The Effect of Substrate Composition Fermented Using Pleurotus ostreatus on the Nutrient Content of Palm Oil Sludge
DOI:
https://doi.org/10.3923/ijps.2019.323.327Keywords:
Cellulase, crude fiber, crude protein, fermentation, laccase, palm oil sludge, Pleurotus ostreatusAbstract
Background and Objective: Palm oil sludge has been an alternative feed ingredient for poultry but its utilization is limited due to its high content of crude fiber (lignin and cellulose) and its low crude protein content. Fermentation with Pleurotus ostreatus (lignocellulolytic fungus producing the enzyme ligninase) was used to degrade the crude fiber to increase the nutritional content of fermented palm oil sludge. This study aimed to determine the best substrate composition of palm oil sludge fermented by Pleurotus ostratus. Materials and Methods: The completely randomized design (CRD) was used with 4 treatments and 5 replications. The treatments consisted of palm oil sludge (POS) and rice bran (RB): A (100% POS+0% RB), B (90% POS+10% RB), C (80% POS+20% RB) and D (70% POS+30% RB). The parameters measured were crude protein (%), crude fiber (%), cellulase enzymatic activity (U mL–1) and laccase enzymatic activity (U mL–1). Results: Fermentation with Pleurotus ostreatus showed a highly significant effect (p<0.01) on crude protein content, a significant effect (p<0.05) on crude fiber content and no significant effects (p>0.05) on the enzymatic activities of cellulase and laccase. Conclusion: It is concluded that treatment B increased the crude protein, cellulase activity and laccase activity in palm oil sludge but decreased the crude fiber content.
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