Research Article
Open Access
Determination and Withdrawal Time of Fosfomycin in Chicken Muscle, Liver and Kidney
Abstract
A HPLC-MS/MS method was developed for the determination of fosfomycin in chicken muscle, liver and kidney. Organ samples were dispersed in silica gel with a subsequent clean up and elusion in a vacuum chamber followed by filtration with activated carbon to eliminate matrix components which are prone to produce unwanted matrix effects. Response was linear and recovery was determined to be between 81-106% for muscle, 92-102% for liver and 99-107% for kidney. The method developed is suitable to be used in withdrawal time studies. The withdrawal time of fosfomycin in broiler chickens, considering a MRL of 0.5 μg/g was studied. Twenty-four broiler chickens were assigned to two groups; in group one, fosfomycin was orally administered daily with 40 mg/kg bw and to the other group a dose of 10 mg/kg bw of the antibiotic was intramuscularly administered. Broilers were slaughtered 24, 48, 72 and 96 h later of oral treatment withdrawn and post intramuscular treatment. Although the longer WDT was of 2.55 days, a WDT of 3 days could be assigned as a precautionary principle for public health, without a significant economic impact for the broiler producer.
Keywords:
Chicken tissues, fosfomycin, HPLC MS-MS, maximum residues limit, withdrawal timeArticle Metrics
422
Abstract Views
217
File Views
0
Downloads
License
Copyright (c) 2011 Asian Network for Scientific Information
This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
Article Citation Metrics
How to Cite
Perez, D., Soraci, A., Dieguez, S., & Tapia, M. (2011). Determination and Withdrawal Time of Fosfomycin in Chicken Muscle, Liver and Kidney. International Journal of Poultry Science, 10(8), 644–655. https://doi.org/10.3923/ijps.2011.644.655
References
1.
Aramayona, J.J., M.A. Bregante, C. Solans, S. Rueda, L.J. Fraile and M.A. Garcia, 1997. Pharmacokinetics of fosfomycin in chickens after a single intravenous dose and tissue levels following chronic oral administration. Vet. Res., 28: 581-588.
2.
Borsa, F., A. Leroy, J.P. Fillastre, M. Godin and B. Moulin, 1988. Comparative pharmacokinetics of tromethamine fosfomycin and calcium fosfomycin in young and elderly adults. Antimicrob. Agents Chemother., 32: 938-941.
3.
Carraminana, J.J., C. Rota, I. Agustin and A. Herrera, 2004. High prevalence of multiple resistance to antibiotics in Salmonella serovars isolated from a poultry slaughterhouse in Spain. Vet. Microbiol., 104: 133-139.
4.
Clemente, M., M.P. Hermo, D. Barron and J. Barbosa, 2006. Confirmatory and quantitative analysis using experimental design for the extraction and liquid chromatography-UV, liquid chromatographymass spectrometry and liquid chromatography-mass spectrometry/mass spectrometry determination of quinolones in turkey muscle. J. Chrom. A, 35: 170-178.
5.
Dieguez, S., A. Soraci, O. Tapia, R. Carciochi, D. Perez, R. Harkes and O. Romano, 2011. Determination of antibiotic fosfomycin in chicken serum by liquid chromatography-tandem mass spectrometry. J. Liq. Chrom. Relat. Technol., 34: 116-128.
6.
Dios-Vieitez, M.C., M.M. Goni, M.J. Renedo and D. Fos, 1996. Determination of fosfomycin in human urine by capillary gas chromatography: Application to clinical pharmacokinetic studies. Chromatographia, 43: 293-295.
7.
Ellis, R., 2004. USFDA regulatory approach for control of residues of veterinary drugs. Joint FAO/WHO Technical Workshop on Residues of Veterinary Drugs Without ADI/MRL, Food and Agriculture Organization and World Health Organization, Geneva, Switzerland.
8.
EMEA, 1995. Note for Guidance: Approach towards harmonization of withdrawal periods. EMEA/CVMP/036/95, pp: 1-37. http://www.ema.europa.eu/docs/en_GB/document_library/Scientific_guideline/2009/10/WC500004428.pdf.
9.
EMEA, 2002. The European agency for the evaluation of medicinal products: Enrofloxacin, extension to all food producing species. Summary Report (5), EMEA/MRL/820/02-FINAL. http://www.ema.europa.eu/docs/en_GB/document_library/Maximum_Residue_Limits_-_Report/2009/11/WC500014151.pdf.
10.
European Commission Decision, 2002. Commission decision 2002/657/EC implementing council directive 96/23/EC concerning the performance of analytical methods and the interpretation of results. Official J. Eur. Commun., L221: 8-36.
