Impact of Microbial Enzymes on Growth Performance, Micronutrient Digestibility, Tissue Protein Contents and Endogenous Enzyme Activities of Broiler Chickens Fed on Vegetable Protein Diets
DOI:
https://doi.org/10.3923/ijps.2014.555.561Keywords:
Amino acid, broiler chickens, digestibility, enzyme, enzyme activities, mineral, tissue protein, vegetable-based dietsAbstract
A study was conducted to investigate the effects of dietary protein sources and microbial enzyme supplementation on amino acid and mineral digestibility, tissue protein contents and endogenous enzyme activities of broiler chickens. Ross 308 day-old male broiler chicks (n = 160) were assigned randomly to four dietary treatments, each replicated five times, eight chicks per replicate, in a 2 x 2 factorial design. Two basal diets were formulated with soybean (SBM) and canola (CM) meals at a ratio of 75:25, respectively, along with basal grains and fed to the birds as such or supplemented with enzymes from 1 to 35 days. Enzymes had a positive effect on the gross responses (feed intake, body weight and feed conversion ratio) of the broiler chickens at 35 days. However, feed intake was significantly different (p<0.001) between protein sources but this did not affect (p>0.05) body weight and feed conversion ratio (FCR) between 1 and 35 days. Tissue protein contents and endogenous enzyme activities at 21 days (except for maltase) were unaffected by dietary sources and microbial enzyme supplementation. The activity of maltase was higher (p<0.05) on CM diets than on SBM diets, but this effect was absent when diets were supplemented with enzymes. The digestibility of threonine and lysine, measured at 35 days, was significantly (p<0.01) higher on SBM diets than on CM diets. Similarly the digestibility of valine, isoleucine and leucine was also higher (p<0.05) on SBM diet than in CM diet. Addition of enzymes also significantly (p<0.05) increased the digestibility of histidine, threonine, lysine and isoleucine, but not the digestibility of other amino acids measured at this period (35d). Enzymes had no effect (p>0.05) on mineral digestibility of broilers over the test period. The digestibility of Cu, Zn and Mg was increased (p<0.05) on CM diet, whereas Ca digestibility was higher on SBM diet at 35 days. It can be concluded that the improved growth of the birds might be a result of increased digestibility of amino acids and increase in feed consumption.
References
Anderson, D.L. and L.J. Henderson, 1986. Sealed chamber digestion for plant nutrient analyses. Agron. J., 78: 937-939.
Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-254.
Bhuiyan, M.M., A.F. Islam and P.A. Iji, 2010. Response of broiler chickens to diets containing artificially dried high-moisture maize supplemented with microbial enzymes. South Afr. J. Anim. Sci., 40: 348-362.
Bryden, W.L., X. Li, G. Ravindran, L.I. Hew and V. Ravindran, 2009. Ileal digestible amino acid values in feedstuffs for poultry. RIRDC Publication No. 09/071, June 2009.
Cowieson, A.J. and V. Ravindran, 2008. Effect of exogenous enzymes in maize-based diets varying in nutrient density for young broilers: Growth performance and digestibility of energy, minerals and amino acids. Br. Poult. Sci., 49: 37-44.
CEC., 2000. Council Decision of 4 December 2000 concerning certain protection measures with regard to transmissible spongiform encephalopathies and the feeding of animal protein. Off. J. Eur. Communit., L306: 32-33.
Duke, G.E., 1986. Alimentary Canal: Secretion and Digestion, Special Digestive Functions, and Absorption. In: Avian Physiology, Sturkie, P.D. (Ed.). Springer-Verlag, New York, ISBN: 978-1-4612-9335-4, pp: 289-302.
FAO., 2004. Protein Sources for the Animal Feed Industry: Expert Consultation and Workshop Bangkok, 29 April-3 May 2002. Food and Agriculture Organization of the United Nations, Rome, Italy, Pages: 349.
Gatel, F., 1994. Protein quality of legume seeds for nonruminant animals: A literature review. Anim. Feed Sci. Technol., 45: 317-348.
Im, H.L., V. Ravindran, G. Ravindran, P.H. Pittolo and W.L. Bryden, 1999. The apparent metabolisable energy and amino acid digestibility of wheat, triticale and wheat middlings for broiler chickens as affected by exogenous xylanase supplementation. J. Sci. Food Agric., 79: 1727-1732.
Hossain, M.A., A.F. Islam and P.A. Iji, 2012. Energy utilization and performance of broiler chickens raised on diets with vegetable proteins or conventional feeds. Asian J. Poult. Sci., 6: 117-128.
Iji, P.A., A. Saki and D.R. Tivey, 2001. Body and intestinal growth of broiler chicks on a commercial starter diet. 2. Development and characteristics of intestinal enzymes. Br. Poult. Sci., 42: 514-522.
Jiang, Z., Y. Zhou, F. Lu, Z. Han and T. Wang, 2008. Effects of different levels of supplementary alpha-amylase on digestive enzyme activities and pancreatic amylase mRNA expression of young broilers. Asian-Aust. J. Anim. Sci., 21: 97-102.
