. Coronavirus disease 2019 (COVID-19) and poultry production: Emerging issues in African countries. World's Poult. Sci. J., 77: 153-174.

DOI: https://doi.org/10.1080/00439339.2021.1874848

Mijiyawa, A., E. Ghashghaei, J. Zhu, T. Dou, L. Sun and H. Lin et al, 2026. Effect of incorporation level of palm kernel meal and rearing age on apparent metabolizable energy for broiler. Poult. Sci., Vol. 105. 10.1016/j.psj.2025.106107

DOI: https://doi.org/10.1016/j.psj.2025.106107

Halle, I, 1996. Effect of dietary fat on performance and fatty acid composition of egg yolk in laying hens. Eur. Poult. Sci., 60: 65-72.

DOI: https://doi.org/10.1016/S0003-9098(25)01306-2

Harms, R.H., G.B. Russell and D.R. Sloan, 2000. Performance of four strains of commercial layers with major changes in dietary energy. J. Appl. Poult. Res., 9: 535-541.

DOI: https://doi.org/10.1093/japr/9.4.535

Grobas, S., J. Mendez, C.D. Blas and G.G. Mateos, 1999. Laying hen productivity as affected by energy, supplemental fat, and linoleic acid concentration of the diet. Poult. Sci., 78: 1542-1551.

DOI: https://doi.org/10.1093/ps/78.11.1542

Gab, W.B, 1992. Contribution à l'étude de l'influence de la qualité et de la quantité des lipides alimentaires sur les performances de croissance et l'état d'engraissement des poulets de chair. Master's Thesis. Université Cheikh Anta Diop.

Antoniou, T., R.R. Marquardt and R. Misir, 1980. The utilization of rye by growing chicks as influenced by calcium, vitamin D3, and fat type and level. Poult. Sci., 59: 758-769.

DOI: https://doi.org/10.3382/ps.0590758

Machin, D.H., S. Panigrahi, J. Bainton and T.R. Morris, 1990. Performance of broiler chicks fed on low and high oil fish silages in relation to changes taking place in lipid and protein components. Anim. Feed Sci. Technol., 28: 199-223.

DOI: https://doi.org/10.1016/0377-8401(90)90153-Y

Frttsche, K.L., N.A. Cassity and S.-C. Huang, 1991. Effect of dietary fats on the fatty acid compositions of serum and immune tissues in chickens. Poult. Sci., 70: 1213-1222.

DOI: https://doi.org/10.3382/ps.0701213

Ravindran, V., P. Tancharoenrat, F. Zaefarian and G. Ravindran, 2016. Fats in poultry nutrition: Digestive physiology and factors influencing their utilisation. Anim. Feed Sci. Technol., 213: 1-21.

DOI: https://doi.org/10.1016/j.anifeedsci.2016.01.012

Pan, P.R., B.C. Dilworth, E.J. Day and T.C. Chen, 1979. Effect of season of the year, sex, and dietary fats on broiler performance, abdominal fat, and preen gland secretion. Poult. Sci., 58: 1564-1574.

DOI: https://doi.org/10.3382/ps.0581564

Mba, O.I., M.-J. Dumont and M. Ngadi, 2015. Palm oil: Processing, characterization and utilization in the food industry – A review. Food BioSci., 10: 26-41.

DOI: https://doi.org/10.1016/j.fbio.2015.01.003

Morin, O. and X. Pagès-Xatart-Parès, 2012. Vegetable oils & fats: functional resources and nutritional interest. Ol. Corps Gras, Lipides, 19: 63-75.

DOI: https://doi.org/10.1051/ocl.2012.0446

Sambanthamurthi, R., K. Sundram and Y.-A. Tan, 2000. Chemistry and biochemistry of palm oil. Prog. Lipid Res., 39: 507-558.

DOI: https://doi.org/10.1016/S0163-7827(00)00015-1

Ghafoorunissa,, 1995. Nutrition & health implications of palm oil in Indian diets. Indian J. Med. Res., 102: 233-240.

Sundram, K., R. Sambanthamurthi and Y.-A. Tan, 2003. Palm fruit chemistry and nutrition. Asia Pac. J. Clin. Nutr., 12: 355-362.

Selvaduray, K.R., A.K. Radhakrishnan, M.K. Kutty and K. Nesaretnam, 2011. Palm tocotrienols decrease levels of pro-angiogenic markers in human umbilical vein endothelial cells (HUVEC) and murine mammary cancer cells. Genes Nutr., 7: 53-61.

DOI: https://doi.org/10.1007/s12263-011-0223-0

Yam, M., S.R.A. Hafid, H. Cheng and K. Nesaretnam, 2009. Tocotrienols suppress proinflammatory markers and cyclooxygenase‐2 expression in RAW264.7 macrophages. Lipids, 44: 787-797.

