Organic Acid Formulation and Dip to Control Listeria monocytogenes in Hot Dogs
DOI:
https://doi.org/10.3923/ijps.2012.469.473Keywords:
Hot dogs, lactate, Listeria monocytogenes, organic acids, sodium diacetateAbstract
Commercially, hotdogs are handled after the cooking potentially causing contamination. Since consumers may eat hotdogs without reheating, the presence of Listeria Monocytogenes (LM) is a concern. In this study, treatments with organic acids in the raw product and as a post-cook dip were evaluated for their ability to suppress the growth of LM. Beef hotdogs were formulated with organic acids, cooked, cooled, inoculated with LM and then dipped in organic acid treatments. Treatments included: 1) Potassium Lactate (PL) in the formulation and Sodium Lactate (SL) in the dip, 2) PL in the formulation and SL with Sodium Diacetate (SD) in the dip, 3) SL and SD in the formulation and SL in the dip and 4) SL and SD in the formulation and SL and SD in the dip. Positive (inoculated) and negative (non-inoculated) controls were formulated with no organic acids and dipped in distilled water. All hotdogs were stored at 4°C and the number of LM was evaluated on day 0, 7, 14, 21, 28, 42 and 56. When compared to the positive control, all treatments resulted in significantly decreased LM numbers over time. The SL/SD with SL dip and SL/SD with SL/SD dip were the most effective treatments.
References
Benjamin, A., 1998. How Buffers Work: Part 2. In: Understanding Acid-Base, Kelly, P. (Ed.). Williams and Wilkins, Baltimore, Maryland, pp: 27-38.
Berg, J.M., J.L. Tymoczko and L. Stryer, 2002. Appendix B: Acidity Constants. 5th Edn., W.H. Freeman and Company, New York.
Blom, H., E. Nerbrink, R. Dainty, T.T. Hagtved, E. Borch, H. Nissen and T. Nesbakken, 1997. Addition of 2.5% lactate and 0.25% acetate controls growth of Listeria monocytogenes in vacuum-packed, sensory-acceptable servelat sausage and cooked ham stored at 4°C. Int. J. Food Microbiol., 38: 71-76.
De Vegt, B., 1999. Listeria and Salmonella control. Enlargement Europe, Purac, pp: 1-4.
Doyle, M.E., 1999. Use of organic acids to control Listeria in meat. Food Research Institute, November 1999, pp: 2-4. http://fri.wisc.edu/docs/pdf/listeria.pdf.
Johnson, J.L., M.P. Doyle and R.G. Cassens, 1990. Listeria monocytogenes and other sp. in meat and meat products: A review. J. Food Prot., 53: 81-91.
Juncher, D., C.S. Vestergaard, J. Soltoft-Jensen, C.J. Wever, G. Bertelsen and L.H. Skibsted, 2000. Effects of chemical hurdles on microbiological and oxidative stability of a cooked cured emulsion type meat product. Meat Sci., 55: 483-491.
Le Marc, Y., V. Huchet, C.M. Bourgeois, J.P. Guyonnet, P. Mafart and D. Thuault, 2002. Modeling the growth kinetics of Listeria as a function of temperature, pH and organic acid concentration. Int. J. Food Microbiol., 73: 219-237.
Ngutter, C. and C. Donnelly, 2003. Nitrite-induced injury of Listeria monocytogenes and the effect of selective versus nonselective recovery procedures on its isolation from frankfurters. J. Food Prot., 66: 2252-2257.
Phan-Thanh, L., F. Mahouin and S. Alige, 2000. Acid responses of Listeria monocytogenes. Int. J. Food Microbiol., 55: 121-126.
Samelis, J., G.K. Bedie, J.N. Sofos, K.E. Belk, J.A. Scanga and G.C. Smith, 2002. Control of Listeria monocytogenes with combined antimicrobials after postprocess contamination and extended storage of frankfurters at 4°C in vacuum packages. J. Food Prot., 65: 299-307.
SAS Institute., 1993. SAS® User's Guide: Statistics. SAS Institute Inc, Carry, NC.
Shahamat, M., A. Seaman and M. Woodbine, 1980. Influence of Sodium Chloride, pH and Temperature on the Inhibitory Activity of Sodium Nitrite on Listeria monocytogenes. In: Microbial Growth and Survival in Extremes of Environment, Microbial Growth and Survival in Extremes of Environment, Academic Press, London, pp: 227-237.
Stekelenburg, F.K., 2003. Enhanced inhibition of Listeria monocytogenes in Frankfurter sausage by the addition of potassium lactate and sodium diacetate mixtures. Food Microbiol., 20: 133-137.
Vasseur, C., L. Baverel, M. Hebraud and J. Labadie, 1999. Effect of osmotic, alkaline, acid or thermal stresses on the growth and inhibition of Listeria monocytogenes. J. Applied Microbiol., 86: 469-476.
Vogel, B.F., Y.Y. Ng, G. Hyldig, M. Mohr and L. Gram, 2006. Potassium lactate combined with sodium diacetate can inhibit growth of Listeria monocytogenes in vacuum-packed cold-smoked salmon and has no adverse sensory effects. J. Food. Prot., 69: 2134-2142.
Zhu, M., M. Du, J. Cordray and D.U. Ahn, 2005. Control of Listeria monocytogenes Contamination in ready-to-eat meat products. Comp. Rev. Food Sci. Food Saf., 4: 34-42.
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