The Recognition of a vlhA Protein from the F-Strain of Mycoplasma gallisepticum with Monoclonal Antibody 6F10


Authors

  • G.T. Pharr Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA
  • S.L. Branton 1JSDA, ARS, South Central Poultry Research Unit, Mississippi state: MS 39762, USA
  • A.M. Cooksey Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA
  • L.A. Hanson Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA
  • S.C. Burgess Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA
  • C.C. Bottoms Department of Math and Natural Sciences, Collin County Community College, Frisco, TX 75035, USA
  • F.M. McCarthy Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA
  • S.D. Collier 1JSDA, ARS, South Central Poultry Research Unit, Mississippi state: MS 39762, USA

DOI:

https://doi.org/10.3923/ijps.2006.789.795

Keywords:

F-strain, mass spectrometry, monoclonal antibody 6F10, Mycoplasma gallisepticum, vlhA

Abstract

The goal of this project is to identify the genes encoding M. gallisepticum F-strain surface proteins recognized by specific antibody reagents to characterize the individual role of each gene product in host colonization. Here we report the characterization of a 70-kDa surface protein recognized by monoclonal antibody 6F10. The surface protein was gel isolated and digested with trypsin for evaluation with mass spectrometry. The results showed that 19 out of the 21 tryptic peptides matched with proteins of the vlhA lipoprotein family. We suggest that monoclonal antibody 6F10 is specific for the predominantly expressed vlhA protein (formerly pMGA) of the F-strain of M. gallisepticum.

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Published

2006-07-15

Issue

Vol. 5 No. 8 (2006)

Section

Research Article

License

Copyright (c) 2006 Asian Network for Scientific Information

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

How to Cite

Pharr, G., Branton, S., Cooksey, A., Hanson, L., Burgess, S., Bottoms, C., McCarthy, F., & Collier, S. (2006). The Recognition of a vlhA Protein from the F-Strain of Mycoplasma gallisepticum with Monoclonal Antibody 6F10. International Journal of Poultry Science, 5(8), 789–795. https://doi.org/10.3923/ijps.2006.789.795

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