The Effect of Different Extenders on Some Fertility Properties of Roosters Semen
Abstract
Background and Objective: Poultry sperm gradually loses its quality within an hour of sample collection. This study aimed to determine the storage effects of different extenders (at 4°C) on rooster sperm fertility properties. Materials and Methods: Eighteen seminal fluid samples of a Lohman Brown strain were collected by dorsal-abdominal massage. These were divided into three groups and added to three extenders: a simple medium for assisted reproductive technology (SMART), Tris and milk. Since non-diluted fresh sperm loses its quality within an hour of semen collection, no control group was used. Percentages of mass activity, motility, normality and viability were tested. These were done at 1, 4 and 8 h after semen collection. Completely Random Design (C.R.D) was used in this experiment. Results: At the first timepoint, the semen test score of the SMART medium extender was higher than that of the other extenders. In contrast, the milk extender shows a highly significant improvement in sperm parameters post preservation at both the second and the third time tests. The SMART extender was protecting sperm vigor more than Tris at most of the tested timepoints. Conclusion: Extenders delay the loss of rooster sperm fertilization ability. Full milk extender was better than other extenders in the protection of sperm fertility.
Keywords:
Mass activity, milk extender, rooster sperm, semen motility, semen viabilityArticle Metrics
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