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The Effects of Mycotoxins, Toxin Binder and Deactivator on the Hematology, Serum Chemistry and Immune Response of Broiler Chickens Vaccinated Against Newcastle Disease

Authors

CORRESPONDING AUTHOR
Eze, Chekwube Paul
iD 0000-0001-7181-1925
Department of Veterinary Pathology and Microbiology, University of Nigeria, Nsukka, Nigeria
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Vincent Olu. Shoyinka
Department of Veterinary Pathology and Microbiology, University of Nigeria, Nsukka, Nigeria
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Wilfred Sunday Ezema
Department of Veterinary Pathology and Microbiology, University of Nigeria, Nsukka, Nigeria
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Kennedy Foinkfu Chah
Department of Veterinary Pathology and Microbiology, University of Nigeria, Nsukka, Nigeria
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Anthony Christian Mgbeahuruike
Department of Veterinary Pathology and Microbiology, University of Nigeria, Nsukka, Nigeria
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Emmanuel Aniebonam Eze
Department of Microbiology, University of Nigeria, Nsukka, Nigeria
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Innocent Okonkwo Ogbonna
Department of Microbiology, Joseph Sarwuan Tarka University, Makurdi, Benue State, Nigeria
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Adaeze Suzzy Udeh
Department of Biological Science, Godfrey Okoye University, Enugu, Nigeria
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Chosen Ekene Obih
Department of Biological Science, Godfrey Okoye University, Enugu, Nigeria
Published on: September 15, 2025
DOI 10.3923/ijps.2025.53.62
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Abstract

Objectives: This study was designed to investigate the effects of mycotoxins and toxin binder and deactivator on the hematology, serum chemistry and immune response of broiler chickens vaccinated against Newcastle disease. Materials and Methods: The finisher feed was moistened with water, and stored in bags for 21 days. It was divided into three parts: A, B, and C. Part A and part B was treated with toxin binder (Toxorid®) and deactivator (Zerotox®) respectively. Part C was not treated with toxin binder or deactivator. The contaminated feeds were stored at 25-30°C for 2 weeks before use. Fresh uncontaminated and untreated feed, ‘D’ served as the positive control and all evaluated for fungal growth and mycotoxins. A total of 224 broilers were used in this study. Broilers of 5-weeks old were divided into eight groups and fed experimental finisher feeds (A, B, C, D). Two weeks Post Exposure (PE), groups AK, BK, CK and DK were revaccinated with ND vaccine (Komarov®). The hematocrit, erythrocyte counts, hemoglobin concentration, mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration of groups A, B, C and D were ascertained. The total and differential leukogram of groups A, B, C and D were determined. The Aspartate Aminotransferase (AST) activity, uric acid, total serum protein, serum albumin and serum globulin concentrations of groups A, B, C and D were determined. The HI titers of all the groups were obtained. The data obtained were subjected to Analysis of Variance (ANOVA). Results: The phenotypic characterization, DNA extraction and Polymerase Chain Reaction (PCR) amplification of the feed samples revealed that the isolates recovered from groups A, B, C and D were mycotoxigenic fungi. The mean total aflatoxins detected via competitive ELISA were 1.25 ppb for A, 0.97 ppb for B, 2.14 ppb for C and 0.73 ppb for D. The hemoglobin and mean corpuscular hemoglobin concentration of group D, at week 9 of age, was significantly (p<0.05) higher than C, but not (p>0.05) significant with A and B. There was significant increase in (p<0.05) leukocyte counts in groups C, A and B when compared with the control group (D) between weeks 2-4 PE. In the differential leukogram, (p<0.05) marked heterophils, eosinophils, monocytes and lymphocytes were seen in groups C, A and B when compared with D between weeks 2-4 PE. Uric acid concentrations of group C at week 2 PE (2.35±0.38) was significantly lower (p<0.05) than those of D (3.58±0.60), B (7.01±2.24) and A (4.17±0.47). The total serum protein and the serum albumin concentrations of group C was significantly (p<0.05) lower than, A and B, at week 4 PE. The serum globulin and serum albumin globulin ratio concentrations presented the same pattern as seen above. The HI titres of the revaccinated groups, (AK, BK, CK and DK) increased (p<0.05) significantly when compared with groups A, B, C and D between weeks 3-5 PE. Conclusion: The emanating hematological, HI titers, and biochemical lesions observed among the exposed groups indicated the extent of systemic damage by mycotoxins and ameliorating effects of binders in salvaging contaminated feed. This research outcome is a useful guide to policy formulators, farmers, nutritionists and researchers.

