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Effects of Polyphenols Extracted from Tamarind (Tamarindus indica L.) Seed Coat on Differential White Blood Cell Count in Broilers (Gallus domesticus) Exposed to High Environmental Temperature
Abstract
Tamarind (Tamarindus indica L.) is a plant that belongs to the Leguminosae family and grows naturally in tropical and subtropical regions. Tamarind seed coat is composed of polyphenols especially procyanidin oligomers. The objective of this experiment was to determine the effect of polyphenols extracted from tamarind seed coat on differential white blood cell counts of broilers maintained at high environmental temperature. Broilers were divided into 2 groups. In group 1, broilers were maintained in environmental temperature at 26±2oC throughout experimental period, and in group 2, broilers were maintained in environmental temperature at 38±2oC and received polyphenols i.e. 0, 100, 200, 300, 400 and 500 mg/kg in diets. Differential white blood cell counts were investigated on days 1, 7, 14 and 21 of experimental period. The results revealed the following information: Lymphocyte and basophil levels of broilers maintained in the environmental temperature at 38±2oC and received polyphenols at 400 mg/kg in diet were increased (p<0.05). On the other hand, the heterophil and monocyte levels of broilers maintained in the environmental temperature at 38±2oC and received polyphenols at 400 mg/kg in diet were decreased (p<0.05). This occurrence indicated that polyphenols extracted from tamarind seed coat could reduce heat stress in broilers.
Keywords:
Basophil, eosinophil, heat stress, heterophil, lymphocyte, monocyteArticle Metrics
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Copyright (c) 2009 Asian Network for Scientific Information
This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
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Aengwanich, W., Suttajit, M., & Narkkong, N. (2009). Effects of Polyphenols Extracted from Tamarind (Tamarindus indica L.) Seed Coat on Differential White Blood Cell Count in Broilers (Gallus domesticus) Exposed to High Environmental Temperature. International Journal of Poultry Science, 8(10), 957–962. https://doi.org/10.3923/ijps.2009.957.962
References
1.
Altan, O., A. Altan, M. Cabuk and H. Bayraktar, 2000. Effects of heat stress on some blood parameters in broilers. Turk. J. Vet. Anim. Sci., 24: 145-148.
2.
Beker, A. and R.G. Teeter, 1994. Drinking water temperature and potassium chloride supplementation effect on broiler body temperature and performance during heat stress. J. Applied Poult. Res., 3: 87-92.
3.
Borges, S.A., J. Ariki, C.L. Martins and V.B.M. de Moraes, 1999. Potassium chloride supplementation in heat stressed broilers. Rev. Bras. de Zootec., 28: 313-319.
4.
Borges, S.A., A.V.F. da Silva, J. Ariki, D.M. Hooge and K.R. Cummings, 2003. Dietary electrolyte balance for broiler chickens exposed to thermoneutral or heat-stress environments. Poult. Sci., 82: 428-435.
5.
Borges, S.A., A.V.F. da Silva, A. Majorka, D.M. Hooge and K.R. Cummings, 2004. Physiological response of broiler chickens to heat stress and dietary electrolyte balance (sodium plus potassium minus chloride, milliequivalents perkilogram). Poult. Sci., 83: 1551-1558.
6.
Bush, B.M., 1991. Interpretation of Laboratory Results for Small Animal Clinicians. Backwell Scientific Publications, London, ISBN: 9780408108492, Pages: 515.
7.
Campo, J.L. and S.G. Davila, 2002. Changes in heterophil to lymphocyte ratios of heat-stressed chickens in response to dietary supplementation of several related stress agents. Arch. Geflugelk, 66: 80-84.
8.
Cooper, M.A. and K.W. Washburn, 1998. The relationships of body temperature to weight gain, feed consumption and feed utilization in broilers under heat stress. Poult. Sci., 77: 237-242.
9.
Daskiran, M., R.G. Teeter, S.L. Vanhooser, M.L. Gibson and E. Roura, 2004. Effect of dietary acidification on mortality rates, general performance, carcass characteristics and serum chemistry of broilers exposed to cycling high ambient temperature stress. J. Applied Poult. Res., 13: 605-613.
10.
Gu, L., M.A. Kelm, J.F. Hammerstone, Z. Zhang and G. Beecher et al., 2003. Liquid chromatographic/electrospray ionization mass spectrometric studies of procyanidins in foods. J. Mass Spectrometry, 38: 1272-1280.
11.
Jain, N.C., 1993. Essentials of Veterinary Hematology. 1st Edn., Lea and Febiger Publication, Philadelphia, ISBN: 9780812114379, Pages: 417.
12.
Komutarin, T., S. Azadi, L. Butterworth, D. Keil, B. Chitsomboon, M. Suttajit and B.J. Meade, 2004. Extract of the seed coat of Tamarindus indica inhibits nitric oxide production by murine macrophages in vitro and in vivo. Food Chem. Toxicol., 42: 649-658.
13.
Lien, R.J., J.B. Hess, S.R. McKee, S.F. Bilgili and J.C. Townsend, 2007. Effect of light intensity and photoperiod on live performance, heterophil-to-lymphocyte ratio and processing yields of broilers. Poult. Sci., 86: 1287-1293.
14.
Lohakare, J.D., M.H. Ryu, T.W. Hahn, J.K. Lee and B.J. Chae, 2005. Effects of supplementation ascorbic acid on the performance and immunity of commercial broilers. J. Applied Poult. Res., 14: 10-19.
15.
