Detection of InvA Gene in Isolated Salmonella            from Broilers by PCR Method

T. Zahraei Salehi; M. Mahzounieh; A. Saeedzadeh

doi:10.3923/ijps.2005.557.559

Research Article Open Access

Detection of InvA Gene in Isolated Salmonella from Broilers by PCR Method

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Published on: July 15, 2005

DOI 10.3923/ijps.2005.557.559

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Abstract

The presence of Salmonella was detected in 192 samples of poultry carcasses from poultry farms in Shiraz province (Iran). A total of 30 Salmonella isolates were found in chicken samples (15.6%), by conventional culturing and confirmed by PCR and serology methods. Strains of serogroup D1 were the most prevalent strains, followed by serogroups C1, B and C2. All strains were subjected to Salmonella-specific gene (invA) and were confirmed as Salmonella positive by the predicted product a 284-bp DNA fragment.

Keywords:

Broiler, invA gene, Iran, polymerase chain reaction (PCR), Salmonella

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This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

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Salehi, T. Z., Mahzounieh, M., & Saeedzadeh, A. (2005). Detection of InvA Gene in Isolated Salmonella from Broilers by PCR Method. International Journal of Poultry Science, 4(8), 557–559. https://doi.org/10.3923/ijps.2005.557.559