Assessment of Pathogenic Potential of Avian Influenza Viruses by MDCK Cell Culture


Authors

  • B.P. Shankar High Security Animal Disease Laboratory, IVRI, Bhopal, India
  • R. N. Sreenivas Gowda Veterinary College, Hebbal, Bangalore-24, Karnataka, India
  • B. Pattnaik High Security Animal Disease Laboratory, IVRI, Bhopal, India
  • B. H. Manjunatha Prabhu Molecular Biology Genetics Units, JNCASR, Jakkur, Bangalore, India
  • R.P. Kamal High Security Animal Disease Laboratory, IVRI, Bhopal, India
  • B.K. Sreenivas IAH&VB, Hebbal, Bangalore, India
  • H.S. Madhusudhan Veterinary College, Hebbal, Bangalore-24, Karnataka, India
  • D. Ranjith Veterinary College, Hebbal, Bangalore-24, Karnataka, India
  • H.K. Pradhan High Security Animal Disease Laboratory, IVRI, Bhopal, India

DOI:

https://doi.org/10.3923/ijps.2009.462.464

Keywords:

Avian influenza H5N1, CPE, India, MDCK

Abstract

The influenza viruses are sub-classified in to two pathotypes of Highly Pathogenic Avian Influenza (HPAI) and Low Pathogenic Avian Influenza (LPAI) viruses on the basis of the pathogenicity of AIV in domestic poultry. Samples of H5N1 (7966/06 and 7972/06) and the H9N1 (5844/05) viruses were also grown on MDCK cell. All these produced cytopathic effect within 72 h. Normally, the nonpathogenic AIV does not produce CPE in MDCK cells. However, CPE can be produced if trypsin is incorporated while, culturing the viruses. In the present study, without the addition of trypsin the viruses produced CPE. On FAT both cytoplasmic and nuclear florescence was observed. It is also known that the viruses which produce CPE in absence of trypsin are pathogenic. It proved beyond that the H5N1 and H9N1 viruses isolated in the present study were pathogenic based on cell culture study.

References

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Murphy, B.R. and R.G. Webster, 1996. Orthomyxoviruses. In: Field Virology, Fields, B.N., D.M. Knipe and P.M. Howley (Eds.). 3rd Edn., Lippincott, Raven, PA., pp: 1397-1445.

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Published

2009-04-15

Issue

Vol. 8 No. 5 (2009)

Section

Research Article

License

Copyright (c) 2009 Asian Network for Scientific Information

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

How to Cite

Shankar, B., Gowda, R. N. S., Pattnaik, B., Prabhu, B. H. M., Kamal, R., Sreenivas, B., Madhusudhan, H., Ranjith, D., & Pradhan, H. (2009). Assessment of Pathogenic Potential of Avian Influenza Viruses by MDCK Cell Culture. International Journal of Poultry Science, 8(5), 462–464. https://doi.org/10.3923/ijps.2009.462.464

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