11.
European Council Directive, 1999. Commission Regulation (EC) No 508/1999 of 4 March 1999 amending Annexes I to IV to Council Regulation (EEC) No 2377/90 laying down a community procedure for the establishment of maximum residue limits of veterinary medicinal products in foodstuffs of animal origin. Official J. Eur. Commun., L60: 16-52.
12.
European Council Directive, 2007. Council Directive 2007/43/EC laying down minimum rules for the protection of chickens kept for meat production. Official J. Eur. Commun., L182: 19-28.
13.
Falagas, M.E., K.P. Giannopoulou, G.N. Kokolakis and I.P. Rafailidis, 2008. Fosfomycin: Use beyond urinary tract and gastrointestinal infections. Clin. Infect. Dis., 46: 1069-1077.
14.
Fernandez, A., C. Lara, A. Loste, S. Calvo and M.C. Marca, 2001. Control of Salmonella enteritidis phage type 4 experimental infection by fosfomycin in newly hatched chicks. Comp. Immunol., Microbiol. Infect. Dis., 24: 207-216.
15.
Fernandez, A., C. Lara, A. Loste and M.C. Marca, 2002. Efficacy of calcium fosfomycin for the treatment of experimental infection of broiler chickens with Escherichia coli O78: K80. Vet. Res. Commun., 26: 427-436.
16.
Fernandez, L.C., E.L. Marino and A. Dominguez-Gil, 1987. Phosphomycin levels in serum and interstitial tissue fluid in a multiple dosage regimen in rabbits. Arzneimittelforschung, 37: 927-929.
17.
Fernandez, L.C., E.L. Marino and A. Dominguez-Gil, 1986. Linearity of the pharmacokinetics of phosphomycin in serum and interstitial tissue fluid in rabbits. Arzneimittelforschung, 36: 1518-1520.
18.
Gallego, A., A. Rodriguez and J.M. Mata, 1974. Fosfomycin: Pharmacological studies. Drugs Today, 10: 161-168.
19.
Gobernado, M., 2003. Fosfomicina. Rev. Esp. Quimioterapia Marzo., 16: 15-40.
20.
Grassi, G.G., 1990. Fosfomycin trometamol: Historical background and clinical development. Infection, 18: S57-S59.
21.
Gutierrez, O.L., C.L. Ocampo, J.R. Aguilera, J. Luna and L.H. Sumano, 2008. Pharmacokinetics of disodium-fosfomycin in mongrel dogs. Res. Vet. Sci., 85: 156-161.
22.
Valencia, T.M.G. and M.P.G. Camacho, 2010. On-line coupling of Matrix Solid Phase Dispersion (MSPD) technique and liquid chromatography for the determination of organophosphorus pesticides in biological tissues. Proceedings of the 1st International Congress on Instrumentation and Applied Sciences, October 26-29, 2010, Mexico, pp: 1-9.
23.
Hermo, M.P., D. Barron and J. Barbosa, 2006. Development of analytical methods for multiresidues determination of quinolones in pig muscle samples by liquid chromatography with ultraviolet detection, liquid chromatography-mass spectrometry and liquid chromatography tandem mass spectrometry. J. Chrom. A, 1104: 132-139.
24.
Hernandez, E., A. Loste, M.A. Bregante, M.A. Garcia and C. Solans, 2001. Determination of fosfomycin in chicken plasma samples by gas chromatography: Application to pharmacokinetic studies. Chromatographia, 54: 365-368.
25.
Kahan, F.M., J.S. Kahan, P.J. Cassidy and H. Kropp, 1974. The mechanism of action of fosfomycin (phosphonomycin). Ann. N.Y. Acad. Sci., 235: 364-386.
26.
Aramayona, J.J., M.A. Bregante, C. Solans, S. Rueda, L.J. Fraile and M.A. Garcia, 1997. Pharmacokinetics of fosfomycin in chickens after a single intravenous dose and tissue levels following chronic oral administration. Vet. Res., 28: 581-588.
27.
KuKanich, B., R. Gehring, A.L. Webb, A.L. Craigmill and J.E. Riviere, 2005. Effect of formulation and route of administration on tissue residues and withdrawal times. Am. Vet. Med. Ass., 227: 1574-1577.
28.
Goyal, S.S., A.A.R. Hafez and D.W. Rains, 1993. Simultaneous determination of total sodium, potassium, magnesium and calcium in plant tissues using acid digestion and ion chromatography. Agron. J., 85: 1192-1197.
