Expression and Subcellular Location of a Leucine Aminopeptidase of Mycoplasma Gallinarum


Authors

  • X. Wan Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA
  • S.L. Branton Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA
  • M.B. Hughlett Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA
  • L.A. Hanson Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA
  • G.T. Pharr Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA

DOI:

https://doi.org/10.3923/ijps.2004.70.74

Keywords:

Commensal, leucine aminopeptidase, Mycoplasma gallinarum, subcellular location

Abstract

Mycoplasma Gallinarum is a commensal with a host range that includes most poultry. This property of M. gallinarum may reflect unique mechanisms for colonization and persistence in various hosts. In previous studies a leucine aminopeptidase (LAP) gene candidate was cloned from a M. gallinarum recombinant genomic library and characterized. Here we evaluate the LAP gene from M. gallinarum at the protein level. A recombinant fusion protein was purified and employed as an antigen to immunize chickens to obtain polyclonal anti-LAP serum. The antiserum was utilized to identify the subcellular location by immuoblotting with Triton X-114 partitions of M. gallinarum proteins. Our results of these experiments show that the LAP gene product is located in the cytoplasmic fraction of the M. gallinarum cell.

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Published

2003-12-15

Issue

Vol. 3 No. 1 (2004)

Section

Research Article

License

Copyright (c) 2004 Asian Network for Scientific Information

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

How to Cite

Wan, X., Branton, S., Hughlett, M., Hanson, L., & Pharr, G. (2003). Expression and Subcellular Location of a Leucine Aminopeptidase of Mycoplasma Gallinarum. International Journal of Poultry Science, 3(1), 70–74. https://doi.org/10.3923/ijps.2004.70.74

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