Mashaly, M.M., G.L. Hendricks, M.A. Kalama, A.E. Gehad, A.O. Abbas and P.H. Patterson, 2004. Effect of heat stress on production parameters and immune responses of commercial laying hens. Poult. Sci., 83: 889-894.
16.
Martinello, F., S.M. Soares, J.J. Franco, A.C. Santos and A. Sugohara et al., 2006. Hypolipemic and antioxidant activities from Tamarindus indica L. pulp fruit extract in hypercholesterolemic hamsters. Food Chem. Toxicol., 44: 810-818.
17.
Maxwell, M.H., G.W. Robertson, M.A. Mitchell and A.J. Carlisle, 1992. The fine structure of broiler chicken blood cells, with particular reference to basophils, after severe heat stress. Comp. Haematol. Int., 2: 190-200.
18.
McKee, J.S., P.C. Harrison and G.L. Riskowski, 1997. Effects of supplemental ascorbic acid on the energy conversion of broiler chicks during heat stress and feed withdrawal. Poult. Sci., 76: 1278-1286.
19.
Mujahid, A., Y. Yoshiki, Y. Akiba and M. Toyomizu, 2005. Superoxide radical production in chicken skeletal muscle induced by acute heat stress. Poult. Sci., 84: 307-314.
20.
Naseem, S., M. Younus, B. Anwar, A. Ghafoor, A. Aslam and S. Akhter, 2005. Effect of ascorbic acid and acetylsalicylic acid supplementation on performance of broiler chicks exposed to heat stress. Int. J. Poult. Sci., 4: 900-904.
21.
Post, J., M.J. Rebel and A.A.H.M. Ter-Huurne, 2003. Physiological effects of evaluated plasma corticosterone concentrations in broiler chickens. An alternative means by which to assess the physiological effects of stress. Poult. Sci., 82: 1313-1318.
22.
Pumthong, G., 1999. Antioxidative Activity of Polyphenolic Compounds Extracted from Seed Coat of Tamarindus indicus Linn. Chiangmai Mai University, Thaialnd.
23.
Puron, D., R. Santamaria and J.C. Segura, 1994. Effects of sodium bicarbonate, acetylsalicylic and ascorbic acid on broiler performance in a tropical environment. J. Applied Poult. Sci., 3: 141-145.
24.
Puron, D., R. Santamaria and J.C. Segura, 1997. Sodium bicarbonate and broiler performance at high stocking densities in a tropical environment. J. Applied Poult. Res., 6: 443-448.
25.
Puthpongsiriporn, U., S.E. Scheideler, J.L. Sell and M.M. Beck, 2001. Effects of vitamin E and C supplementation on performance, In vitro lymphocyte proliferation and antioxidant status of laying hens during heat stress. Poult. Sci., 80: 1190-1200.
26.
Ribeiro, A.M.L. and A.M. Penz Jr., 2001. Effects of 2-Hydroxy-4-(methylthio)butanoic acid and dl-methionine on broiler performance and compensatory growth after exposure to two different environmental temperatures. J. Applied Poult. Res., 10: 419-426.
27.
Ritchie, B.W., G.J. Harrison and R.L. Harrison, 1994. Avian Medicine: Principle and Application. 6th Edn., Wingers Publishing, Inc., Florida.
28.
Roussan, D.A., G.Y. Khwaldeh, R.R. Haddad, I.A. Shaheen, G. Salameh and R. Al-Rifai, 2008. Sodium bicarbonate and potassium chloride supplementation in water on the performance of broiler chickens exposed to heat stress. J. Applied Poult. Res., 17: 141-144.
29.
Sands, J.S. and M.O. Smith, 1999. Broilers in heat stress conditions: Effects of dietary manganese proteinate or chromium picolinate supplementation. J. Applied Poult. Res., 8: 280-287.
30.
Smith, M.O. and R.G. Teeter, 1992. Effects of potassium chloride supplementation on growth of heat-distressed broilers. J. Applied Poult. Res., 1: 321-324.
31.
Smith, M.O. and R.G. Teeter, 1993. Carbon dioxide, ammonium chloride, potassium chloride and performance of heat distressed broiler. J. Applied Poult. Res., 2: 61-66.
32.
Tsuda, T., M. Watanabe, K. Ohshima, A. Yamamoto, S. Kawakishi and T. Osawa, 1994. Antioxidative components isolated from the seed of tamarind (Tamarindus indica L.). J. Agric. Food Chem., 42: 2671-2674.
33.
Tsuda, T., K. Mizono, K. Ohshima, S. Kawakishi and T. Osawa 1995. Supercritical carbon dioxide extraction of antioxidative component from tamarind (Tamarindus indica L.) seed coat. J. Agric. Food Chem., 43: 2803-2806.
34.
Yalcin, S., S. Ozkan, G. Oktay, M. Cabuk, Z. Erbayraktar and S.F. Bilgili, 2004. Age-related effects of catching, crating and transportation at different season on core body temperature and physiological blood parameters in broilers. J. Applied Poult. Res., 13: 549-560.
35.
Zulkifli, I., M.T.C. Norma, D.A. Israf and A.R. Omar, 2000. The effect of early age feed restriction on subsequent response to high environmental temperatures in female broiler chickens. Poult. Sci., 78: 1401-1407.
36.
Kähkönen, M.P., A.I. Hopia, H.J. Vuorela, J.P. Rauha, K. Pihlaja, T.S. Kujala and M. Heinonen, 1999. Antioxidant activity of plant extracts containing phenolic compounds. J. Agric. Food Chem., 47: 3954-3962.
