Effect of Antigen Source on the Sensitivity of Avian Influenza Virus Haemagglutination Inhibition Test


Authors

  • Asma Jabeen National Reference Laboratory for Poultry Diseases, Animal Sciences Institute, National Agricultural Research Centre, Park Road, Islamabad, Pakistan
  • Khalid Naeem National Reference Laboratory for Poultry Diseases, Animal Sciences Institute, National Agricultural Research Centre, Park Road, Islamabad, Pakistan
  • Zaheer Ahmed National Reference Laboratory for Poultry Diseases, Animal Sciences Institute, National Agricultural Research Centre, Park Road, Islamabad, Pakistan
  • Naila Siddique National Reference Laboratory for Poultry Diseases, Animal Sciences Institute, National Agricultural Research Centre, Park Road, Islamabad, Pakistan

DOI:

https://doi.org/10.3923/ijps.2010.28.31

Keywords:

Antibody detection, avian influenza virus, haemagglutination inhibition

Abstract

The effect of variation in the source of Avian Influenza Virus (AIV) antigen on the sensitivity of Haemagglutination Inhibition (HI) test was examined. For this purpose, HI antigen of AIV subtypes H5 and H7 were either prepared or procured from different sources and used to determine the antibody levels against AIV subtypes H5 and H7 in chickens, using HI test. The HI test was standardized using 30 serum samples collected from a flock experimentally vaccinated against AIV subtypes H5N9 and H7N1 under controlled conditions. The test serum samples were collected from the field among chickens vaccinated against AIV subtypes H5N9 and H7N1 vaccines and subjected to HI test using a variety of HI antigens belonging to H5 and H7 subtypes. The results reflected that the type of AIV strain used in the HI antigens affected the levels of antibody titre determination from the same samples. The effect of this variation on reliability of data regarding sero-surveillance and vaccine efficacy testing for avian influenza among poultry is discussed.

References

Fouchier, R.A.M., V. Munster, A. Wallensten, T.M. Bestebroer and S. Herfst et al., 2005. Characterization of a novel influenza A virus hemagglutinin subtype (H16) obtained from black-headed gulls. J. Virol., 79: 2814-2822.

Hierholzer, J.C. and M.T. Suggs, 1969. Standardized viral haemagglutination and haemagglutination-inhibition tests. Applied Microbiol., 18: 824-833.

Lu, B., R.G. Webster and V.S. Hinshaw, 1982. Failure to detect haemagglutination-inhibiting antibodies with intact avian influenza virions. Infect. Immun., 38: 530-535.

Mo, I.P., M. Brugh, O.J. Fletcher and D.E. Swayne, 1997. Comparative pathology of chickens experimentally inoculated with avian influenza viruses of low and high pathogenicity. Avian Dis., 41: 125-136.

Matthews, R.E.F., 1979. Classification and nomenclature of viruses. Third Report of International Committee on Taxonomy of Viruses.

Olsen, C.W., A. Karasin and G. Erickson, 2003. Characterization of swine like Reassortant H1N2 influenza virus isolated from wild duck in United States. Virus Res., 93: 115-121.

Suarez, D.L. and S. Schultz-Cherry, 2000. Immunology of avian influenza Virus: A Review. Dev. Comp. Immunol., 24: 269-283.

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Published

2009-12-15

Issue

Vol. 9 No. 1 (2010)

Section

Research Article

License

Copyright (c) 2010 Asian Network for Scientific Information

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

How to Cite

Jabeen, A., Naeem, K., Ahmed, Z., & Siddique, N. (2009). Effect of Antigen Source on the Sensitivity of Avian Influenza Virus Haemagglutination Inhibition Test. International Journal of Poultry Science, 9(1), 28–31. https://doi.org/10.3923/ijps.2010.28.31