Research Article Open Access

Expression Profile of Toll-Like Receptor mRNA in an Indigenous Aseel Breed of Chicken in India

Authors

CORRESPONDING AUTHOR
G. Dhinakar Raj
Department of Animal Biotechnology, Madras Veterinary College,Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 007, India
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T.M. Chozhavel Rajanathan
Department of Animal Biotechnology, Madras Veterinary College,Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 007, India
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K. Kumanan
Department of Animal Biotechnology, Madras Veterinary College,Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 007, India
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S. Elankumaran
Department of Animal Biotechnology, Madras Veterinary College,Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 007, India
Published on: June 15, 2009
DOI 10.3923/ijps.2009.651.655
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Abstract

Expression profile of chicken six Toll-like Receptor (TLR) mRNAs were analyzed in the heterophils, lungs, liver, spleen, duodenum, caecal tonsils and kidneys of 12-weeks old indigenous village chicken in India, using reverse transcription polymerase chain reaction (RT-PCR). TLR 2 type 1 mRNA was expressed in lungs, liver, spleen, duodenum and caecal tonsils. TLR2 type 2 mRNA was expressed only in the lungs. TLR 3 mRNA was expressed in lungs, liver, spleen and caecal tonsils. TLR 4 mRNA was expressed only in lungs, liver and spleen. TLR 5 and TLR 7 mRNAs were expressed in all the tissues examined. With respect to tissues, heterophils and lungs expressed all the TLR mRNAs examined while kidneys expressed only TLR 5 and TLR 7 mRNAs. All the TLR amplified PCR products were partially sequenced and showed high homologies with the available chicken (commercial) TLR gene sequences. There could be a possibility of correlation between TLR mRNA expressions with higher levels of innate immunity seen in indigenous village breeds of chickens.

Keywords:

Expression profiles, indigenous chicken, RT-PCR, toll-like receptors

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Copyright (c) 2009 Asian Network for Scientific Information

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This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

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How to Cite

Raj, G. D., Rajanathan, T. C., Kumanan, K., & Elankumaran, S. (2009). Expression Profile of Toll-Like Receptor mRNA in an Indigenous Aseel Breed of Chicken in India. International Journal of Poultry Science, 8(7), 651–655. https://doi.org/10.3923/ijps.2009.651.655
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