Comparing the Efficacy of Varieties, Low and High Phytase-expressing Corn, at Equivalent Phytase Levels on Broiler Performance and Bone Ash
DOI:
https://doi.org/10.3923/ijps.2023.192.197Keywords:
Broiler, corn expressed enzyme, phytase, poultry diet, productionAbstract
Objective: A study was conducted to compare the efficacy of two varieties of corn-expressed phytase [low specific activity (203) and high specific activity (1203)] at equivalent FTU kg–1 in broilers. Materials and Methods: Day-old Cobb 500 male broiler chicks were randomly allocated to one of 10 dietary treatments: Positive Control (PC, standard Ca and aP); Negative Control (NC, -0.15% Ca and aP), NC +0.5 kg MT–1 203, NC+1.0 kg MT–1 203, NC +2.0 kg MT–1 203, NC +3.0 kg MT–1 203, T7, NC+0.18 kg MT–1 1203, NC+0.36 kg MT–1 1203, NC+0.72 kg MT–1 1203, NC +1.08 kg MT–1 1203. All birds were weighed on day 14, 28 and 42 to obtain performance parameters: Body weight (BW), feed consumption (FC) and feed conversion ratio (FCR). On d28, left tibias were sampled from 3 birds per pen, for a total of 30 birds per treatment to determine tibia bone ash. Results: On day 14 and d28, birds fed diet supplemented with 203 (day 14, 0.50±0.00 kg, day 28, 1.80±0.00 kg) and 1203 (day 14, 0.50±0.01 kg, day 28, 1.81±0.01 kg) were heavier (p<0.05) than PC (day 14, 0.41±0.00 kg, day 28, 1.65±0.03 kg) and NC (day 14, 0.41±0.00 kg, day 28, 1.50±0.03 kg). On day 42, birds fed diet supplemented with 203 (3.41±0.04 kg) and 1203 (3.45±0.03 kg) were heavier (p<0.05) than NC (3.09±0.07 kg). On day 0-28 and day 0-42, birds fed diet supplemented with 203 (day 0-28, 1.41±0.00, day 0-42, 1.61±0.01) and 1203 (day 0-28, 1.41±0.00, day 0-42, 1.59±0.01) improved FCR (p<0.05) compared to NC (day 0-28, 1.52±0.01, day 0-42, 1.67±0.01). On day 28, birds fed diet supplemented with 203 and 1203 (51.69±0.08%) improved bone ash (p<0.05) when compared to NC (47.95±0.95%). Conclusion: Both low specific activity and high specific activity corn-expressed phytase varieties improved BW, FCR and bone ash compared to PC. Inclusion of either phytase improved broiler performance and bone mineralization in Ca and aP reduced diets and there were no discernible differences in the efficacy of these two enzymes at the tested loading rates.
References
Taylor, T.G. and J.W. Coleman, 1979. A comparative study of the absorption of calcium and the availability of phytate-phosphorus in the golden hamster (Mesocricetus auratus) and the laboratory rat. Br. J. Nutr., 42: 113-119.
Selle, P.H. and V. Ravindran, 2007. Microbial phytase in poultry nutrition. Anim. Feed Sci. Technol., 135: 1-41.
Tamim, N.M., R. Angel and M. Christman, 2004. Influence of dietary calcium and phytase on phytate phosphorus hydrolysis in broiler chickens. Poult. Sci., 83: 1358-1367.
Broz, J., P. Oldale, A.H. Perrin-Voltz, G. Rychen, J. Schulze and C.S. Nunes, 1994. Effects of supplemental phytase on performance and phosphorus utilisation in broiler chickens fed a low phosphorus diet without addition of inorganic phosphates. Br. Poult. Sci., 35: 273-280.
Denbow, D.M., V. Ravindran, E.T. Kornegay, Z. Yi and R.M. Hulet, 1995. Improving phosphorus availability in soybean meal for broilers by supplemental phytase. Poult. Sci., 74: 1831-1842.
Dersjant-Li, Y. and C. Kwakernaak, 2019. Comparative effects of two phytases versus increasing the inorganic phosphorus content of the diet, on nutrient and amino acid digestibility in boilers. Anim. Feed Sci. Technol., 253: 166-180.
Smith, K.A., C.L. Wyatt and J.T. Lee, 2019. Evaluation of increasing levels of phytase in diets containing variable levels of amino acids on male broiler performance and processing yields. J. Applied Poult. Res., 28: 253-262.
Ligon, J.M., 2018. A phytase feed enzyme produced by Zea mays expressing a phytase gene derived from Escherichia coli K12. Agrivida, Inc. https://www.fda.gov/media/131084/download.
Alfonso, C.J.M., L. Blavi, J.N. Broomhead and H.H. Stein, 2018. 275 comparison between a novel phytase and a commercial phytase on growth performance and bone measurements in diets fed to growing pigs. J. Anim. Sci., 96: 147-148.
Zyła, K., M. Mika, B. Stodolak, A. Wikiera, J. Koreleski and S. Ŝwiątkiewicz, 2004. Towards complete dephosphorylation and total conversion of phytates in poultry feeds. Poult. Sci., 83: 1175-1186.
Gautier, A.E., C.L. Walk and R.N. Dilger, 2018. Effects of a high level of phytase on broiler performance, bone ash, phosphorus utilization, and phytate dephosphorylation to inositol. Poult. Sci., 97: 211-218.
Nyannor, E.K.D., P. Williams, M.R. Bedford and O. Adeola, 2007. Corn expressing an Escherichia coli-derived phytase gene: A proof-of-concept nutritional study in pigs. J. Anim. Sci., 85: 1946-1952.
FASS, 2010. Guide for the Care and Use of Agricultural Animals in Research and Teaching. 3rd Edn., Federation of Animal Science Societies, Champaign, Illinois, ISBN: 9781884706110, Pages: 169.
Guerrand, D.A., 2018. Economics of Food and Feed Enzymes: Status and Perspectives. In: Enzymes in Human and Animal Nutrition, Nunes, C.S. and V. Kumar, (Eds.). Academic Press, London, UK, pp: 487–514.
Cowieson, A.J., P. Wilcock and M.R. Bedford, 2011. Super-dosing effects of phytase in poultry and other monogastrics. World's Poultry Science Journal 67: 225-236.
Krieg, J., W. Siegert, D. Berghaus, J. Bock, D. Feuerstein and M. Rodehutscord, 2020. Phytase supplementation effects on amino acid digestibility depend on the protein source in the diet but are not related to insp6 degradation in broiler chickens. Poult. Sci., 99: 3251-3265.
Wang, J. and W.K. Kim, 2021. Evaluation of a novel corn-expressed phytase on growth performance and bone mineralization in broilers fed different levels of dietary nonphytate phosphorus. J. Appl. Poult. Res., Vol. 30.
Nelson, T.S. and A.C. Walker, 1964. The biological evaluation of phosphorus compounds. Poult. Sci., 43: 94-98.
Broomhead, J.N., P.A Lessard, R.M. Raab and M.B. Lanahan, 2019. Effects of feeding corn-expressed phytase on the live performance, bone characteristics, and phosphorus digestibility of nursery pigs. Anim. Sci., 97: 1254-1261.
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