Keywords:

Antibody response, binders, blood chemistry, chickens, contaminated feed, hematology, mycotoxins

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Eze, C. P., Shoyinka, V. O., Ezema, W. S., Chah, K. F., Mgbeahuruike, A. C., Eze, E. A., Ogbonna, I. O., Suzzy Udeh, A., & Obih, C. E. (2025). The Effects of Mycotoxins, Toxin Binder and Deactivator on the Hematology, Serum Chemistry and Immune Response of Broiler Chickens Vaccinated Against Newcastle Disease. International Journal of Poultry Science, 24, 53-62. https://doi.org/10.3923/ijps.2025.53.62
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References

1.
FAO, 2018. Africa Sustainable Livestock (ASL) 2050 Livestock and livelihoods spotlight - Nigeria. Cattle and poultry sectors. 1st ed., Food and Agriculture Organization, Rome, Italy, Pages:12.
2.
Ajala, A., S. Ogunjimi, O. Famuwagun and A. Adebimpe, 2021. Poultry production in Nigeria: Exploiting its potentials for rural youth empowerment and entrepreneurship. Niger. J. Anim. Prod., 48: 114-123.
3.
Alexander, D.J., 2001. Newcastle disease. Br. Poult. Sci., 42: 5-22.
4.
Getabalew, M., T. Alemneh, D. Akeberegn, D. Getahun and D. Zewdi, 2019. Epidemiology, diagnosis & prevention of Newcastle disease in poultry. Am. J. BioMed. Sci. Res., 3: 50-59.
5.
AHA, 2014. Disease Strategy: Newcastle Disease. 3rd ed., Agriculture Ministers’ Forum, Canberra, ACT, Canberra, Pages:74.
6.
Vidanović, D., M. Šekler, R. Ašanin, N. Milić, J. Nišavić and V. Savić et al, 2011. Characterization of velogenic Newcastle disease viruses isolated from dead wild birds in Serbia during 2007. J. Wildl. Dis., 47: 433-441.
7.
Alexander, D.J., J.G. Bell and R.G. Alders, 2004. A Technology Review: Newcastle Disease - With Special Emphasis on Its Effects on Village Chickens. Food and Agriculture Organization (FAO), Rome, ISBN: 92-5-105080-5, Pages:63.
8.
Terregino, C., G. Cattoli, B. Grossele, E. Bertoli, E. Tisato and I. Capua, 2003. Characterization of Newcastle disease virus isolates obtained from EurAsian collared doves (Streptopelia decaocto) in Italy. Avian Pathol., 32: 63-68.
9.
Absalón, A.E., D.V. Cortés-Espinosa, E. Lucio, P.J. Miller and C.L. Afonso, 2019. Epidemiology, control, and prevention of Newcastle disease in endemic regions: Latin America. Trop. Anim. Health Prod., 51: 1033-1048.
10.
Eze, C.P., V.S.O. Shoyinka, J.O.A. Okoye, W.S. Ezema, I.O. Ogbonna and O.K. Ikejiofor et al, 2014. Comparison of the serum proteins and immune responses of velogenic Newcastle disease virus infected chickens and ducks. Open J. Vet. Med., 4: 122-128.
11.
Eze, C.P., J.O.A. Okoye, I.O. Ogbonna, W.S. Ezema, D.C. Eze and K.I. Idika et al, 2014. Comparative evaluation of the effects of velogenic Newcastle disease virus infection on the hematology of ducks and chickens. Open J. Vet. Med., 4: 113-121.
