Avian E2A Expression Level Variation Associated with Phenotypic Variation of Two Chinese Indigenous Duck Breeds, (Anas platyrhynchos)


Authors

  • Dafaalla M. Mekki Deparment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China Department of Animal Science, Faculty of Natural Resources and Environmental Studies, Sudan
  • Bian Liang-yong Deparment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China
  • Wang Jin-yu Deparment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China
  • Yang Yan Deparment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China
  • Yu Yabo Deparment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China
  • Hassan H. Musa Deparment of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiangsu, China

DOI:

https://doi.org/10.3923/ijps.2006.1023.1028

Keywords:

Avian E2A, duck breeds, gene expression, semi-quantitative RT-PCR

Abstract

Semi-quantitative polymerase chain reaction (RT-PCR) was applied to quantify and compare variations in avian E2A expression level between two Chinese indigenous duck breeds for different tissues. Six primer combinations in addition to beta-actin as an internal control were used. The expression level of E2A was significantly (p< 0.05) higher in kidney tissues than lung tissues for both Gao you and Kun shan indigenous duck breeds. The variability of gene expression between two breeds showed 34.35% and 60.70% higher expression in Gao you breed than Kun shan breed for kidney and lung tissues, respectively. Differences were found to be significant (p< 0.05) only for expression level in lung tissues. No significant effect of sex upon E2A expression was detected. The association between E2A relative expression level and body weight was found to be positive and moderate for the genetically lean breed (Gao you), while it was negative and has low values for genetically heavy breed (Kun shan).

Downloads

Published

2006-10-15

Issue

Vol. 5 No. 11 (2006)

Section

Research Article

License

Copyright (c) 2006 Asian Network for Scientific Information

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

How to Cite

Mekki, D. M., Liang-yong, B., Jin-yu, W., Yan, Y., Yabo, Y., & Musa, H. H. (2006). Avian E2A Expression Level Variation Associated with Phenotypic Variation of Two Chinese Indigenous Duck Breeds, (Anas platyrhynchos). International Journal of Poultry Science, 5(11), 1023–1028. https://doi.org/10.3923/ijps.2006.1023.1028