Study on Molecular OPAY02-Scar Markers Related to Early Body Weight of New Yangzhou Chickens


Authors

  • G.J. Dai Animal Science and Technology College, Yangzhou University, Yangzhou China, 225009
  • J.Y. Wang Jiangsu Jinghai Broiler Grouping Company, Nantong Jiangsu, China, 226100
  • O. Olajide Adeyemi College of Education, P.M B. 520, Ondo, Nigeria
  • Q.S. Li Animal Science and Technology College, Yangzhou University, Yangzhou China, 225009
  • H. Shen Animal Science and Technology College, Yangzhou University, Yangzhou China, 225009
  • K.Z. Xie Animal Science and Technology College, Yangzhou University, Yangzhou China, 225009
  • Z.Y. Wang Animal Science and Technology College, Yangzhou University, Yangzhou China, 225009
  • S.L. Wu Animal Science and Technology College, Yangzhou University, Yangzhou China, 225009
  • Y.P. Gu Jiangsu Jinghai Broiler Grouping Company, Nantong Jiangsu, China, 226100
  • G.X. Zhang Animal Science and Technology College, Yangzhou University, Yangzhou China, 225009

DOI:

https://doi.org/10.3923/ijps.2005.683.688

Keywords:

Bioinformatics, DNA sequencing, genotype equilibrium test, OPAY02- SCAR genetic markers

Abstract

In order to develop rapid and reliable SCAR markers linked to the QTL, to get better understood of orgin of New Yangzhou chickens, two bands of OPAY02 type marker have been cloned, sequenced and two pairs of primer were designed according to the DNA sequence of two bands of OPAY02 to amplify the SCAR-markers on 86 individuals randomly selected from New Yangzhou chickens. The results of comparing the two bands DNA sequence with red jungle fowl genomic DNA database showed that the large molecular size band is located on the 3rd chromosome, the sequence identities were 98% and 8 SNPs mutations were detected, they were at 195 (T→G), 316 (A→T), 538 (G→ A), 731 (T→A), 1147 (G→A), 1329 (T→C), 1927 (C→ T) and 2081 (C→T). On the other hand, the small molecular size band can`t be found in red jungle chicken genomic DNA database, speculated that the source of New Yangzhou Chickens may originate from not only red jungle fowl, but also other jungle fowls. SCAR -markers confirmed that the two bands of OPAY02 type marker can be applied to genetic analyses because of its stability and reliability. Genotype equilibrium test showed that New Yangzhou chickens are in equilibrium on the two bands loci, two bands of OPAY02 type marker selection will be in favor of New Yangzhou chickens body weight breeding.

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Published

2005-08-15

Issue

Vol. 4 No. 9 (2005)

Section

Research Article

License

Copyright (c) 2005 Asian Network for Scientific Information

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

How to Cite

Dai, G., Wang, J., Olajide, O., Li, Q., Shen, H., Xie, K., Wang, Z., Wu, S., Gu, Y., & Zhang, G. (2005). Study on Molecular OPAY02-Scar Markers Related to Early Body Weight of New Yangzhou Chickens. International Journal of Poultry Science, 4(9), 683–688. https://doi.org/10.3923/ijps.2005.683.688

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