Le Goff, G. and J. Noblet, 2001. Comparative digestibility of dietary energy and nutrients in growing pigs and adult sows. J. Anim. Sci., 79: 2418-2427.
Lazaro, R., M.A. Latorre, P. Medel, M. Gracia and G.G. Mateos, 2004. Feeding regimen and enzyme supplementation to rye-based diets for broilers. Poult. Sci., 83: 152-160.
McPherson, G.A., 1985. Lowry Program Software. Elsevier Biosoft, Cambridge, UK.
Minitab, 2000. Minitab Statistical Software User's Guide 2: Data Analysis and Quality Tools. Minitab Inc., State College, PA., USA.
Newkirk, R.W. and H.L. Classen, 2002. The effects of toasting canola meal on body weight, feed conversion efficiency and mortality in broiler chickens. Poult. Sci., 81: 815-825.
Nitsan, Z., Y. Dror, I. Nir and N. Shapira, 1974. The effects of force-feeding on enzymes of the liver, kidney, pancreas and digestive tract of chicks. Br. J. Nutr., 32: 241-247.
Naseem, M.Z., S.H. Khan and M. Yousuf, 2006. Effect of different level of canola meal on broiler production performance during two phases of growth. Pak. Vet. J., 26: 129-134.
Omenka, R.O. and G.N. Anyasor, 2010. Vegetable-based feed formulation on poultry meat quality. Afr. J. Food Agric. Nutr. Dev., 10: 2001-2011.
Peng, Y.L., Y.M. Guo and J.M. Yuan, 2003. Effects of microbial phytase replacing partial inorganic phosphorus supplementation and xylanase on the growth performance and nutrient digestibility in broilers fed wheat-based diets. Asian-Austr. J. Anim. Sci., 16: 239-247.
Pirgozliev, V., M.R. Bedford, T. Acamovic, P. Mares and M. Allymehr, 2011. The effects of supplementary bacterial phytase on dietary energy and total tract amino acid digestibility when fed to young chickens. Br. Poult. Sci., 52: 245-254.
Ravindran, V., S. Cabahug, G. Ravindra, P.H. Selle and W.L. Bryden, 2000. Response of broiler chickens to microbial phytase supplementation as influenced by dietary phytic acid and non-phytate phosphorous levels. II. Effects on apparent metabolisable energy, nutrient digestibility and nutrient retention. Br. Poult. Sci., 41: 193-200.
Ravindran, V., P.H. Selle, G. Ravindran, P.C.H. Morel, A.K. Kies and W.L. Bryden, 2001. Microbial phytase improves performance, apparent metabolizable energy and ileal amino acid digestibility of broilers fed a lysine-deficient diet. Poult. Sci., 80: 338-344.
Singh, K.S. and B. Panda, 1992. Poultry Nutrition. Kalyani Publisher, New Delhi, India, pp: 57-61.
Short, F.J., P. Gorten, J. Wiseman and K.N. Boorman, 1996. Determination of titanium dioxide added as an inert marker in chicken digestibility studies. Anim. Feed Sci. Technol., 59: 215-221.
Smits, C.H.M. and G. Annison, 1996. Non-starch plant polysaccharides in broiler nutrition-towards a physiologically valid approach to their determination. World's Poult. Sci. J., 52: 203-221.
Sarwar, M., M.A. Khan and Z. Iqbal, 2002. Feed resources for livestock in Pakistan. Int. J. Agric. Biol., 4: 186-192.
Shirazi-Beechey, S.P., M.W. Smith, Y. Wang and P.S. James, 1991. Postnatal development of lamb intestinal digestive enzymes is not regulated by diet. J. Physiol., 437: 691-698.
Sebastian, S., S.P. Touchburn, E.R. Chavez and P.C. Lague, 1997. Apparent digestibility of protein and amino acids in broiler chickens fed a corn-soybean diet supplementation with microbial phytase. Poult. Sci., 76: 1760-1769.
Selle, P.H., V. Ravindran, R.A. Caldwell and W.L. Bryden, 2000. Phytate and phytase: Consequences for protein utilisation. Nutr. Res. Rev., 13: 255-278.
Thompson, L.U. and H. Yoon, 1984. Starch digestibility as affected by polyphenols and phytic acid. J. Food Sci., 49: 1228-1229.
Wapnir, R.A., 1989. Protein Digestion and the Absorption of Mineral Elements. In: Mineral Absorption in the Monogastric GI Tract, Dintzis, F.R. and J.A. Laszlo (Eds.). Springer-Verlag, USA., ISBN: 978-1-4684-9113-5, pp: 95-115.
Zanella, I., N.K. Sakomura, F.G. Silversides, A. Fiqueirdo and M. Pack, 1999. Effect of enzyme supplementation of broiler diets based on corn and soybeans. Poult. Sci., 78: 561-568.
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