Research Article Open Access

Relation Between the SpvC and InvA Virulence Genes and Resistance of Salmonella enterica Serotype Enteritidis Isolated from Avian Material

Authors

CORRESPONDING AUTHOR
Adriano Sakai Okamoto
Department of Veterinary Clinics, FMVZ UNESP Campus, Botucatu, Brazil
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Raphael Lucio Andreatti Filho
Department of Veterinary Clinics, FMVZ UNESP Campus, Botucatu, Brazil
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Ticiana Silva Rocha
Department of Veterinary Clinics, FMVZ UNESP Campus, Botucatu, Brazil
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Anita Menconi
Department of Veterinary Clinics, FMVZ UNESP Campus, Botucatu, Brazil
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Guilherme Augusto Marietto-Goncalves
Department of Veterinary Clinics, FMVZ UNESP Campus, Botucatu, Brazil
Published on: May 15, 2009
DOI 10.3923/ijps.2009.579.582
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Abstract

Pathogenic bacteria possess genes responsible for virulence, that when expressed, determine not only bacterial invasion and persistence and destruction of host cells but also survival capacity under inappropriate culture conditions. This study utilized 100 samples of Salmonella enterica subspecies enterica Serotype Enteritidis (SE) isolated from avian material, detected the virulence genes InvA and SpvC by medium of Polymerase Chain Reaction (PCR) and compared them for possible expression of virulence factors for survival in hostile conditions of temperature, pH and reduced concentration of nutrients necessary for SE multiplication. Of all the samples analyzed, two presented the genes SpvC and InvA, simultaneously, with one probable expression of them being verified in growth with pH 10.0 or temperature of 25oC. But in relation to nutrient concentration, neither sample obtained growth when seeded in medium containing 0.5% nutrient.

Keywords:

Gene InvA, gene SpvC, resistance, Salmonella enteritidis, virulence

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Copyright (c) 2009 Asian Network for Scientific Information

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How to Cite

Okamoto, A. S., Filho, R. L. A., Rocha, T. S., Menconi, A., & Marietto-Goncalves, G. A. (2009). Relation Between the SpvC and InvA Virulence Genes and Resistance of Salmonella enterica Serotype Enteritidis Isolated from Avian Material. International Journal of Poultry Science, 8(6), 579–582. https://doi.org/10.3923/ijps.2009.579.582
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