Research Article Open Access

Association Study and Expression Analysis of Stearoyl Co-A Desaturase as a Candidate Gene for Fatty Acid Composition in Indonesian Crossbred Chickens

Authors

Asep Gunawan
Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University, 16680 Bogor, Indonesia
|
Eva Siti Nurajizah
Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University, 16680 Bogor, Indonesia
|
Kasita Listyarini
Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University, 16680 Bogor, Indonesia
|
Ahmad Furqon
Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University, 16680 Bogor, Indonesia
|
Woki Bilyaro
Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University, 16680 Bogor, Indonesia
|
Cece Sumantri
Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University, 16680 Bogor, Indonesia
|
Jakaria
Department of Animal Production and Technology, Faculty of Animal Science, Bogor Agricultural University, 16680 Bogor, Indonesia
|
Syeda Hasina Akter
School of Veterinary Science, University of Queensland, Gatton Campus, Qld 4343, Australia
|
CORRESPONDING AUTHOR
Muhammad Jasim Uddin
School of Veterinary Science, University of Queensland, Gatton Campus, Qld 4343, Australia
Published on: June 15, 2018
DOI 10.3923/ijps.2018.348.355
PDF

Abstract

Background and Objective: The stearoyl-CoA desaturase (SCD) gene encodes an enzyme involved in fatty acid (FA) biosynthesis. The aim of the present study was to investigate the association and expression of SCD as a candidate gene for FA composition in Indonesian crossbred chickens. Materials and Methods: Sixty-two Indonesian crossbred, unsexed chickens (F2 Kampung×broilers) were used. FA composition was measured at 12 weeks of age from thigh meat of crossbred chicken. Results: A single nucleotide polymorphism in coding region c.17492542 C>G of SCD was associated with FA composition, including both unsaturated [linoleic (C18:2n6c) and eicosadienoic (C20:2) acids] and saturated [lauric acid (C12:0)] forms. SCD mRNA expression analysis in liver revealed 6 chickens with extremely high and low FA compositions, of them, high FA birds (n = 3) had higher unsaturated and lower saturated FAs, while the low FA group (n = 3) had lower unsaturated and higher saturated FA levels. SCD expression was higher (p<0.05) in tissues collected from high FA chickens than low FA chickens. Conclusion:These results will improve the understanding of SCD function in FA composition and will shed light on SCD as a potential candidate in the selection of chickens with higher levels of unsaturated and lower levels of saturated FA.

Keywords:

Fatty acid composition, Indonesian crossbred chickens, liver, mRNA, polymorphism

Article Metrics

120 Abstract Views
96 File Views
0 Downloads

License

Copyright (c) 2018 The Author(s)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

Article Citation Metrics

Find Association Study and Expression Analysis of Stearoyl Co-A Desaturase as a Candidate Gene for Fatty Acid Composition in Indonesian Crossbred Chickens on PlumX

How to Cite

Gunawan, A., Nurajizah, E. S., Listyarini, K., Furqon, A., Bilyaro, W., Sumantri, C., Jakaria, Akter, S. H., & Uddin , M. J. (2018). Association Study and Expression Analysis of Stearoyl Co-A Desaturase as a Candidate Gene for Fatty Acid Composition in Indonesian Crossbred Chickens. International Journal of Poultry Science, 17(7), 348–355. https://doi.org/10.3923/ijps.2018.348.355
Crossref
Scopus
Google Scholar
Europe PMC

