Research Article
Open Access
Sequence Analysis of HN and F Genes of a less Virulent Newcastle Disease Virus Isolated from Unvaccinated Village Chicken
Abstract
The HN and F genes of the D58 isolate have coding senquence of 577 and 553 amino acids respectively. The HN gene has stalk (1-143) and globular head (123-571) regions with transmembrane domain at positions 25-45, sialic acid residue at 234-239, glycosylation sites at 119, 341, 433, 481 and 538 and cysteine residues at 172, 186, 196, 238, 247, 251, 344, 455, 461, 465, 531 and 542. The glycosylation site at 508 specific for virulent strains and cysteine residue at 123 were absent in D58 isolate. The F gene has transmembrane domains at positions 15-25 and 501-523 and glycosylation sites at 85, 191, 366, 447, 471 and 541. The fusion protein cleavage site has amino acids GRQGRL and fusion peptide starts with leucine. The HN and F genes have 26 and 23 B cell epitopes respectively. The D58 isolate was grouped with less virulent strains in phylogenetic analysis.
Keywords:
Avian paramyxovirus 1, B cell epitopes, F gene, HN gene, newcastle disease, phylogenetic analysis, virulenceArticle Metrics
109
Abstract Views
111
File Views
0
Downloads
License
Copyright (c) 2009 Asian Network for Scientific Information
This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
Article Citation Metrics
How to Cite
Ponnusamy, P., Kirubaharan, J. J., & Chandramohan, A. (2009). Sequence Analysis of HN and F Genes of a less Virulent Newcastle Disease Virus Isolated from Unvaccinated Village Chicken. International Journal of Poultry Science, 8(10), 985–994. https://doi.org/10.3923/ijps.2009.985.994
References
1.
Aldous, E.W. and D.J. Alexander, 2001. Detection and differentiation of Newcastle disease virus (avian paramyxovirus type 1). Avian Pathol., 30: 117-128.
2.
Alexander, D.J., 2003. Newcastle Disease, Other Paramyxo Viruses and Pneumovirus Infections. In: Diseases of Poultry, Saif, Y.M., H.J. Barnes, J.R. Ghison, A.M. Fadly, Mc Douglad and D.E. Sweyne (Eds.). 11th Edn., Iowa State Press, Amcs IA, pp: 63-100.
3.
Ananth, R., J. John-Kirubaharan, M.L.M. Priyadarshini and A. Albert, 2008. Isolation of newcastle disease viruses of high virulence in unvaccinated healthy village chickens in South India. Int. J. Poult. Sci., 7: 368-373.
4.
Bates, P.A. and M.J. Sternberg, 1999. Model building by comparison at CASP3: Using expert knowledge and computer automation. Proteins, 3: 47-54.
5.
Bates, P.A., L.A. Kelley, R.M. MacCallum and M.J.E. Sternberg, 2001. Enhancement of protein modeling by human intervention in applying the automatic programs 3D-JIGSAW and 3D-PSSM. Proteins, 5: 39-46.
6.
Buckland, R., E. Malvoisin and P. Beauverger, 1992. A leucine zipper structure present in the measles virus fusion protein is not required for its tetramerization but is essential for fusion. J. General Virol., 73: 1703-1707.
7.
Contreras-Moreira, B. and P.A. Bates, 2002. Domain fishing: A first step in protein comparative modelling. Bioinformatics, 18: 1141-1142.
8.
Cserzo, M., E. Wallin, I. Simon, G. von Heijne and A. Elofsson, 1997. Prediction of transmembrane alpha-helices in procariotic membrane proteins: The dense alignment surface method. Prot. Eng., 10: 673-676.
9.
Deng, R., A.M. Mirza, P.J. Mahon and R.M. Iorio, 1997. Functional chimeric HN glycoproteins derived from Newcastle disease virus and human parainfluenza virus-3. Arch. Virol. Suppl., 13: 115-130.
10.
Doyle, T.M., 1927. A hitherto unrelated disease of fowls due to a filter passing virus. J. Comp. Pathol., 40: 144-144.
11.
Crennell, S., T. Takimoto, A. Portner and G. Taylor, 2000. Crystal structure of the multifunctional Paramyxovirus hemagglutinin-neuraminidase. Nat. Struct. Biol., 7: 1068-1074.
12.
Gravel, A.K. and T.G. Morrison, 2003. Interacting domains of HN and F genes of Newcastle disease viruses. J. Virol., 77: 11040-11049.