12.
EZE, C.P., J.O.A. Okoye, I.O. Ogbonna, W.S. Ezema, D.C. EZE and E.A. Salihu et al, 2013. Comparative study of the pathology and pathogenesis of a local velogenic Newcastle disease virus infection in ducks and chickens. Int. J. Poult. Sci., 13: 52-61.
13.
RUMA, 2006. Responsible use of vaccines and vaccination in poultry production.
15.
Okwor, E.C., D.C. Eze, G.O.N. Echeonwu, J.O. Ibu, P.C. Eze and J.O.A. Okoye, 2016. Comparative studies on the effects of la sota and komarov vaccine antibodies on organ distribution, persistence and shedding of kudu 113 virus in chickens. J. Anim. Plant Sci., 26: 1226-1235.
16.
FAO, 2013. The State of Food and Agriculture 2013: Food System for Better Nutrition. 1st ed., Food and Agriculture Organization, Rome, Italy, ISBN: 978-92-5-107671-2, Pages:114.
17.
Pereira, C.S., S.C. Cunha and J.O. Fernandes, 2019. Prevalent mycotoxins in animal feed: Occurrence and analytical methods. Toxins, Vol. 11. 10.3390/toxins11050290
18.
Nazhand, A., A. Durazzo, M. Lucarini, E.B. Souto and A. Santini, 2020. Characteristics, occurrence, detection and detoxification of aflatoxins in foods and feeds. Foods, Vol. 9. 10.3390/foods9050644
19.
Awuchi, C.G., E.N. Ondari, C.U. Ogbonna, A.K. Upadhyay, K. Baran and R.P.F. Guiné et al, 2021. Mycotoxins affecting animals, foods, humans, and plants: Types, occurrence, toxicities, action mechanisms, prevention, and detoxification strategies—A revisit. Foods, Vol. 10. 10.3390/foods10061279
20.
Shnawa, A.R.B.H, 2021. The pernicious activity of aflatoxin on biochemical and reproductive parameters of commercial male birds: A short review. Acad. Lett.,. 10.20935/al3480
21.
CAST, 2003. Mycotoxins: Risks in Plant, Animal, and Human Systems. Council for Agricultural Science and Technology, Ames, Iowa, USA, ISBN: 1-887383-22-0, Pages:199.
22.
Refai, M.K., A. Elbatrawi, G. Osman and A. Hassan, 2016. Monograph on Avian Mycoses & Mycotoxicoses: A guide for postgraduate students. 1st ed., Faculty of Veterinary Medicine, Cairo University, Cairo, Pages:675.
23.
Reddy, K.R.N., B. Salleh, B. Saad, H.K. Abbas, C.A. Abel and W.T. Shier, 2010. An overview of mycotoxin contamination in foods and its implications for human health. Toxin Revs., 29: 3-26.
24.
Ezekiel, C., M. Sulyok, D. Babalola, B. Warth, V. Ezekiel and R. Krska, 2013. Incidence and consumer awareness of toxigenic Aspergillus section Flavi and aflatoxin B1 in peanut cake from Nigeria. Food Control, 30: 596-601.
25.
Mgbeahuruike, A.C., T.E. Ejioffor, O.C. Christian, V.C. Shoyinka, M. Karlsson and E. Nordkvist, 2018. Detoxification of aflatoxin-contaminated poultry feeds by 3 adsorbents, bentonite, activated charcoal, and fuller’s earth. J. Appl. Poult. Res., 27: 461-471.
26.
Anthony, C.M., I.N. Emmanuela and O.S.I. Onwumere, 2020. A survey of the aflatoxin level and molecular identification of fungal contaminants in poultry feed mills from different geopolitical zones of Nigeria. Afr. J. BioTechnol., 19: 500-507.
27.
Kemboi, D.C., P.E. Ochieng, G. Antonissen, S. Croubels, M.-L. Scippo and J.K. Gathumbi et al, 2020. Multi-mycotoxin occurrence in dairy cattle and poultry feeds and feed ingredients from Machakos town, Kenya. Toxins, Vol. 12. 10.3390/toxins12120762
28.
