Impact of Locally Isolated Probiotics on Growth Performances, Haemato-Biochemical Profiles and Cecal Microflora of Broiler
DOI:
https://doi.org/10.3923/ijps.2020.586.595Keywords:
Bifidobacterium thermophilum, cecal microflora, growth performance, haemato-biochemical profile, Lactobacillus salivariusAbstract
Background and Objective: Increasing bacterial resistance to antibiotics has contributed to the urge for the development of alternatives to antibiotics. Accordingly, probiotics have received great attention as an alternative to synthetic antibiotics. Locally isolated probiotics may have beneficial effects on animal health and stand for a better alternative to antibiotics. Therefore, the effects of supplementation of locally isolated Lactobacillus salivarius and Bifidobacterium thermophilum on growth performances, haemato-biochemical profiles and cecal microbial compositions of broilers were studied in this experiment. Materials and Methods: A total of 320 day-old unsexed broiler chicks were allocated to 4 treatment groups each containing 4 replicates of 20 chicks. Groups included basal diet (control); control plus Doxycyclin (AGP); control plus L. salivarius (probiotic-I) and control plus B. thermophilum (probiotic-II). Experimental birds were raised for 28 days. Growth performances, haemato-biochemical profiles and cecal microbial composition were analyzed following standard protocol. Results: Probiotic-I impacted significantly on increased body weight and decreased FCR without significant effect in feed consumption, dressing and survivability rate. Probiotics supplementation resulted in higher liver weight in probiotic-I. Significantly higher Hb, RBC and WBC counts were observed in probiotics supplemented groups compared with AGP and control groups. However, total cholesterol, DLCs, PCV, MCV, MCHC were not affected by probiotics supplementation. Total Coliform and Salmonella counts were significantly reduced and Lactobacilli were significantly increased with probiotics supplementation. Conclusion: Locally isolated L. salivarius could be considered as a good potential probiotic for broiler which could be a feasible alternative to antibiotics in broiler diet at finisher stage.
References
Papatsiros, V.G., P.D. Katsoulos, K.C. Koutoulis, M. Karatzia, A. Dedousi and G. Christodoulopoulos, 2013. Alternatives to Antibiotics for Farm Animals. CAB Rev.: Perspect. Agric. Vet. Sci. Nutr. Nat. Resour., Vol. 8.
Huyghebaert, G., R. Ducatelle and F. van Immerseel, 2011. An update on alternatives to antimicrobial growth promoters for broilers. Vet. J., 187: 182-188.
Islam, K.B.M.S., S. Shiraj-Um-Mahmuda and M. Hazzaz-Bin-Kabir, 2016. Antibiotic usage patterns in selected broiler farms of Bangladesh and their Public Health Implications. J. Public Health Dev. Countries, 2: 276-284.
Marković, R., D. Šefer, M. Krstić and B. Petrujkić, 2009. Effect of different growth promoters on broiler performance and gut morphology. Arch. Med. Vet., 41: 163-169.
Wang, Y. and Q. Gu, 2010. Effect of probiotic on growth performance and digestive enzyme activity of Arbor Acres broilers. Res. Vet. Sci., 89: 163-167.
Patterson, J.A. and K.M. Burkholder, 2003. Application of prebiotics and probiotics in poultry production. Poult. Sci., 82: 627-631.
Awad, W.A., K. Ghareeb, S. Abdel-Raheem and J. Bohm, 2009. Effects of dietary inclusion of probiotic and synbiotic on growth performance, organ weights and intestinal histomorphology of broiler chickens. Poult. Sci., 88: 49-56.
Nayebpor, M., P. Farhomand and A. Hashemi, 2007. Effects of different levels of direct fed microbial (Primalac) on growth performance and humoral immune response in broiler chickens. J. Anim. Vet. Adv., 6: 1308-1313.
Ashayerizadeh, A., N. Dabiri, O. Ashayerizadeh, K.H. Mirzadeh, H. Roshanfekr and M. Mamooee, 2009. Effect of dietary antibiotic, probiotic and prebiotic as growth promoters, on growth performance, carcass characteristics and hematological indices of broiler chickens. Pak. J. Biol. Sci., 12: 52-57.
Kabir, S.M.L., M.M. Rahman and M.B. Rahman, 2005. Potentiation of probiotics in promoting microbiological meat quality of broilers. J. Bangladesh Soc. Agric. Sci. Technol., 2: 93-96.
Fernandez, M.F., S. Boris and C. Barbes, 2003. Probiotic properties of human lactobacilli strains to be used in the gastrointestinal tract. J. Applied Microbiol., 94: 449-455.
Matur, E. and E. Eraslan, 2012. The Impact of Probiotics on the Gastrointestinal Physiology. In: New Advances in the Basic and Clinical Gastroenterology, Brzozowski, T., (Ed.). IntechOpen, Poland pp: 51-74.
Islam, K.B.M.S., 2014. Screening of lactobacilli and Bifidobacteria from Bangladeshi indigenous poultry for their potential use as probiotics. Proceeding of 3rd International Conference and Exhibition on Probiotics, Functional and Baby Foods, September 23-25, 2014 OMICS Group Conferences 110-110.
