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Egg Injection of Ascorbic Acid Stimulates Leucocytosis and Cell Proliferation in the Bursa of Fabricius
Abstract
Broiler chicks with high immune defense potential at hatching are of great value to the poultry industry. Heat stress can have immunosuppressive or immune stimulatory effect and Ascorbic Acid (AA) acts as anti-stressor and has important physiological functions in bird immune system development. This study aimed to assess the effect of intra egg injection of AA before incubation on development of the bursa of Fabricius and leukocyte parameters in chicks from eggs incubated at thermoneutral and hot temperatures. Fertile eggs from broiler breeder chickens were used in a 5 × 2 factorial design with 5 treatments (non-injected and thus non-perforated and injected with 0, 2, 4 and 6% AA in 100 μL of water) and 2 incubation temperatures (thermoneutral: 37.5°C and hot: 39.0°C). The total leukocyte count; percentages of thrombocytes, basophils, eosinophils, monocytes and heterophils; weight; and bursal index remained unaffected by incubation temperature and AA dose. Bursal follicle area decreased in chicks from non-injected eggs when incubated at hot temperatures. With intra egg injection of 4 and 6% AA, bursal follicle size increased in eggs incubated at thermoneutral temperature and decreased at hot temperature compared to no AA injection. Cell proliferation in follicles increased by injecting 2% AA, whereas the percentage of lymphocytes increased by injecting 4 and 6% AA. The data show that AA acts as a dose- and temperature-dependent immunomodulator and that 4 and 6% AA doses stimulate egg bursal development and lymphocytosis. These findings suggest that intra egg injection of AA produces chicks with higher potential for immune defense against challenges in the field.
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Chicks, cloacal sac, leucocytes, mitosis, vitamin CArticle Metrics
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Copyright (c) 2013 Asian Network for Scientific Information
This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
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Sgavioli, S., Borges, L. L., Almeida, V. R. de, Thimotheo, M., Oliveira, J. A. de, & Boleli , I. C. (2013). Egg Injection of Ascorbic Acid Stimulates Leucocytosis and Cell Proliferation in the Bursa of Fabricius. International Journal of Poultry Science, 12(8), 464–472. https://doi.org/10.3923/ijps.2013.464.472
References
1.
Alloui, M.N., S. Sellaoui and S. Djaaba, 2005. Morphometrical and anatomo-pathological survey of the bursa of fabricius in broiler chickens. ISAH., 2: 52-55.
2.
Altan, O., A. Altan, M. Cabuk and H. Bayraktar, 2000. Effects of heat stress on some blood parameters in broilers. Turk. J. Vet. Anim. Sci., 24: 145-148.
3.
Brake, J.T., 1989. Role of ascorbic acid in poultry production: ascorbic acid, stress and immunity. Zootech. Int. Jan., 1: 37-40.
4.
Briggs Jr., G.M., T.D. Luckey, C.A. Elvehjem and E.B. Hart, 1944. Effect of. ascorbic acid on chick growth when added to purified rations. Exp. Biol. Med., 55: 130-134.
5.
Campo, J.L. and S.G. Davila, 2002. Changes in heterophils to lymphocyte ratios of heat-stressed chickens in response to dietary supplementation of several related stress agents. Eur. Poult. Sci., 66: 80-84.
6.
Celis, J.E. and A. Celis, 1985. Cell cycle-dependent variations in the distribution of the nuclear protein cyclin proliferating cell nuclear antigen in cultured cells: Subdivision of S phase. Proc. Natl. Acad. Sci., 82: 3262-3266.
7.
Cheville, N.F., 1978. Environmental factors affecting the immune response of birds. Avian Dis., 23: 308-314.
8.
Coelho, M.B. and J.L. McNaughton, 1995. Effect of composite vitamin supplementation on broilers. J. Appl. Poult. Res., 4: 219-229.
9.
Coles, E.H., 1986. Veterinary Clinical Pathology. 4th Edn., W.B. Saunders Co., Philadelphia, London, ISBN: 978-0721618289, Pages: 486.
