Research Article Open Access

Study on Immunomodulatory Activity of Dietary Garlic in Chickens Vaccinated against Avian Influenza Virus (Subtype H9N2)

Authors

CORRESPONDING AUTHOR
R.A. Jafari
Division of Poultry Diseases, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran
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M. Ghorbanpoor
Division of Microbiology, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran
|
S. Hoshmand Diarjan
Division of Microbiology,Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran
Published on: March 15, 2009
DOI 10.3923/ijps.2009.401.403
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Abstract

Fresh garlic powder was evaluated for ability to potentiate the immune response of broiler chicks to Avian Influenza Virus (AIV) vaccine. For this purpose, 280 day-old chicks (Ross 308) were randomly allocated to 4 groups A, B (52 each) C and D (88 each). The birds in groups A and B were given control diet during the experiment, but those in groups C and D received diet supplemented with 1 and 3% garlic powder, respectively. After 2nd bleeding, half of the chicks in groups C and D were separated as groups E and F and fed control diet thereafter. On 9th day of age, the chicks in all groups except A were immunized subcutaneously against AIV (subtype H9N2) with a commercial oil-based inactivated vaccine (Merial, France). Fifteen chicks from each group were bled on days 14, 24 and 34 post vaccination and also 5 just before vaccination. The sera were used for antibody titration against AIV by both HI and ELISA tests. The results showed that antibody levels were considerably higher in the vaccinated chicks than those in the non-vaccinated control throughout the experimental period (p<0.05) but not affected by the treatment (p>0.05). In addition, the removal of garlic from diet had no significant (p>0.05) effect on serum titer. It is suggested that diet supplementation with garlic powder can not stimulate the humoral response of chickens against AIV vaccine.

Keywords:

Broiler, garlic, H9N2, humoral, immunity, immunomodulator

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Jafari, R., Ghorbanpoor, M., & Diarjan, S. H. (2009). Study on Immunomodulatory Activity of Dietary Garlic in Chickens Vaccinated against Avian Influenza Virus (Subtype H9N2). International Journal of Poultry Science, 8(4), 401–403. https://doi.org/10.3923/ijps.2009.401.403
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References

1.
Chinnah, A.D., M.A. Baig, I.R. Tizard and M.C. Kemp, 1992. Antigen dependent adjuvant activity of a polydispersed beta-(1,4)-linked acetylated mannan (acemannan). Vaccine, 10: 551-557.
2.
Chowdhury, S.R., S.D. Chowdhury and T.K. Smith, 2002. Effects of dietary garlic on cholesterol metabolism in laying hens. Poult. Sci., 81: 1856-1862.
3.
Corzo-Martinez, M., N. Corzo and M. Villamiel, 2007. Biological properties of onions and garlic. Trends Food Sci. Technol., 18: 609-625.
4.
Dorhoi, A., V. Dobrean, M. Zahan and P. Virag, 2006. Modulatory effects of several herbal extracts on avian peripheral blood cell immune responses. Phytother. Res., 20: 352-358.
5.
Gabor, S., P. Vilmos, N. Bela, E. Istvanne and N. Gyorgy et al., 1998. New type of immuno-stimulant to increase antibody production in response to viral and bacterial vaccines. Magyar Allatorvosok Lapja, 120: 719-721.
6.
Ghazanfari, T., Z. Hassan and M. Ebrahimi, 2002. Immunomodulatory activity of a protein isolated from garlic extract on delayed type hypersensitivity. Int. Immonpharmacol., 2: 1541-1549.
7.
Groom, S.N., T. Johns and P.R. Oldfield, 2007. The potency of immunomodulatory herbs may be primarily dependent upon macrophage activation. J. Med. Food, 10: 73-79.
8.
Jafari, R.A., M. Razi-Jalali, M. Ghorbanpoor and S.M.R.M. Saraei, 2008. Effect of dietary garlic on immune response of broiler chicks to live Newcastle disease vaccine. Pak. J. Biol. Sci., 11: 1848-1851.
9.
Kyo, E., N. Uda, S. Kasuga and Y. Itakura, 2001. Immunomodulatory effects of aged garlic extract. J. Nutr., 131: 1075S-1079S.
10.
Lanzotti, V., 2006. The analysis of onion and garlic. J. Chromatogr. A, 1112: 3-22.
11.
Lawson, L.D., 1996. The Composition and Chemistry of Garlic Cloves and Processed Garlic. In: Garlic: The Science and Therapeutic Application of Allium sativum L. and Related Species, Koch, H.P. and L.D. Lawson (Eds.). Williams and Wilkins, Boltimore, pp: 37-108.
12.
NRC, 1994. Nutrient Requirements of Poultry: Nutrient Requirements of Domestic Animals. 9th Edn., National Academy Press, Washington, DC., USA., pp: 155.
13.
OIE, 2008. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. 6th Edn., OIE, Paris, pp: 470-471.
14.
Patya, M., M.A. Zahalka, A. Vanichkin, A. Rabinkov and T. Miron et al., 2004. Allicin stimulates lymphocytes and elicits an anti-tumor effect: A possible role of p21ras. Int. Immunol., 16: 275-281.
15.
Petrie, A. and P. Watson, 2006. Statistics for Veterinary and Animal Science. 2nd Edn., Blackwell Publishing, USA., pp: 95-104.
16.
Sullivan, A.M., J.G. Laba, J.A. Moore and T.D. Lee, 2008. Echinacea-induced macrophage activation. Immunopharmacol. Immunotoxicol., 30: 553-574.
17.
Taheri, H.R., H.R. Rahmani and J. Pourreza, 2005. Humoral immunity of broilers is affected by oil extracted propolis (OEP) in the diet. Int. J. Poult. Sci., 4: 414-417.
18.
Tatara, M.R., E. Sliwa, K. Dudek, A. Gawron and T. Piersiak et al., 2008. Aged garlic extract and allicin improve performance and gastrointestinal tract development of piglets reared in artificial sow. Ann. Agric. Environ. Med., 15: 63-69.