Kandler, O. and N. Weiss, 1986. Genus Lactobacillus. In: Bergey's Manual of Systematic Bacteriology, Sneath, P.H.A., J. Halt, N.S. Nair and M.E. Sharpe (Eds.). Williams and Wilkins, Baltimore, USA., pp: 1216.
Reiner, K., 2010. Catalase Test Protocol. American Society for Microbiology.
Chepete, H. J., E. Chimbombi and R. Tsheko, 2005. Production performance and temperature-humidity index of Cobb 500 broilers reared in open-sided naturally ventilated houses in Botswana. Proceedings of the Seventh International Symposium. 18-20 May 2005 American Society of Agricultural and Biological Engineers.
NRC., 1994. Nutrient Requirements of Poultry. 9th Edn., National Academy Press, Washington, DC., USA., ISBN-13: 9780309048927, Pages: 176.
Kececi, T., H. Oguz, V. Kurtoglu and O. Demet, 1998. Effects of polyvinylpolypyrrolidone, synthetic zeolite and bentonite on serum biochemical and haematological characters of broiler chickens during aflatoxicosis. Br. Poult. Sci., 39: 452-458.
Wu, Y., Y. Zhou, C. Lu, H. Ahmad and H. Zhang et al., 2016. Influence of butyrate loaded clinoptilolite dietary supplementation on growth performance, development of intestine and antioxidant capacity in broiler chickens. PLoS ONE, Vol. 11.
Blajman, J.E., C.A. Olivero, M.L. Fusari, J.A. Zimmermann and E. Rossler et al., 2017. Impact of lyophilized Lactobacillus salivarius DSPV 001P administration on growth performance, microbial translocation, and gastrointestinal microbiota of broilers reared under low ambient temperature. Res. Vet. Sci., 114: 388-394.
Shokryazdan, P., M.F. Jahromi, J.B. Liang, K. Ramasamy, C.C. Sieo and Y.W. Ho, 2017. Effects of a Lactobacillus salivarius mixture on performance, intestinal health and serum lipids of broiler chickens. PLoS ONE, Vol. 12.
Zarei, M., M. Ehsani and M. Torki, 2011. Dietary inclusion of probiotics, prebiotics and synbiotic and evaluating performance of laying hens. Am. J. Agric. Biol. Sci., 6: 249-255.
Lee, K.W., S.H. Lee, H.S. Lillehoj, G.X. Li and S.I. Jang et al., 2010. Effects of direct-fed microbials on growth performance, gut morphometry and immune characteristics in broiler chickens. Poult. Sci., 89: 203-216.
Jung, S.J., R. Houde, B. Baurhoo, X. Zhao and B.H. Lee, 2008. Effects of galacto-oligosaccharides and a Bifidobacteria lactis-based probiotic strain on the growth performance and fecal microflora of broiler chickens. Poult. Sci., 87: 1694-1699.
Mookiah, S., C.C. Sieo, K. Ramasamy, N. Abdullah and Y.W. Ho, 2014. Effects of dietary prebiotics, probiotic and synbiotics on performance, caecal bacterial populations and caecal fermentation concentrations of broiler chickens. J. Sci. Food Agric., 94: 341-348.
Zulkifli, I., N. Abdullah, N.M. Azrin and Y.W. Ho, 2000. Growth performance and immune response of two commercial broiler strains fed diets containing Lactobacillus cultures and oxytetracycline under heat stress conditions. Br. Poult. Sci., 41: 593-597.
Nahashon, S.N., H.S. Nakaue and L.W. Mirosh, 1996. Performance of single comb white leghorn fed a diet supplemented with a live microbial during the growth and egg laying phases. Anim. Feed. Sci. Technol., 57: 25-38.
Ferket, P.R. and A.G. Gernat, 2006. Factors that affect feed intake of meat birds: A review. Int. J. Poult. Sci., 5: 905-911.
Mountzouris, K.C., P. Tsirtsikos, E. Kalamara, S. Nitsch, G. Schatzmayr and K. Fegeros, 2007. Evaluation of the efficacy of a probiotic containing Lactobacillus, Bifidobacterium, Enterococcus and Pediococcus strains in promoting broiler performance and modulating cecal microflora composition and metabolic activities. Poult. Sci., 86: 309-317.
Olnood, C.G., S.S.M. Beski, M. Choct and P.A. Ijia, 2015. Novel probiotics: Their effects on growth performance, gut development, microbial community and activity of broiler chickens. Anim. Nutr., 1: 184-191.
Yeo, J. and K.I. Kim, 1997. Effect of feeding diets containing an antibiotic, a probiotic, or yucca extract on growth and intestinal urease activity in broiler chicks. Poult. Sci., 76: 381-385.
Gaggia, F., P. Mattarelli and B. Biavati, 2010. Probiotics and prebiotics in animal feeding for safe food production. Int. J. Food. Microbiol., 141: S15-S28.
