Effects of Propolis as an Organic Growth Promoter on Growth Performance and Carcass Characteristics of Cobb 500 Broiler Chickens


Authors

  • Glory Zerah Azza ORCiD Department of Animal, Aquaculture and Range Sciences (DAARS), College of Agriculture, Sokoine University of Agriculture, Morogoro, Tanzania
  • Charles Moses Lyimo ORCiD Department of Animal, Aquaculture and Range Sciences (DAARS), College of Agriculture, Sokoine University of Agriculture, Morogoro, Tanzania
  • Claudius David Luziga ORCiD Department of Veterinary Anatomy, College of Veterinary Medicine and Biomedical Sciences (CVMBS), Sokoine University of Agriculture, Morogoro, Tanzania
  • Robinson Hammerthon Mdegela ORCiD Department of Veterinary Medicine and Public Health, College of Veterinary Medicine and Biomedical Sciences (CVMBS), Sokoine University of Agriculture, Morogoro, Tanzania
  • Sebastian Wilson Chenyambuga ORCiD Department of Animal, Aquaculture and Range Sciences (DAARS), College of Agriculture, Sokoine University of Agriculture, Morogoro, Tanzania

DOI:

https://doi.org/10.66920/ijps.2026.78.86

Keywords:

Broiler chickens, carcass characteristics, growth performance, meat quality, organic growth promoter, propolis extract

Abstract

Objective: This study aimed to evaluate the efficacy of propolis extract as a natural organic growth promoter on growth performance, carcass characteristics and meat quality of Cobb 500 broiler chickens, in comparison with a commercial broiler booster.

Materials and Methods: A total of 100 day-old broiler chicks were randomly allocated to five treatment groups: Control (CTR), commercial broiler booster (CBB) and propolis extract administered via drinking water at concentrations of 100 g/L (P1), 200 g/L (P2) and 300 g/L (P3). Birds were reared under deep-litter conditions for six weeks and fed standard starter, grower and finisher diets. Growth performance, carcass traits, organ weights and meat quality parameters were assessed at the end of the experimental period.

Results: Propolis supplementation at 100 g/L (P1) significantly improved feed conversion ratio, weight gain and muscle development, particularly breast and thigh weights, compared with the control group. Despite lower feed intake, birds in P1 exhibited higher weight gain, indicating superior feed efficiency. Meat quality analysis showed that P1 resulted in the lowest shear force and pH values, along with higher redness (a*) values, suggesting improved meat tenderness and enhanced antioxidant status. Organ weights, including liver, intestine and gizzard, were significantly higher in P1, indicating improved metabolic and digestive activity. In contrast, higher propolis concentrations (P2 and P3) were associated with reduced performance, demonstrating a dose-dependent response. Carcass weights were numerically higher in P1 and P3, although these differences were not statistically significant.

Conclusion: Propolis extract administered at 100 g/L in drinking water effectively enhanced growth performance, carcass characteristics and meat quality in broiler chickens. This dosage represents an optimal, natural and sustainable alternative to synthetic growth promoters, supporting improved poultry productivity while reducing reliance on antibiotics.

References

Ayalew, H., H. Zhang, J. Wang, S. Wu, K. Qiu and D. Chanie et al, 2022. Potential feed additives as antibiotic alternatives in broiler production. Front. In Vet. Sci., Vol. 9. 10.3389/fvets.2022.916473

United Republic of Tanzania, 2023. Speech by the Minister for Finance and Planning, Hon. Dr. Mwigulu Lameck Nchemba Madelu (MP.) presenting to the National Assembly, the estimates of government revenue and expenditure for 2023/24. Ministry of Finance and Planning, Dodoma, Tanzania. https://repository.mof.go.tz/items/186803e4-c24d-4369-b971-7ebf426ea89c

Mohamed, R.I., G.M. Mosaad and H.Y.A. El-wahab, 2018. Effect of feeding propolis on growth performance of broilers. J. Adv. Vet. Res., 8: 66-72.

Chuttong, B., K. Lim, P. Praphawilai, K. Danmek, J. Maitip and S. Hongsibsong et al, 2023. Exploring the functional properties of propolis, geopropolis, and cerumen, with a special emphasis on their antimicrobial effects. Foods, Vol. 12. 10.3390/foods12213909

Coneglian, J.L.B., S.L. Vieira, J. Berres and D.M.D. Freitas, 2010. Responses of fast and slow growth broilers fed all vegetable diets with variable ideal protein profiles. Rev. Bras. Zootecnia, Vol. 39. 10.1590/s1516-35982010000200015

Dalloul, C.C.R, 2015. Immunomodulatory role of probiotics in poultry and potential in ovo application. Benefic. Microbes, 6: 45-52.

Costa, M.C., J.A. Bessegatto, A.A. Alfieri, J.S. Weese, J.A.B. Filho and A. Oba, 2017. Different antibiotic growth promoters induce specific changes in the cecal microbiota membership of broiler chicken. PLoS ONE., Vol. 12. 10.1371/journal.pone.0171642

Abdulla, T.A. and M.G. Hassan, 2011. The effect of propolis feed supplementation on hygiene and performance of broiler chickens. Iraqi J. Vet. Sci., 25: 77-82.

Gaskins, H.R., C.T. Collier and D.B. Anderson, 2002. Antibiotics as growth promotants: Mode of action. Anim. Biotechnol., 13: 29-42.

