Research Article Open Access

Experimental Study of Renal Tubular Cells Apoptosis Subsequent to Infectious by Influenza Virus (H9N2) in SPF Chickens

Authors

CORRESPONDING AUTHOR
Yousef Doustar
Department of Pathobiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran
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Daryoush Mohajeri
Department of Pathobiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran
|
Younes Anzabi
Department of Microbiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran
|
Mehrdad Nazeri
Faculty of Veterinary Medicine, Tabriz Branch, Islamic Azad University, Tabriz, Iran
Published on: October 15, 2011
DOI 10.3923/ijps.2011.891.894
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Abstract

Influenza virus produces cell death in animals and human. Since cell death can be caused by either necrosis or apoptosis. We investigated the types of cell death that occur in chickens infected with avian influenza virus, A/chicken/Iran/772/2000(H9N2). In experimental study 60 SPF chickens at 3 weeks old were divided to two groups. The first group was infected with 107.5 EID50 titer of the virus intranasaly and the second group was treated with saline normal. Following 72 hrs, renal tissues were collected and fixed in 10% formalin solution. The prepared microscopic sections with the thickness of 5-6 micron were stained using TUNEL method. In comparison to the control group, there were significant mean difference of apoptotic cells in renal tubular cells of the infected group (p<0.005). We demonstrated that A/chicken/Iran/772/2000 (H9N2) is able to induce apoptosis in renal tubular cells.

Keywords:

Apoptosis, avian influenza

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Copyright (c) 2011 Asian Network for Scientific Information

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This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

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How to Cite

Doustar , Y., Mohajeri, D., Anzabi, Y., & Nazeri, M. (2011). Experimental Study of Renal Tubular Cells Apoptosis Subsequent to Infectious by Influenza Virus (H9N2) in SPF Chickens. International Journal of Poultry Science, 10(11), 891–894. https://doi.org/10.3923/ijps.2011.891.894
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