29.
Li, L., X. Chen, X. Dai, M.H. Chen and D. Zhong, 2007. Rapid and selective liquid chormatographic/tandem mass spectrometric method for the determination of fosfomycin in human plasma. J. Chrom. B., 856: 171-177.
30.
Lin, E.C.C., 1976. Glycerol dissimilation and its regulation in bacteria. Ann. Rev. Microbiol., 30: 535-578.
31.
Lopez, M.I., J.S. Pettis, I.B. Smith and P.S. Chu, 2008. Multiclass determination and confirmation of antibiotic residues in Money using LC-MS/MS. J. Agric. Food Chem., 56: 1553-1559.
32.
Loste, A., E. Hernandez, M.A. Bregante, M.A. Garcia and C. Solans, 2002. Development and validation of a gas chromatographic method for analysis of fosfomycin in chicken muscle samples. Chromatografia, 56: 181-184.
33.
Mata, J., A. Rodriguez and A. Gallego, 1977. Fosfomycin: In vitro activity. Chemother, 23: 23-24.
34.
Matuszewski, B.K., M.L. Constanzer and C.M. Chavez-Eng, 1998. LC/MS/MS analyses of biological fluids: A method for determination of finasteride in human plasma at pictogram per milliliter concentrations. Anal. Chem., 70: 882-889.
35.
Patel, S.S., J.A. Balfour and H.M. Bryson, 1997. Fosfomycin tromethamine. A review of its antibacterial activity, pharmacokinetic properties and therapeutic efficacy as a single-dose oral treatment for acute uncomplicated lower urinary tract infections. Drugs, 53: 637-656.
36.
Petsch, M., B.X. Mayer-Helm, R. Sauermann, C. Joukhadar and E. Kenndler, 2005. Determination of fosfomycin in pus by capillary zone electrophoresis. J. Chrom. A, 1081: 55-59.
37.
Popovic, M., D. Steinort, S. Pillai and C. Joukhadar, 2010. Fosfomycin: An old, new friend?. Eur. J. Clin. Microbiol. Infect. Dis., 29: 127-142.
38.
Riviere, J.E., A.I. Webb and A.L. Craigmill, 1998. Primer on estimating withdrawal times after extralabel drug use. J. Am. Vet. Med. Assoc., 213: 966-969.
39.
San Martin, B., J. Cornejo, D. Iraguen, H. Hidalgo and A. Anadon, 2007. Depletion study of enrolfoxacin and its metabolite ciprofloxacin in edible tissues and feathers of white leghorn hens by liquid chromatography coupled with tandem mass spectrometry. J. Food Prot., 70: 1952-1957.
40.
Segre, G., E. Bianchi, A. Cataldi and G. Zannini, 1987. Pharmacokinetic profile of fosfomycin trometamol (Monuril). Eur. Urol., 13: 56-63.
41.
Soraci, A.L., D.S. Perez, G. Martinez, S. Dieguez and M.O. Tapia et al., 2011. Disodium-fosfomycin pharmacokinetics and bioavailability in post weaning piglets. Res. Vet. Sci., 90: 498-502.
42.
Sumano, L.H., C.L. Ocampo and O.L. Gutierrez, 2007. Intravenous and intramuscular pharmacokinetics of a single-daily dose of disodium-fosfomycin in cattle, administered for 3 days. J. Vet. Pharmacol. Ther., 30: 49-54.
43.
Tang, H.P., C. Ho and S.S. Lai, 2006. High-troughput screening for multi-class veterinary drug residues in animal muscle using liquid chromatography/tandem mass spectrometry with on-line solid-phase extraction. Rapid Commun. Mass Spectrom., 20: 2565-2572.
44.
Tzanavaras, P.D. and D.G. Themelis, 2002. Flow injection spectrophotometric determination of the antibiotic fosfomycin in pharmaceutical products and urine samples after on-line thermal-induced digestion. Anal. Biochem., 304: 244-248.
45.
USFDA, 2001. Guidance for Industry: Bioanalytical Method Validation. US Department of Health and Human Services, Rockville, MD.
46.
Hu, Y.L., Y.Q. Fen, P.H. Zhang and S.L. Da, 1999. Determination of fosfomycin by indirect spectrophotometric method. Talanta, 49: 47-52.
47.
Zozaya, D.H., O.L. Gutierrez, C.L. Ocampo and L.H. Sumano, 2008. Pharmacokinetics of a single bolus intravenous, intramuscular and subcutaneous dose of disodium fosfomicina in horses. J. Vet. Pharmacol. Ther., 31: 321-327.