Marangoni, F., G. Corsello, C. Cricelli, N. Ferrara, A. Ghiselli and A. Poli et al, 2015. Role of poultry meat in a balanced diet aimed at maintaining health and wellbeing: An Italian consensus document. Food Nutr. Res., Vol. 59. 10.3402/fnr.v59.27606

Khaligh, F., G. Sadeghi, A. Karimi and A. Vaziry, 2011. Evaluation of different medicinal plants blends in diets for broiler chickens. J. Med. Plants Res., 5: 1971-1977.

Denli, M., S. Cankaya, S. Silici, F. Okan and A.N. Uluocak, 2005. Effect of dietary addition of Turkish propolis on the growth performance, carcass characteristics and serum variables of quail (Coturnix coturnix Japonica). Asian-Australas. J. Anim. Sci., 18: 848-854.

O’Neill, J, 2016. Tackling drug-resistant infections globally: Final report and recommendations. Review on Antimicrobial Resistance. https://amr-review.org/sites/default/files/160518_Final%20paper_with%20cover.pdf

Konanç, K. and E. Öztürk, 2025. Propolis supplementation in broiler breeder chickens: Enhancing meat quality. Black Sea J. Agric., 8: 412-420.

Council, N.R, 1994. Nutrient Requirements of Poultry. 9th ed., National Academies Press, Washington, DC, USA, ISBN: 978‑0‑309‑04892‑7. Pages: 176.

Toghyani, M., M. Toghyani and S.A. Tabeidian, 2011. Effect of probiotic and prebiotic as antibiotic growth promoter substitutions on productive and carcass traits of broiler chicks. Proceeding of 2011 international conference on food engineering and biotechnology, ACSIT Press, Singapore, pp: 82-86.

Klarić, I., M. Domaćinović, V. Šerić, I. Miškulin, M. Pavić and K. Paradinović, 2018. Effects of bee pollen and propolis on performance, mortality, and some haematological blood parameters in broiler chickens. Slovenian Vet. Res., Vol. 55. 10.26873/svr-385-2018

Seddon, M., Y.H. Looi and A.M. Shah, 2006. Oxidative stress and redox signalling in cardiac hypertrophy and heart failure. Heart, 93: 903-907.

Rabie, A., A. El-Kaiaty, M. Hassan and F. Stino, 2018. Influence of some Honey Bee products and a growth promoter supplementation on productive and physiological performance of broiler chickens. Egypt. Poult. Sci. J., 38: 513-531.

Sadarman,, A. Irawan, C. Hidayat, Elfawati, M.M. Sholikin and A. Jayanegara et al, 2021. Propolis supplementation on broiler chicken performances, nutrient digestibility, and carcass characteristics: A meta-analysis. Trop. Anim. Sci. J., 44: 425-433.

He, T., S. Long, S. Mahfuz, D. Wu, X. Wang and X. Piao et al, 2019. Effects of probiotics as antibiotics substitutes on growth performance, serum biochemical parameters, intestinal morphology, and barrier function of broilers. Anim. s, Vol. 9. 10.3390/ani9110985

Heiss, V.A.R.D.C., M.F.D.C. Burbarelli, B.B. Przybulinski, L.C. Garcia, J.R.R.F. Vieira and C.M. Komiyama et al, 2025. Polyphenol blend enhances zootechnical performance, improves meat quality, and reduces the severity of wooden breast in broiler chickens. Front. Vet. Sci., Vol. 12. 10.3389/fvets.2025.1584897

Hashemipour, H., H. Kermanshahi, A. Golian and T. Veldkamp, 2013. Effect of thymol and carvacrol feed supplementation on performance, antioxidant enzyme activities, fatty acid composition, digestive enzyme activities, and immune response in broiler chickens. Poult. Sci., Vol. 92. 10.3382/ps.2012-02685

Ronquillo, M.G. and J.C.A. Hernandez, 2017. Antibiotic and synthetic growth promoters in animal diets: Review of impact and analytical methods. Food Control, Vol. 72. 10.1016/j.foodcont.2016.03.001

Terlouw, E.M.C., B. Picard, V. Deiss, C. Berri, J.-F. Hocquette and M. Gagaoua et al, 2021. Understanding the determination of meat quality using biochemical characteristics of the muscle: Stress at slaughter and other missing keys. Foods, Vol. 10. 10.3390/foods10010084

Idoko, A.S., A. Zaharaddeen, N.U. Imam, S. Nura, B. Abdulazeez and H.A. Olaiya et al, 2020. Physicochemical assessment of broiler chickens fed diets supplemented with a mixture of ginger, garlic and cinnamon. J. Appl. Sci. Environ. Manage., 24: 809-814.

Bowker, B. and H. Zhuang, 2015. Relationship between water-holding capacity and protein denaturation in broiler breast meat. Poult. Sci., Vol. 94. 10.3382/ps/pev120

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Published

2026-05-22

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Section

Research Article

How to Cite

Azza, G. Z., Lyimo, C. M., Luziga, C. D. ., Mdegela, R. H., & Chenyambuga , S. W. (2026). Effects of Propolis as an Organic Growth Promoter on Growth Performance and Carcass Characteristics of Cobb 500 Broiler Chickens. International Journal of Poultry Science, 25, 78–86. https://doi.org/10.66920/ijps.2026.78.86