Detection of InvA Gene in Isolated Salmonella from Broilers by PCR Method


Authors

  • T. Zahraei Salehi Department of Microbiology and Immunology, Faculty of Veterinary Medicine, Tehran University, P.O. Box: 14155-6453, Tehran, Iran
  • M. Mahzounieh Department of Microbiology and Immunology, Faculty of Veterinary Medicine, Tehran University, P.O. Box: 14155-6453, Tehran, Iran
  • A. Saeedzadeh Department of Pathobiology, Faculty of Veterinary Medicine, Sharekord University, Iran

DOI:

https://doi.org/10.3923/ijps.2005.557.559

Keywords:

Broiler, invA gene, Iran, polymerase chain reaction (PCR), Salmonella

Abstract

The presence of Salmonella was detected in 192 samples of poultry carcasses from poultry farms in Shiraz province (Iran). A total of 30 Salmonella isolates were found in chicken samples (15.6%), by conventional culturing and confirmed by PCR and serology methods. Strains of serogroup D1 were the most prevalent strains, followed by serogroups C1, B and C2. All strains were subjected to Salmonella-specific gene (invA) and were confirmed as Salmonella positive by the predicted product a 284-bp DNA fragment.

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Published

2005-07-15

Issue

Vol. 4 No. 8 (2005)

Section

Research Article

License

Copyright (c) 2005 Asian Network for Scientific Information

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

How to Cite

Salehi, T. Z., Mahzounieh, M., & Saeedzadeh, A. (2005). Detection of InvA Gene in Isolated Salmonella from Broilers by PCR Method. International Journal of Poultry Science, 4(8), 557–559. https://doi.org/10.3923/ijps.2005.557.559