Differential Expression Levels Assessment of Avian E2A Gene in Two Chinese Indigenous Duck Breeds (Anas platyrhynchos) by Semi-Quantitative RT-PCR


Authors

  • Dafaalla M. Mekki Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, Jiancsu, China 2 Department of Animal Science, Faculty of Natural Resources and Environmental Studies, Sudan
  • Bian Liang-yong Department of Animal Science, Faculty of Natural Resources and Environmental Studies, University of Kordofan, North Kordofan State, Sudan
  • Wang Jin-yu Department of Animal Science, Faculty of Natural Resources and Environmental Studies, University of Kordofan, North Kordofan State, Sudan
  • Yang Yan Department of Animal Science, Faculty of Natural Resources and Environmental Studies, University of Kordofan, North Kordofan State, Sudan
  • Li Lin-chuan Department of Animal Science, Faculty of Natural Resources and Environmental Studies, University of Kordofan, North Kordofan State, Sudan
  • Yu Yabo Department of Animal Science, Faculty of Natural Resources and Environmental Studies, University of Kordofan, North Kordofan State, Sudan
  • Hassan H. Musa Department of Animal Science, Faculty of Natural Resources and Environmental Studies, University of Kordofan, North Kordofan State, Sudan

DOI:

https://doi.org/10.3923/ijps.2006.895.899

Keywords:

Duck breeds, E2A, gene expression, semi-quantitative RT-PCR

Abstract

Semi-quantitative polymerase chain reaction (RT-PCR) was applied to quantify and compare variations in avian E2A expression level between two Chinese indigenous duck breeds for different tissues. Six primer combinations in addition to β-actin as an internal control were used. The expression level of E2A was significantly higher (p< 0.05) in kidney tissues than lung tissues for both Gao you and Kun shan indigenous duck breeds. The variability of gene expression between two breeds showed 34.35% and 60.70% higher expression in Gao you breed than Kun shan breed for kidney and lung tissues, respectively. Differences were found to be significant (p< 0.05) only for expression level in lung tissues. No significant effect of sex upon E2A expression was detected.

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Published

2006-08-15

Issue

Vol. 5 No. 9 (2006)

Section

Research Article

License

Copyright (c) 2006 Asian Network for Scientific Information

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

How to Cite

Mekki, D. M., Liang-yong, B., Jin-yu, W., Yan, Y., Lin-chuan, L., Yabo, Y., & Musa, H. H. (2006). Differential Expression Levels Assessment of Avian E2A Gene in Two Chinese Indigenous Duck Breeds (Anas platyrhynchos) by Semi-Quantitative RT-PCR. International Journal of Poultry Science, 5(9), 895–899. https://doi.org/10.3923/ijps.2006.895.899