Raja, H.A., A.N. Miller, C.J. Pearce and N.H. Oberlies, 2017. Fungal identification using molecular tools: A primer for the natural products research community. J. Nat. Prod., 80: 756-770.
29.
Shoyinka, S.V.O., K.F. Chah, C.P. Eze, W.S. Ezema, I.R. Onoja and P.U. Umeakuana, 2014. Bioassay procedure for the diagnosis of aflatoxicosis in a pig farm in Nsukka, South East Nigeria. Open J. Vet. Med., 4: 129-133.
30.
Ezekiel, C.N., O.A. Oyedele, B. Kraak, K.I. Ayeni, M. Sulyok and R. Krska et al, 2020. Fungal diversity and mycotoxins in low moisture content ready-to-eat foods in Nigeria. Front MicroBiol., Vol. 11. 10.3389/fmicb.2020.00615
31.
Ochieng, P.E., M.-L. Scippo, D.C. Kemboi, S. Croubels, S. Okoth and G. Antonissen et al, 2021. Mycotoxins in poultry feed and feed ingredients from Sub-Saharan Africa and their impact on the production of broiler and layer chickens: A review. Toxins, Vol. 13. 10.3390/toxins13090633
32.
Muneer, M., M. Bilal and A. Ditta, 2021. A comparative study of some hematological parameters of broiler and indigenous breeds of poultry. Svu-Int. J. Agric. Sci., 3: 189-198.
33.
Afolabi, K.D., A.O. Akinsoyinu, A.-R.O. Abdullah, R. Olajide and S.B. Akinleye, 2011. Haematological parameters of the Nigerian local grower chickens fed varying dietary levels of palm kernel cake. Poljoprivreda, 17: 74-78.
34.
Murugesan, G.R., D.R. Ledoux, K. Naehrer, F. Berthiller, T.J. Applegate and G. Schatzmayr et al, 2015. Prevalence and effects of mycotoxins on poultry health and performance, and recent development in mycotoxin counteracting strategies. Poult. Sci., 94: 1298-1315.
35.
Saminathan, M., J. Selamat, A.A. Pirouz, N. Abdullah and I. Zulkifli, 2018. Effects of nano-composite adsorbents on the growth performance, serum biochemistry, and organ weights of broilers fed with aflatoxin-contaminated feed. Toxins, Vol. 10. 10.3390/toxins10090345
36.
Nazarizadeh, H. and J. Pourreza, 2019. Evaluation of three mycotoxin binders to prevent the adverse effects of aflatoxin B1 in growing broilers. J. Appl. Anim. Res., 47: 135-139.
37.
Gil-Serna, J., M. García-Díaz, C. Vázquez, M.T. González-Jaén and B. Patiño, 2019. Significance of Aspergillus niger aggregate species as contaminants of food products in Spain regarding their occurrence and their ability to produce mycotoxins. Food MicroBiol., 82: 240-248.
38.
Rout, N., B.K. Nanda and S. Gangopadhyaya, 1989. Experimental pheohyphomycosis and mycotoxicosis by Curvularia lunata in albino rats. Indian J. Pathol. MicroBiol., 32: 1-6.
39.
Pham, J., B. Kulla and M. Johnson, 2022. Invasive fungal infection caused by Curvularia species in a patient with intranasal drug use: A case report. Med. Mycol. Case Rep., 37: 1-3.
40.
FAO, 2018. Africa Sustainable Livestock (ASL) 2050 Livestock and livelihoods spotlight - Nigeria: Cattle and poultry sectors. Food and Agriculture Organization, Rome, Italy, Pages:12.
41.
Pierron, A., I. Alassane-Kpembi and I.P. Oswald, 2016. Impact of mycotoxin on immune response and consequences for pig health. Anim. Nutr., 2: 63-68.
42.
DSM-Firmenich, 2024. World Mycotoxin Survey: The Global Threat. January – December 2023. dsm-firmenich (Animal Nutrition & Health division).
44.