13.
Huang, Z., S. Krishnamurthy, A. Panda and S.K. Samal, 2003. Newcastle disease virus V protein is associated with viral pathogenesis and functions as an alpha interferon antagonist. J. Virol., 77: 8676-8685.
14.
Iorio, R.M., R.J. Syddall, R.L. Glickman, A.M. Riel, J.P. Sheehan and M.A. Bratt, 1989. Identification of amino acid residues important to the neuraminidase activity of the HN glycoprotein of Newcastle disease virus. Virology, 156: 12-14.
15.
Lamb and D. Kolakofsky, 1996. Paramyxoviridae the Viruses and Their Replication. In: Fields Virology, Fields, B.N., D.N. Knife and P.M. Howley (Eds.). 3rd Edn., Lippincott-Raven, Philadelphia, pp: 1177-1203.
16.
Lei-Tao, T., X. Huai-Ying, W. You-Ling, Q. Zhuo-Ming, L. Sun and C. Zhi-Zhong, 2008. Molecular characterization of 3 new virulent Newcastle disease virus variants isolated in China. J. Clin. Microbiol., 46: 750-753.
17.
Yu, L., Z. Wang, Y. Jiang, L. Chang and J. Kwank, 2001. Characterization of newly emerging Newcastle disease virus isolates from the People's Republic of China and Taiwan. J. Clin. Microbiol., 39: 3512-3519.
18.
McGinnes, L.W., K. Gravel and T.G. Morrison, 2002. Newcastle disease virus HN protein alters the conformation of the F protein at cell surfaces. J. Virol., 76: 12622-12633.
19.
Romer-Oberdorfer, A., O. Werner, J. Veits, T. Mebatsion and T.C. Mettenleiter, 2003. Contribution of the length of the HN protein and the sequence of the F protein cleavage site to Newcastle disease virus pathogenicity. J. General Virol., 84: 3121-3129.
20.
OIE., 2004. Manual of Diagnostic tests and Vaccines for Terrestrial Animals 2004. 5th Edn., OIE Publications, UK.
21.
Olav, S., O.S. Deleeuw, G.L. Hantog, N. Ravenshost and B.P. Peeters, 2005. Virulence of Newcastle disease virus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin-neuraminidase protein. J. Gen. Virol., 86: 1759-1769.
22.
Peeters, B.P., O.S. Deleeuw, I. Verstigen, G. Koch and A.L. Gielkens, 2001. Generation of a recombinant chimeric Newcastle disease virus vaccine that allows serological differentiation between vaccinated and infected animals. Vaccine, 19: 1616-1627.
23.
Pitt, J.J., E. da Silva and J. Gorman, 2000. Determination of the disulfide bond arrangement of Newcastle disease virus Haemagglutinin-neuraminidase Correlation with a beta-sheet propeller structural fold predicted for Paramyxoviridae attachment proteins. J. Biol. Chem., 275: 6469-6478.
24.
Reitter, J., T. Sergel and T.G. Morrison, 1995. Mutational analysis of the leucine zipper motif in the Newcastle disease virus fusion protein. J. Virol., 69: 5995-6004.
25.
Sakaguchi, M., H. Nakamura, K. Sonoda, F. Hamada and K. Hirai, 1996. Protection of chickens from Newcastle disease by vaccination with a linear plasmid DNA expressing the F protein of Newcastle disease virus. Vaccine, 14: 747-752.
26.
Sakaguchi, T., T. Toyoda, B. Gotoh, N.M. Inocencio, K. Kuma, T. Miyata and Y. Nagai, 1989. Newcastle disease virus evolution I Multiple lineages defined by sequence variability of the hemagglutinin-neuraminidase gene. Virology, 169: 260-272.
27.
Scheid, A. and P.W. Choppin, 1974. The haemagglutinin and neuraminidase protein of a paramyxovirus: Interaction with neuramic acid in affinity chromatography. Virology, 62: 125-133.
28.
Tan, W.S., C.H. Lau, B.K. Ng, A.L. Ibrahim and K. Yusoff, 1995. Nucleotide sequence of the haemagglutinin-neuraminidase (HN) gene of a Malaysian heat resistant viscerotropic-velogenic Newcastle disease virus. DNA Sequencing, 6: 47-50.
29.
Yusoff, K. and W.S. Tan, 2001. Newcastle disease virus macromolecules and opportunities. Avian Pathol., 30: 439-455.