10.
Compton, M.M., P.S. Gibbs and L.R. Swicegood, 1990. Glucocorticoid-mediated activation of DNA degradation in avian lymphocytes. Gen. Comp. Endocrinol., 80: 68-79.
11.
Cunningham, J.G., 1999. Tratado de Fisiologia Veterinaria. Elsevier Health Sciences, Rio de Janeiro, Guanabara, Pages: 454.
12.
Dieterlen-Lievre, F., 1994. Hemopoiesis during avian ontogeny. Poult. Sci. Rev., 5: 273-305.
13.
Donker, R.A. and G. Beuving, 1989. Effect of corticosterone infusion on plasma corticosterone concentration, antibody production, circulating leukocytes and growth in chicken lines selected for humoral immune responsiveness. Br. Poult. Sci., 30: 361-369.
14.
El-Boushy, A.R., 1988. Vitamin E affects viability, immune response of poultry. Feedstuffs, 60: 20-24, 26.
15.
Furlan, R.L. and M. Macari, 2002. Termorregulacao. In: Fisiologia Aviaria: Aplicada a Frangos de Corte, Macari, M., R.L. Furlan and E. Gonzales (Eds.). 2nd Edn., Funep, Jaboticabal, pp: 209-230.
16.
Ghonim, A.I.A., A.L. Awad, M.H.A. Fattouh and A.M. El-Shhat, 2009. Comparative study of ascorbic acid treatment methods on hatchability traits and growth performance of ducklings. Egypt. Poult. Sci. J., 29: 1085-1099.
17.
Glick, B., 2000. Immunophysiology. In: Sturkie's Avian Physiology, Whittow, G.C. (Ed.). 5th Edn., Academic Press, San Diego, USA., ISBN-13: 9780127476056, Pages: 685.
18.
Glick, B., 1995. Embryogenesis of the bursa of Fabricius: Stem cell, microenvironment and receptor-paracrine pathways. Poult. Sci., 74: 419-426.
19.
Gross, W.B. and H.S. Siegel, 1983. Evaluation of the heterophil/lymphocyte ratio as a measure of stress in chickens. Avian Dis., 27: 972-979.
20.
Kidd, M.T., 2004. Nutritional modulation of immune function in broilers. Poult. Sci., 83: 650-657.
21.
Kincade, P.W. and M.D. Cooper, 1977. Immunoglobulin A: Site and sequence of expression in developing chicks. Science, 179: 398-400.
22.
Macari, M. and B.C. Luquetti, 2002. Fisiologia Cardiovascular. In: Fisiologia Aviaria Aplicada a Frangos de Corte, Macari, M., R.L. Furlan and E. Gonzales (Eds.). 2nd Edn., FUNEP/UNESP, Jaboticabal, Brazil, ISBN-13:9788587632487, pp: 17-36.
23.
Mahmoud, K.Z., F.W. Edens, E.J. Eisen and G.B. Havenstein, 2004. Ascorbic acid decreases heat shock protein 70 and plasma corticosterone response in broilers (Gallus gallus domesticus) subjected to cyclic heat stress. Comp. Biochem. Physiol. Part B: Biochem. Mol. Biol., 137: 35-42.
24.
Miller, L. and M.A. Qureshi, 1991. Introduction of heat shock proteins and phagocytic function of chicken macrophage following in vitro heat exposure. Vet. Immunol. Immunopathol., 37: 34-42.
25.
Miyachi, K., M.J. Fritzler and E.M. Tan, 1978. Autoantibody to a nuclear antigen in proliferating cells. J. Immunol., 121: 2228-2234.
26.
Miltenburg, G., 1999. Avicultura. AVIC Profes., 17: 33-35.
27.
Morgulis, M.S., 2002. Imunologia Aplicada. In: Fisiologia Aviaria Aplicada a Frangos de Corte. Macari, M., R.L. Furlan and E. Gonzales (Eds.). FUNEP/UNESP, Jaboticabal, Brazil, pp: 231-245.