References

1.
Yu, K., G. Shu, F. Yuan, X. Zhu and P. Gao et al., 2013. Fatty acid and transcriptome profiling of longissimus dorsi muscles between pig breeds differing in meat quality. Int. J. Biol. Sci., 9: 108-118.
2.
Cameron, N.D., M. Enser, G.R. Nute, F.M. Whittington and J.C. Penman et al., 2000. Genotype with nutrition interaction on fatty acid composition of intramuscular fat and the relationship with flavour of pig meat. Meat Sci., 55: 187-195.
3.
Furqon, A., A. Gunawan, N. Ulupi, T. Suryati and C. Sumantri, 2017. Expression and association of SCD gene polymorphisms and fatty acid compositions in chicken cross. Med. Pet., 40: 151-157.
4.
Prasad, M.R. and V.C. Joshi, 1979. Purification and properties of hen liver microsomal terminal enzyme involved in stearoyl coenzyme A desaturation and its quantitation in neonatal chicks. J. Biol. Chem., 254: 6362-6368.
5.
Maharani, D., H.B. Park, J.B. Lee, C.K. Yoo and H.T. Lim et al., 2013. Association of the gene encoding stearoyl-CoA desaturase (SCD) with fatty acid composition in an intercross population between Landrace and Korean native pigs. Mol. Biol. Rep., 40: 73-80.
6.
Matsuhashi, T., S. Maruyama, Y. Uemoto, N. Kobayashi and H. Mannen et al., 2011. Effects of bovine fatty acid synthase, stearoyl-coenzyme A desaturase, sterol regulatory element-binding protein 1 and growth hormone gene polymorphisms on fatty acid composition and carcass traits in Japanese black cattle. J. Anim. Sci., 89: 12-22.
7.
Ohsaki, H., A. Tanaka, S. Hoashi, S. Sasazaki and K. Oyama et al., 2009. Effect of SCD and SREBP genotypes on fatty acid composition in adipose tissue of Japanese black cattle herds. Anim. Sci. J., 80: 225-232.
8.
Taniguchi, M., T. Utsugi, K. Oyama, H. Mannen and M. Kobayashi et al., 2004. Genotype of stearoyl-CoA desaturase is associated with fatty acid composition in Japanese Black cattle. Mamm. Genome, 14: 142-148.
9.
Uemoto, Y., T. Abe, N. Tameoka, H. Hasebe and K. Inoue et al., 2010. Whole‐genome association study for fatty acid composition of oleic acid in Japanese black cattle. Anim. Genet., 42: 141-148.
10.
Folch, J., M. Lees and G.H.S. Stanley, 1957. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem., 226: 497-509.
11.
Jeong, J., X. Shi, X. Zhang, S. Kim and C. Shen, 2011. An empirical Bayes model using a competition score for metabolite identification in gas chromatography mass spectrometry. BMC Bioinform., Vol. 12.
12.
Gunawan, A., K. Kaewmala, M.J. Uddin, M.U. Cinar and D. Tesfaye et al., 2011. Association study and expression analysis of porcine ESR1 as a candidate gene for boar fertility and sperm quality. Anim. Reprod. Sci., 128: 11-21.
13.
Rozen, S. and H. Skaletsky, 2000. Primer3 on the WWW for general users and for biologist programmers. Methods Mol. Biol., 132: 365-386.
14.
Silver, N., S. Best, J. Jiang and S.L. Thein, 2006. Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR. BMC Mol. Biol., Vol. 7.
15.
Cinar, M.U., A. Kayan, M.J. Uddin, E. Jonas and D. Tesfaye et al., 2012. Association and expression quantitative trait loci (eQTL) analysis of porcine AMBP, GC and PPP1R3B genes with meat quality traits. Mol. Biol. Rep., 39: 4809-4821.
16.
Kayan, A., M.U. Cinar, M.J. Uddin, C. Phatsara and K. Wimmers et al., 2011. Polymorphism and expression of the porcine Tenascin C gene associated with meat and carcass quality. Meat Sci., 89: 76-83.
17.