DOI: https://doi.org/10.1007/s11745-009-3326-2

Serbinova, E.A. and L. Packer, 1994. Antioxidant and biological activities of palm oil vitamin E. Food Nutr. Bull., Vol. 15. 10.1177/156482659401500213

DOI: https://doi.org/10.1177/156482659401500213

Muharis, S.P., A.G.M. Top, D. Murugan and M.R. Mustafa, 2010. Palm oil tocotrienol fractions restore endothelium dependent relaxation in aortic rings of streptozotocin-induced diabetic and spontaneously hypertensive rats. Nutr. Res., 30: 209-216.

DOI: https://doi.org/10.1016/j.nutres.2010.03.005

Sen, C.K., C. Rink and S. Khanna, 2010. Palm oil–derived natural vitamin E α-tocotrienol in brain health and disease. J. Am. Coll. Nutr., 29: 314-323.

DOI: https://doi.org/10.1080/07315724.2010.10719846

Koreleski, J., M. Kuchta, R. Rys and A. Sieradzka, 1993. Comparison of the influence of rapeseed oil and fish fat in laying hen nutrition on the level of polyunsaturated fatty acids in egg yolk. Agric. Food Sci.

Leclercq, B. and M. Larbier, 1991. Nutrition et alimentation des volailles. 1st ed., Éditions Quae, Versailles, France, ISBN: 9782759238620, 9782759238637, 9782759238644. Pages: 366.

Jensen, L.S., I. Bartov, M.J. Beirne, J.R. Veltmann and D.L. Fletcher, 1980. Reproduction of the oily bird syndrome in broilers. Poult. Sci., 59: 2256-2266.

DOI: https://doi.org/10.3382/ps.0592256

Fuller, H.L. and M. Rendon, 1979. Energetic efficiency of corn oil and poultry fat at different levels in broiler diets. Poult. Sci., 58: 1234-1238.

DOI: https://doi.org/10.3382/ps.0581234

Blum, J.-C. and B. Leclercq, 1979. Influence du niveau énergétique et de la granulation du régime sur les performances de croissance et l’engraissement du pintadeau. Comparaison avec le poulet. Ann. zootechnie, 28: 261-269.

DOI: https://doi.org/10.1051/animres:19790304

Dale, N.M. and H.L. Fuller, 1979. Effect of low temperature, diet density, and pelleting on the preference of broilers for high fat rations. Poult. Sci., 58: 1337-1339.

DOI: https://doi.org/10.3382/ps.0581337

Akinwande, A.I, 1981. Influence of dietary fat on growth and liver lipid content, glucose 6-phosphate and 6-phosphogluconate dehydrogenases, and aldolase activities in the chick. Poult. Sci., 60: 1259-1263.

DOI: https://doi.org/10.3382/ps.0601259

Brown, H.B. and M.G. McCartney, 1982. Effects of dietary energy and protein and feeding time on broiler performance. Poult. Sci., 61: 304-310.

DOI: https://doi.org/10.3382/ps.0610304

Olomu, J.M. and S.A. Offiong, 1980. The effects of different protein and energy levels and time of change from starter to finisher ration on the performance of broiler chickens in the tropics. Poult. Sci., 59: 828-835.

DOI: https://doi.org/10.3382/ps.0590828

Fuller, H.L. and N.M. Dale, 1982. Effect of ratio of basal diet fat to test fat on the true metabolizable energy of the test fat. Poult. Sci., 61: 914-918.

DOI: https://doi.org/10.3382/ps.0610914

Coon, C.N., W.A. Becker and J.V. Spencer, 1981. The effect of feeding high energy diets containing supplemental fat on broiler weight gain, feed efficiency, and carcass composition. Poult. Sci., 60: 1264-1271.

DOI: https://doi.org/10.3382/ps.0601264

Blundell, J, 1991. Pharmacological approaches to appetite suppression. Trends Pharmacological Sci., 12: 147-157.

DOI: https://doi.org/10.1016/0165-6147(91)90532-W

Woods, S.C., R.J. Seeley, D. Porte and M.W. Schwartz, 1998. Signals that regulate food intake and energy homeostasis. Science., 280: 1378-1383.

DOI: https://doi.org/10.1126/science.280.5368.1378

Katongole, J.B.D. and B.E. March, 1980. Fat utilization in relation to intestinal fatty acid binding protein and bile salts in chicks of different ages and different genetic sources. Poult. Sci., 59: 819-827.

DOI: https://doi.org/10.3382/ps.0590819

Cheng, T.K., A. Peguri, M.L. Hamre and C.N. Coon, 1991. Effect of rearing regimens on pullet growth and subsequent laying performance. Poult. Sci., 70: 907-916.

DOI: https://doi.org/10.3382/ps.0700907

Crespo, N. and E. Esteve-Garcia, 2001. Dietary fatty acid profile modifies abdominal fat deposition in broiler chickens. Poult. Sci., 80: 71-78.