Al-Nedawi, A.M, 2018. Reference hematology for commercial Ross 308 broilers. Online J. Vet. Res., 22: 566-570.
45.
Campbell, T.W. and C.K. Ellis, 2015. Exotic animal hematology and cytology. 4th ed., Wiley-Blackwell, Ames, Iowa, USA, 9781118611272,9781118993705, Pages:432.
46.
Vajpayee, N., S.S. Graham and S. Bem, 2007. Basic Examination of Blood and Bone Marrow. In: Henry's Clinical Diagnosis and Management by Laboratory Methods, McPherson, R. and M. Pincus, (Eds.). Elsevier Saunders, Philadelphia, PA, USA, pp: 510-539.
47.
Elghetany, M.T. and K. Banki, 2017. Erythrocytic Disorders. In: Henry's Clinical Diagnosis and Management by Laboratory Methods, McPherson, R. and M. Pincus, (Eds.). Elsevier Saunders, Philadelphia, PA, USA, pp: 559-605.
48.
Maner, B.S., R.B. Killeen and L. Moosavi, 2024. Mean Corpuscular Volume. In: StatPearls [Internet]. StatPearls Publishing, Florida, United States.
49.
Harrison, L., C. Brown, C. Afonso, J. Zhang and L. Susta, 2011. Early occurrence of apoptosis in lymphoid tissues from chickens infected with strains of newcastle disease virus of varying virulence. J Comp Pathol, 145: 327-335.
50.
Dash, S.P., S. Gupta and P.P. Sarangi, 2024. Monocytes and macrophages: Origin, homing, differentiation, and functionality during inflammation. Heliyon, Vol. 10. 10.1016/j.heliyon.2024.e29686
51.
Mehrzad, J., S. Shojaei, F. Keivan, D. Forouzanpour, H. Sepahvand and P. Hooshmand et al, 2024. Avian innate and adaptive immune components: A comprehensive review. J. Poult. Sci. Avian Dis., 2: 73-96.
52.
Thomson, R.G, 1984. General Veterinary Pathology. W.B. Saunders, Philadelphia, Pennsylvania, USA, IS0721688519, Pages:463.
53.
Lichtman, M.A, 2012. Monocytosis and monocytopenia. In: Williams Hematology, Lichtman, M.A, (Ed.). McGraw-Hill Education, New York, NY, USA.
54.
Zachary, J.F, 2017. Pathologic Basis of Veterinary Disease. 6th ed., Elsevier, St. Louis, Missouri, USA. ISBN: 978-0-323-35775-3, Pages:1394.
55.
Lam, K.M, 1996. Growth of Newcastle disease virus in chicken macrophages. J. Comp. Pathol., 115: 253-263.
56.
Thrall, M.A., G. Weiser, R.W. Allison and T.W. Campbell, 2012. Veterinary hematology and clinical chemistry. 2nd ed., Wiley-Blackwell, John Wiley & Sons, Inc.,, Ames, IA, USA, ISBN: 978-0-8138-1027-0, Pages:776.
57.
Fadly, A.M., J.R. Glisson, L.R. McDougald, L.K. Nolan and D.E. Swayne, 2008. Diseases of Poultry. 12th ed., Wiley-Blackwell, Ames, Iowa, USA, ISBN: 978-0813807188, Pages:1324.
58.
Villegas, P. and H.G. Purchase, 1989. Titration of Biological Suspension. In: A Laboratory Manual for the Isolation and Identification of Avian Pathogens,. American Association of Avian Pathologists, Iowa, USA, pp: 186-190.
59.
Nester, W.E., G.D. Anderson, C.R. Evans (Jr.), N.N. Pearsall, M.T. Nester and D. Hurley, 2004. Microbiology: A Human Perspective. 8th ed., McGraw-Hill Education, New York, NY, USA, ISBN: 10: 0073522597, Pages:896.
60.
Talaro, K.P, 2005. Foundations in Microbiology: Basic Principles. 5th ed., Mcgraw-Hill, Boston, Massachusetts, USA, Pages:850.

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