Genotypic Characterization of Avian Escherichia coli by Random Amplification of Polymorphic DNA
DOI:
https://doi.org/10.3923/ijps.2005.378.381Keywords:
E. coli, pathogenic strains, poultry industry, RAPD-PCRAbstract
Fifteen serotypes of E. coli isolated from poultry were subjected to RAPD-PCR using eight oligonucleotide random primers. Amplification with each of the primers resulted in generation of different DNA fingerprinting profile with varied number of bands. Dendrogram based generation of clustering of E. coli serotypes showed two major clusters. The polymorphic amplicons served as markers for E. coli species where as the unique amplicons served as markers for E. coli serotypes. RAPD based fingerprinting provided a rapid means of discriminating E. coli serotypes and considered a valuable tool for molecular typing.
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Copyright (c) 2005 Asian Network for Scientific Information
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This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