DOI: https://doi.org/10.1093/ps/80.1.71

Okandza, Y., P. Mopoundza, S.D. Ngatse, M. Halbouche and P. Akouango, 2017. Influence de la substitution graduelle de tourteau de soja par la féverole sur la croissance et la conformation de la carcasse chez les poulets de chair. J. Appl. Biosci., 110: 10714-10720.

DOI: https://doi.org/10.4314/jab.v110i1.2

Bigot, K., M. Taouis and S. Tesseraud, 2003. Refeeding and insulin regulate S6K1 activity in chicken skeletal muscles. J. Nutr., 133: 369-373.

DOI: https://doi.org/10.1093/jn/133.2.369

Portugaliza, H.P. and T.J. Fernandez Jr, 2012. Growth performance of Cobb broilers given varying concentrations of malunggay (Moringa oleifera Lam.) aqueous leaf extract. Online J. Anim. Feed Res., 2: 465-469.

Griffin, C.C.W.H.D, 1984. Development of divergent lines of lean and fat broilers using plasma very low density lipoprotein concentration as selection criterion: The first three generations. Br. Poult. Sci., 25: 573-582.

DOI: https://doi.org/10.1080/00071668408454899

Tete–Benissan, A., M.-L.A. Quashie, K. Lawson-Evi, K. Kokou and M. Gbeassor, 2012. Récupération nutritionnelle chez les sujets malnutris VIH positifs et VIH négatifs après utilisation de feuilles de Moringa oleifera Lam. J. Anim. Plant Sci. s, 15: 2184-2199.

Eckersall, P.D, 2008. Proteins, proteomics, and the dysproteinemias. In: Clinical Biochemistry of Domestic Animals, Kaneko, J.J., J.W. Harvey and M.L. Bruss, (Eds.). Elsevier, San Diego, California, USA, pp: 117-155.

DOI: https://doi.org/10.1016/B978-0-12-370491-7.00005-2

Harrison, G.J. and L.R. Harrison, 1986. Clinical avian medicine and surgery, including aviculture. 1st ed., Saunders, Philadelphia, USA, ISBN: 0721612415, 978-0721612416. Pages: 717.

Botham, K.M. and P.A. Mayes, 2023. Oxidation of fatty acids: Ketogenesis. In: Harper's Illustrated Biochemistry, Kennelly, P.J., K.M. Botham, O.P. McGuinness, V.W. Rodwell and P.A. Weil, (Eds.). McGraw Hill Education, New York, USA, pp: 217-225.

Nkosi, C.Z., A.R. Opoku and S.E. Terblanche, 2005. Effect of pumpkin seed (Cucurbita pepo) protein isolate on the activity levels of certain plasma enzymes in CCL4-induced liver injury in low-protein fed rats. Phytother. Res., 19: 341-345.

DOI: https://doi.org/10.1002/ptr.1685

Jaensch, S., 2000. Diagnosis of avian hepatic disease. Semin. Avian Exot. Pet Med., 9: 126-135.

DOI: https://doi.org/10.1053/ax.2000.7140

Vernon, R.G., M.C. Barber and M.T. Travers, 1999. Développements récents dans les études de la lipogenèse chez l’Homme et chez les animaux. INRA Prod. Anim., 12: 319-327.

DOI: https://doi.org/10.20870/productions-animales.1999.12.4.3893

Ding, Y., X. Bu, N. Zhang, L. Li and X. Zou, 2016. Effects of metabolizable energy and crude protein levels on laying performance, egg quality and serum biochemical indices of Fengda-1 layers. Anim. Nutr., 2: 93-98.

DOI: https://doi.org/10.1016/j.aninu.2016.03.006

Mensink, R.P. and M.B. Katan, 1992. Effect of dietary fatty acids on serum lipids and lipoproteins. A meta-analysis of 27 trials.. Arteriosclerosis Thrombosis: A J. Vascular Biol., 12: 911-919.

Mensink, R.P. and M.B. Katan, 1992. Effect of dietary fatty acids on serum lipids and lipoproteins. A meta-analysis of 27 trials. Arterioscler. Thromb.: J. Vasc. Biol., 12: 911-919.

DOI: https://doi.org/10.1161/01.ATV.12.8.911

Onibi, G. and A. Bobadoye, 2011. Haematological indices, serum cholesterol and meat quality of broiler chickens fed diets with palm oil sludge substituting maize. Agric. Biol. J. North Am., 2: 552-558.

DOI: https://doi.org/10.5251/abjna.2011.2.3.552.558

Downloads

Published

2026-07-28

Issue

Section

Research Article

How to Cite

MIJIYAWA, A., Ghashghaei, E. ., Hamada, O. ., Wang, H. ., Obianwuna, U. E. .,  Afayibo, D., Wang, M. ., Pitala, W. ., & Lin, H. . (2026). Effects of Dietary Palm Oil Inclusion Levels on Zootechnical and Biochemical Performance of Pullets. International Journal of Poultry Science, 25, 114–122. https://doi.org/10.66920/ijps.2026.114.122