28.
Motoyama, N., F. Wang, K.A. Roth, H. Sawa and K. Nakayama et al., 1995. Massive cell death of immature hematopoietic cells and neurons in Bcl-x-deficient mice. Science, 267: 1506-1509.
29.
Natt, P.M. and C.A. Herrik, 1952. A new blood diluent for counting the erythrocytes and leucocytes of chicken. Poult. Sci., 31: 735-738.
30.
Paramithiotis, E. and M.J.H. Ratcliffe, 1994. Survivors of bursal B cell production and emigration. Poult. Sci., 73: 991-997.
31.
Pardue, S.L. and J.P. Thaxton, 1984. Evidence for amelioration of steroid-mediated immunosuppression by ascorbic acid. Poult. Sci., 63: 1262-1268.
32.
Pardue, S.L., J.P. Thaxton and J. Brake, 1985. Role of ascorbic acid in chicks exposed to high environmental temperature. J. Applied Physiol., 58: 1511-1516.
33.
Puvadolpirod, S. and J.P. Thaxton, 2000. Model of physiological stress in chicken. 1. Response parameters. Poult. Sci., 79: 363-369.
34.
Rosales, A.G., P. Villegas, P.D. Lukert, O.J. Fletcher, M.A. Mohamed and J. Brown, 1989. Isolation, identification and pathogenicity of two field strains of infectious bursal disease virus. Avian Dis., 33: 35-41.
35.
SAS., 2008. SAS Proprietary Software, Release 9.2. SAS Institute Inc., Cary, NC., USA.
36.
Selim, S.A., K.M. Gaafar and S.S. El-Ballal, 2012. Influence of in-ovo administration with vitamin E and ascorbic acid on the performance of Muscovy ducks. Emirates J. Food Agric., 24: 264-271.
37.
Szember, M., D. Ciosek and J. Pietrzyk, 1976. Serological identification of Escherichia coli strains isolated from monkeys affected with colibacillosis. Bul. Vet. Inst., 20: 61-64.
38.
Thaxton, P. and H.S. Siegel, 1970. Immunodepression in young chickens by high environmental temperature. Poult. Sci., 49: 202-205.
39.
Thaxton, P., C.R. Sadler and B. Glick, 1968. Immune response of chickens following heat exposure or injections with ACTH. Poult. Sci., 47: 264-266.
40.
Thornton, P.A., 1961. Increased environmental temperature influences on ascorbic acid activity in the domestic fowl. Feed. Proc., 20: 210-210.
41.
Vasconcelos, A.C., L. Moro and P.M. da Silva, 1996. Apoptose ou morte celular programada e sua importancia em patologia veterinaria. Rev. Port. Cienc. Vet., 91: 182-193.
42.
Wyatt, C.L., W.D. Weaver Jr., W.L. Beane and D.M. Denbow, 1986. Influence of hatcher holding times on several physiological parameters associated with the immune system of chickens. Poult. Sci., 65: 2156-2164.
43.
Yahav, S., R.S. Rath and D. Shinder, 2004. The effect of thermal manipulations during embryogenesis of broiler chicks (Gallus domesticus) on hatchability, body weight and thermoregulation after hatch. J. Therm. Biol., 29: 245-250.
44.
Yalcin, S., N. Bagdatlioglu, V. Bruggeman, E. Babacanoglu and I. Uysal et al., 2008. Acclimation to heat during incubation: 2. Embryo composition and residual egg yolk sac fatty acid profiles in chicks. Poult. Sci., 87: 1229-1236.
45.
Yalcm, S., S. Ozkan, M. Cabuk, J. Buyse, E. Decuypere and P.B. Siegel, 2005. Pre-and postnatal conditioning induced thermotolerance on body weight, physiological responses and relative asymmetry of broilers originating from young and old breeder flocks. Poult. Sci., 84: 967-976.
