Phylogenetic Analysis of the Nucleoprotein Gene of Newcastle Disease Vaccine Viruses in India
DOI:
https://doi.org/10.3923/ijps.2009.1137.1150Keywords:
Avian paramyxovirus 1, genome, immunodominant epitope, newcastle disease, nucleoprotein geneAbstract
The Nucleoprotein (NP) gene of four different vaccine viruses of NDV commonly used in India namely D58, LaSota, F and K were characterized to find out variations if any. The NP gene was observed to have 1747 nucleotides with a coding sequence of 1470 nucleotides coding for 489 amino acids in all four vaccine viruses irrespective of their virulence to chickens. The variations at the coding sequence were restricted mostly to positions 401-489 and positions between 1 and 400, irrespective of pathogenicity to chickens remain conserved. The epitopes in nucleoprotein gene was also predicted. Among the 20 different epitopes predicted in the NP gene, one epitope with sequence 447FLDLMRA453 was found conserved in all vaccine viruses and was also found to be immunodominant.
References
Ananth, R., J. John-Kirubaharan, M.L.M. Priyadarshini and A. Albert, 2008. Isolation of newcastle disease viruses of high virulence in unvaccinated healthy village chickens in South India. Int. J. Poult. Sci., 7: 368-373.
Aier, K., 1930. A study on Madras fowl pest. Indian Vet. J., 8: 346-352.
Sulochana, S., R.M. Pillai, G.K. Nair, D. Sudharma and P.K. Abdulla, 1981. Epizootology of Newcastle disease in Indian hons crows. Vet. Rec., 109: 249-249.
Gurkipal, S.A., P.S. Mangal and B.S. Gill, 1989. Isolation of Newcastle disease virus (avian paramyxovirus-I) from pigeons (Colubia livia) in India. Curr. Sci., 58: 154-155.
Errington, W., M. Steward and P.T. Emmerson, 1995. A diagnostic immunoassay for Newcastle disease virus based on the nucleocapsid protein expressed by a recombinant baculovirus. J. Virol. Methods, 55: 357-365.
Makkay, A.M., P.J. Krell and E. Nagy, 1999. Antibody detection based differential ELISA for NDV-infected or vaccinated chickens versus NDV HN subunit vaccinated chickens. Vet. Microbiol., 66: 209-222.
Mebatsion, T., M.J.M. Koolen, L.T.C. de Vaan, N. de Haas and M. Braber et al., 2002. Newcastle Disease Virus (NDV) marker vaccine: An immunodominant epitope on the nucleoprotein gene of NDV can be deleted or replaced by a foreign epitope. J. Virol., 76: 10138-10146.
Winterfield, R.W., C.L. Goldman and E.H. Seadale, 1957. Newcastle disease immunization studies vaccination of chickens with B1, F and LaSota strains of Newcastle disease virus administered through the drinking water. Poult. Sci., 36: 1076-1088.
Asplin, F.D., 1952. Immunization against ND with a virus of low virulence and observations on substantial infection in partially resistant fowls. Vet. Rec., 64: 245-245.
Komarov, A. and L. Goldsmith, 1946. Preliminary observation on the modification of a strain of Newcastle disease virus by intracerebral passage through ducklings. Br. Vet. J., 102: 212-212.
European Communities, 2004. Consolidated text produced by the CONSLEG system of the Office for Official Publications of the European Communities. http://ec.europa.eu/food/food/animalnutrition/labelling/consleg_29_en.pdf.
Harper, D.R., 1989. A novel plaque assay systems for paramyxoviruses. J. Virol. Methods, 25: 347-350.
Freshney, R., 2000. Culture of Animal Cells: A Manual of Basic Technique. 5th Edn., Wiley-Liss, New York, NY, USA., pp: 414-415.
Kumanan, K., 1989. Studies on immunogenic proteins of Newcastle disease virus. Ph.D. Thesis, Tamil Nadu Veterinary and Animal Sciences University, Chennai.
Reed, L.V. and H. Muench, 1938. A single method of evaluating 50% end points. Am. J., 27: 493-493.
Krishnamurthy, S. and S.K. Samal, 1998. Neucleotide sequences of the trailer, nucleocapside protein gene and intergenic regions of Newcastle disease virus strain Beaudette c and compltions of the entire genome sequence. J. Gen. Virol., 19: 2419-2424.
Rose, R.N., D.J. Folds, H. Chifford and M.R. Nakamura, 1997. Manual of Clinical Laboratory Immunology. 5th Edn., ASM Press, USA., pp: 24.
Ishida, N., H. Taira, T. Omata, K. Mizimoto, S. Hattori, K. Iwasaki and M. Kawakita, 1986. Sequence of 2,617 nucleotides from 3' end of Newcastle disease virus genome RNA and the predicted amino acid sequence of viral NP protein. Nucleic Acids Res., 14: 6551-6554.
De Leeuw, O. and B. Peeters, 1999. Complete nucleotide sequence of Newcastle disease virus: Evidence for the existence of a new genus within the subfamily Paramyxovirinae. J. Gen. Virol., 80: 131-136.
Ward, M.D.W., F.J. Fuller, Y. Mehrotra and E.V. De Buysscher, 2000. Nucleotide sequence and vaccinia expression of the nucleoprotein of a highly virulent, neurotropic strain of Newcastle disease virus. Avian Dis., 44: 34-44.
Huang, Z., S. Krishnamurthy, A. Panda and S.K. Samal, 2003. Newcastle disease virus V protein is associated with viral pathogenesis and function as an alpha interferon antagonist. J. Virol., 77: 8676-8685.
Kho, C.L., W.S. Tan, B.T. Tey and K. Yusoff, 2003. Newcastle disease virus nucleocapsid protein: Self assembly and length determination domains. J. Gen. Virol., 84: 2163-2168.
Rosenblatt, S., O. Eizenberg, R. Ben-Levy, V. Larie and W.J. Bellani, 1985. Sequence homology within morbilli viruses. J. Virol., 53: 684-690.
Sanchez, A., A.K. Banerjee, Y. Furuichi and M.K. Richerdson, 1986. Conserved structures among the nucleocapsid proteins of the paramyxoviridae: Complete nucleotide sequence of human parainfluenza virus type 3 NP RNA. Virology, 152: 171-180.
Millar, N.S., P. Chambers and P.T. Emerson, 1986. Nucleotide sequence analysis of the haemagglutinin-neuraminidase gene of newcastle disease virus. J. Gen. Virol., 67: 1917-1927.
Yusoff, K., N.S. Millar, P. Chambers and P.T. Emmerson, 1987. Nucleotide sequence analysis of the L gene of Newcastle disease virus: Homologies with sendai and vesicular viruses. Nucl. Acids. Res., 15: 3961-3976.
Phillips, R.J., A.C.R. Sason and P.T. Emmerson, 1998. Nucleotide sequence of the 5 terminus of Newcastle disease virus and assembly of the complete genomic sequence agreement with the rule of six. Arch. Virol., 143: 1993-2002.
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