Production of an Extra Cellular Feather Degrading Enzyme by Bacillus licheniformis Isolated from Poultry Farm Soil in Namakkal District (Tamilnadu)


Authors

  • P. Tamilmani Department of Biochemistry, P.G.P College Art and Science, Namakkal, Tamilnadu, India
  • A. Umamaheswari Department of Biotechnology, Prince Shri Venkateshwara Arts and Science College, Chennai, Tamilnadu, India
  • A. Vinayagam Department of Biochemistry, P.G.P College Art and Science, Namakkal, Tamilnadu, India
  • B. Prakash Department of Biochemistry, P.G.P College Art and Science, Namakkal, Tamilnadu, India

DOI:

https://doi.org/10.3923/ijps.2008.184.188

Keywords:

Bacillus licheniformis, feather waste, fermentation and keratinase producation, keratin

Abstract

Chicken feather is recognized as a solid waste generated from poultry farms and is abundant in Namakkal district, Tamilnadu, India. Feather is commonly treated by high temperature and pressure; it is used as animal food stuffs. However, feather degradation by biological methods has been increasingly interested because of environmental awareness. In this study, unidentified bacterial strains isolated from soil samples. They had show ability of feather degradation by making a clear zone around their colonies in FMA medium. The zoned isolates were identified by morphological and biochemical tests. The Bacillus licheniformis were also examined for keratinase production by shake flask fermentation in a basal medium containing 1% feather. The fermentation mediums were optimized. Fermentation process was carried out at 37°C for 7 days at 150 rpm. Crude keratinase were extracted and purified by salt precipitation, dialysis and column chromatography and measured the activity of keratinase.

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Published

2008-01-15

Issue

Vol. 7 No. 2 (2008)

Section

Research Article

License

Copyright (c) 2008 Asian Network for Scientific Information

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

How to Cite

Tamilmani, P., Umamaheswari, A., Vinayagam, A., & Prakash, B. (2008). Production of an Extra Cellular Feather Degrading Enzyme by Bacillus licheniformis Isolated from Poultry Farm Soil in Namakkal District (Tamilnadu) . International Journal of Poultry Science, 7(2), 184–188. https://doi.org/10.3923/ijps.2008.184.188