A Simple Protocol for Amplification of Genes from Inactivated Oil Adjuvant Fowl Cholera Vaccine
Abstract
Pasteurellosis is one of the most contagious disease of avians. Control of pasteurellosis is at present carried out by vaccination with whole-cell bacterins, which confer serotype specific protection. Outermembrane proteins (OMP) of Gram negative bacteria are considered the most important immunogens and have been proved to confer immunity. A simple protocol for amplification of outermembrane protein genes from inactivated bacterial vaccines has been standardized. An attempt has been made to compare the OMP genes of field isolates and vaccine strain.
Keywords:
OMPH gene, Pasteurella multocida, restriction enzyme analysis, vaccine strainArticle Metrics
License
Copyright (c) 2006 Asian Network for Scientific Information
This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
Article Citation Metrics
How to Cite
