The Effect of Graded Levels of Dietary Methionine on the Haematology and Serum Biochemistry of Broilers
DOI:
https://doi.org/10.3923/ijps.2010.158.161Keywords:
Broilers, dietary methionine, haematology, serum biochemistryAbstract
Graded levels of dietary methionine on the haematology and serum biochemistry were investigated in broilers. One hundred and fifty broiler chicks (Arbor acre breed) were use, the chicks were divided into 5 treatments consisting of 6 replicates of five chicks each. The chicks were kept in floor pens. The study lasted for 56 days at the teaching and research farm of the university of Ibadan Nigeria. No significant differences (p>0.05) were observed in the PCV, RBC and WBC values of broilers fed the different levels of methionine inclusion, at the finisher phase, but at starter phase significant differences (p<0.05) were observed, with the WBC values increasing as the inclusion rate of methionine increased. Total protein value of 4.80g/100ml and 4.48 g/100 ml were obtained for treatments 4 and 5 respectively which were not significantly (p>0.05) from each other. There were wide variations in the glucose concentration of the birds, the highest glucose concentration was observed with birds on diet 2 (220.90) while the least was observed for birds fed the control diet though significant differences (p<0.05) were observed it did not follow a particular order.
References
AOAC., 1990. Official Method of Analysis. 15th Edn., Association of Official Analytical Chemists, Washington, DC., USA., pp: 66-88.
Beutler, E. and W.J. Williams, 2001. Williams Hematology. 6th Edn., McGraw-Hill Medical Publishing Division, New York.
Brody, T., 1994. Nutritional Biochemistry. Academic Press Inc., San Diego, CA.
Cooper, G.R. and V. McDaniel, 1970. Standard methods of clinical. Chemistry, 6: 159-159.
Fudge, A.M., 2000. Laboratory Medicine-avian and Exotic Pets. W.B. Saunders Co., Philadelphia, pp: 486.
Herbert, R., J. Nanney, J.S. Spano, W.M. Pedersoli and L.M. Krista, 1989. Erythrocyte distribution in ducks. Am. J. Vet. Res., 50: 958-960.
Huyghebaert, G., M. Pack and G.D. Groote, 1994. Influence of protein concentration on the response of broilers to supplemental DL-methionine. Arch. Geflugelk, 59: 23-29.
Kelly, W.R., 1979. Veterinary Clinical Diagnosis. 2nd Edn., Bailliere Tindall, London, pp: 261-300.
Latimer, K.S. and D. Bienzle, 2000. Determination and Interpretation of the Avian Leukogram. In: Schalms Veterinary Hematology, Feldman, B.F., J.G. Zinkl and N.C. Jain (Eds.). 5th Edn., Lippincott, Williams and Wilkisn, Philadelphia, pp: 417-432.
Lindsay, D.B., 1977. The Effect of Feeding Patterns and Sampling on Blood Parameters. In: British Society of Animal Production, Lister, D. (Ed.). Occasional Public., Edinburgh, UK., pp: 99-120.
Maxwell, M.H., 1993. Avian blood leucocyte responses to stress. World's Poult. Sci. J., 49: 34-43.
NRC, 1994. Nutrients Requirements of Poultry. 9th Edn., National Academic Press, Washington, DC., pp. 40-41.
Olomu, J.M. and S.A. Offiong, 1980. Effect of protein and energy The effects of different protein and energy levels and time of change from starter to finisher ration on the performance of broiler chickens in the tropics. Br. Poult. Sci., 59: 828-835.
Oluyemi, J.A. and R.A. Roberts, 1979. Poultry Production in Warm Wet Climate. Mcmillian International College, London.
Pestal, G.M.N., A.C. Banevenga, Haroer and M.L. Sunde, 1981. Factor influencing the assessment of the availability of chorine in feedstuffs. Poult. Sci., 60: 188-196.
Peters, T., G.T. Biamonte and B.T. Doumas, 1982. Protein (Total Protein) in Serum Urine and Cerebrospinal Fluid Albumin in Chemistry. Vol. 9, American Association for Clinical Chemistry, Washington, DC.
Ritchie, R.F. and O.E. Navolotskaia, 1996. Serum Proteins in Clinical Medicine. Foundation for Blood Research, Scarborough, ME.
Roschlan, P., E. Bernet and W. Gruber, 1974. Ezymatische best immungoles gesamt Cholesterius in serum. J. Clin. Chem. Biochem., 12: 403-407.
Schuttle, J.B. and J. De Jong, 1996. Biological efficiency of DL-methionine hydroxyl analog: Free acid compared to DI methionine in broiler chick as determined by performance and breast meat yield. Agribiol. Res., 49: 74-82.
Thrall, M.A., 2004. Veterinary Hematology and Clinical Chemistry. Lippincott Williams and Wilkins, Philadelphia, Pages: 518.
Downloads
Published
Issue
Section
License
Copyright (c) 2010 Asian Network for Scientific Information
This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