Nestel, P., P. Clifton and M. Noakes, 1994. Effects of increasing dietary palmitoleic acid compared with palmitic and oleic acids on plasma lipids of hypercholesterolemic men. J. Lipid Res., 35: 656-662.
18.
Grundy, S.M., 1994. Influence of stearic acid on cholesterol metabolism relative to other long-chain fatty acids. Am. J. Clin. Nutr., 60: 986S-990S.
19.
Warensjo, E., J. Sundstrom, B. Vessby, T. Cederholm and U. Riserus, 2008. Markers of dietary fat quality and fatty acid desaturation as predictors of total and cardiovascular mortality: A population-based prospective study. Am. J. Clin. Nutr., 88: 203-209.
20.
Lu, P., D. Li, J. Yin, L. Zhang and Z. Wang, 2008. Flavour differences of cooked longissimus muscle from Chinese indigenous pig breeds and hybrid pig breed (Duroc x Landrace x Large white). Food Chem., 107: 1529-1537.
21.
Tapiero, H., G.N. Ba, P. Couvreur and K.D. Tew, 2002. Polyunsaturated fatty acids (PUFA) and eicosanoids in human health and pathologies. Biomed. Pharmacotherap., 56: 215-222.
22.
Gebauer, S.K., T.L. Psota, W.S. Harris and P.M. Kris-Etherton, 2006. n-3 Fatty acid dietary recommendations and food sources to achieve essentiality and cardiovascular benefits. Am. J. Clin. Nutr., 83: S1526-S1535.
23.
Mauvoisin, D. and C. Mounier, 2011. Hormonal and nutritional regulation of SCD1 gene expression. Biochimie, 93: 78-86.
24.
Merino, D.M., D.W.L. Ma and D.M. Mutch, 2010. Genetic variation in lipid desaturases and its impact on the development of human disease. Lipids Health Dis., Vol. 9.
25.
Patton, K.K., P.T. Ellinor, S.R. Heckbert, R.H. Christenson, C. DeFilippi, J.S. Gottdiener and R.A. Kronmal, 2009. N-terminal pro-B-type natriuretic peptide is a major predictor of the development of atrial fibrillation: The cardiovascular health study. Circulation, 120: 1768-1774.
26.
Nitambi, J.M. and M. Miyazaki, 2004. Regulation of stearoyl-CoA desaturases and role in metabolism. Prog. Lipid Res., 43: 91-104.
27.
Miyazaki, M. and J.M. Ntambi, 2003. Role of stearoyl-coenzyme a desaturase in lipid metabolism. Prostaglandins Leukot Essent Fatty Acids, 68: 113-121.
28.
Hulbert, A.J., 2010. Metabolism and longevity: Is there a role for membrane fatty acids? Integr. Comp. Biol., 50: 808-817.
29.
Uemoto, Y., H. Nakano, T. Kikuchi, S. Sato and M. Ishida et al., 2011. Fine mapping of porcine SSC14 QTL and SCD gene effects on fatty acid composition and melting point of fat in a Duroc purebred population. Anim. Genet., 43: 225-228.
30.
Ren, J., C. Knorr, L. Huang and B. Brenig, 2004. Isolation and molecular characterization of the porcine stearoyl-CoA desaturase gene. Gene, 340: 19-30.
31.
Quintanilla, R., R.N. Pena, D. Gallardo, A. Canovas and O. Ramirez et al., 2011. Porcine intramuscular fat content and composition are regulated by quantitative trait loci with muscle-specific effects. J. Anim. Sci., 89: 2963-2971.
32.
Sanchez, M.P., N. Iannuccelli, B. Basso, J.P. Bidanel and Y. Billon et al., 2007. Identification of QTL with effects on intramuscular fat content and fatty acid composition in a Duroc × Large white cross. BMC Genet., Vol. 8.
33.
Dobrzyn, P., A. Dobrzyn, M. Miyazaki, P. Cohen and E. Asilmaz et al., 2004. Stearoyl-CoA desaturase 1 deficiency increases fatty acid oxidation by activating AMP-activated protein kinase in liver. Proc. Natl. Acad. Sci. USA., 101: 6409-6414.
34.
Mashek, D.G. and R.A. Coleman, 2006. Cellular fatty acid uptake: The contribution of metabolism. Curr. Opin. Lipidol., 17: 274-278.

Most read articles by the same author(s)