Evaluation of Genetic Diversity and Genetic Distance Between Twelve Chinese Indigenous Chicken Breeds Based on Microsatellite Markers


Authors

  • Yu Ya-Bo Institute of Poultry Science: Chinese Academy of Agriculture Science, Yangzhou 225003, Jiancsu, China, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiancsu, China
  • Wang Jin-Yu College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiancsu, China
  • D.M. Mekki College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiancsu, China Faculty of Natural Resources and Environmental Studies, University of Kordofan, Elobeid 51 1 1, Sudan
  • Tang Qing-Ping Institute of Poultry Science: Chinese Academy of Agriculture Science, Yangzhou 225003, Jiancsu, China
  • Li Hui-Fang Institute of Poultry Science: Chinese Academy of Agriculture Science, Yangzhou 225003, Jiancsu, China
  • Gu Rong Institute of Poultry Science: Chinese Academy of Agriculture Science, Yangzhou 225003, Jiancsu, China
  • Ge Qing-Lian Institute of Poultry Science: Chinese Academy of Agriculture Science, Yangzhou 225003, Jiancsu, China
  • Zhu Wen-Qi College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiancsu, China
  • Chen Kuan-Wei Faculty of Natural Resources and Environmental Studies, University of Kordofan, Elobeid 51 1 1, Sudan

DOI:

https://doi.org/10.3923/ijps.2006.550.556

Keywords:

Chicken breeds, genetic distance, genetic diversity, microsatellite

Abstract

A total of 720 individuals of 12 indigenous chicken populations, geographically localized in Southern China were genotyped for 30 microsatellite markers in polymerase chain reaction (PCR) to evaluate the genetic variation and genetic distance between populations. All microsatellites were found to be polymorphic. Heterozygosity was calculated to determine the genetic variation. Of the 30 microsatellite loci, number of alleles per locus (Na) and effective number of alleles per locus (Ne) ranged from 4 to 11 and 2.157 to 8.019, respectively. The average expected heterozygosity (HE) was 0.669, while the average observed heterozygosity (HO) was 0.764. The polymorphism information content (PIC) has values between 0.560 and 0.641. Using Nei`s standard distance, genetic distance (DA) calculated ranged between 0.088 (Guanxi Sanhuang vs. Nandan Yao) and 0.495 (Huiyang Beard vs. Zhangzhou Game). The topology of phylogenetic trees constructed showed general patterns of relationship and genetic differentiation among the indigenous populations studied, however, both trees from Neighbor-Joining method and Unweighted Pair Group method showed a similar topology. The results provided evidence of the applicability of microsatellite to determining the genetic relatedness among different Chinese indigenous chicken populations and evaluating of genetic variations.

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Published

2006-05-15

Issue

Vol. 5 No. 6 (2006)

Section

Research Article

License

Copyright (c) 2006 Asian Network for Scientific Information

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

How to Cite

Ya-Bo, Y., Jin-Yu, W., Mekki, D., Qing-Ping, T., Hui-Fang, L., Rong, G., Qing-Lian, G., Wen-Qi, Z., & Kuan-Wei, C. (2006). Evaluation of Genetic Diversity and Genetic Distance Between Twelve Chinese Indigenous Chicken Breeds Based on Microsatellite Markers. International Journal of Poultry Science, 5(6), 550–556. https://doi.org/10.3923/ijps.2006.550.556

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