Swiatkiewicz, S., A. Arczewska-Wlosek and D. Jozefiak, 2014. Feed enzymes, probiotic, or chitosan can improve the nutritional efficacy of broiler chicken diets containing a high level of distillers dried grains with solubles. Livestock Sci., 163: 110-119.
O'Dea, E.E., G.M. Fasenko, G.E. Allison, D.R. Korver, G.W. Tannock and L.L. Guan, 2006. Investigating the effects of commercial probiotics on broiler chick quality and production efficiency. Poult. Sci., 85: 1855-1863.
Hatab, M.H., M.A. Elsayed and N.S. Ibrahim, 2016. Effect of some biological supplementation on productive performance, physiological and immunological response of layer chicks. J. Radiat. Res. Applied Sci., 9: 185-192.
Deraz, S.F., A.E. Elkomy and A.A. Khalil, 2019. Assessment of probiotic-supplementation on growth performance, lipid peroxidation, antioxidant capacity and cecal microflora in broiler chickens. J. Applied Pharm. Sci., 9: 30-39.
Sarangi, N.R., L.K. Babu, A. Kumar, C.R. Pradhan, P.K. Pati and J.P. Mishra, 2016. Effect of dietary supplementation of prebiotic, probiotic, and synbiotic on growth performance and carcass characteristics of broiler chickens. Vet. World 9: 313-319.
Haščík, P., L. Trenbecká, M. Bobko, M. Kačániová, J. Čuboň, S. Kunová and O. Bučko, 2016. Effect of diet supplemented with propolis extract and probiotic additives on performance, carcass characteristics and meat composition of broiler chickens. Slovak J. Food Sci., 10: 223-231.
Siadati, S.A., Y. Ebrahimnezhad, G.S. Jouzani and J. Shayegh, 2017. evaluation of probiotic potential of some native lactobacillus strains on the growth performance and serum biochemical parameters of Japanese quails (Coturnix Coturnix Japonica) during rearing period. Braz. J. Poult. Sci., Vol. 19, No. 3.
De Smet, I., L. van Hoorde, N. De Saeyer, M. Vande Woestyne and W. Verstraete, 1994. In vitro study of Bile Salt Hydrolase (BSH) activity of BSH isogenic Lactobacillus plantarum 80 strains and estimation of cholesterol lowering through enhanced BSH activity. Microb. Ecol. Health Dis., 7: 315-329.
Jha, R., R. Das, S. Oak and P. Mishra, 2020. Probiotics (direct-fed microbials) in poultry nutrition and their effects on nutrient utilization, growth and laying performance, and gut health: A systematic review. Animals, Vol. 10.
Beski, S.S.M. and S.Y.T. Al-Sardary, 2015. Effects of dietary supplementation of probiotic and synbiotic on broiler chickens hematology and intestinal integrity. Int. J. Poult. Sci., 14: 31-36.
Kander, M., 2004. Effect of Bifidobacterium sp. on the health state of piglets, determined on the basis of hematological and biochemical indices. Elect. J. Polish Agric. Univ., Vol. 7, No. 2.
Fathi, M., 2013. Effects of Lactobacillus cultures as probiotic on blood parameters, plasma enzymes activities and mortality in broiler chicken. Res. J. Anim. Sci., 7: 77-81.
Kabir, S.M.L., 2009. The role of probiotics in the poultry industry. Int. J. Mol. Sci., 10: 3531-3546.
Cetin, N., B.K. Guclu and E. Cetin, 2005. The effects of probiotic and mannanoligosaccharide on some haematological and immunological parameters in turkeys. J. Vet. Med. Ser. A, 52: 263-267.
Nyamagonda, H., M.N. Swamy, T. Veena, H.D.N. Swamy and K. Jayakumar, 2009. Effect of prebiotic, probiotic and G-probiotic SPL® on certain haematological parameters in broiler chickens. Vet. World, 2: 344-346.
Grindem, C.B., 2011. Schalm's veterinary hematology, 6th edition. Editors: Douglas J. Weiss, K. Jane Wardrop. Vet. Clin. Pathol., 40: 270-270.
Zhang, Z.F. and I.H. Kim, 2013. Effects of probiotic supplementation in different energy and nutrient density diets on performance, egg quality, excreta microflora, excreta noxious gas emission, and serum cholesterol concentrations in laying hens. J. Anim. Sci., 91: 4781-4787.
Vicente, J.L., A. Torres-Rodriguez, S.E. Higgins, C. Pixley, G. Tellez, A.M. Donoghue and B.M. Hargis, 2008. Effect of a selected Lactobacillus spp.-based probiotic on Salmonella enterica serovar enteritidis-infected broiler chicks. Avian Dis., 52: 143-146.
Mountzouris, K.C., P. Tsitrsikos, I. Palamidi, A. Arvaniti, M. Mohnl, G. Schatzmayr and K. Fegeros, 2010. Effects of probiotic inclusion levels in broiler nutrition on growth performance, nutrient digestibility, plasma immunoglobulins and cecal microflora composition. Poult. Sci., 89: 58-67.
Estrada, A., D.C. Wilkie and M. Drew, 2001. Administration of Bifidobacterium bifidum to chicken broilers reduces the number of carcass condemnations for cellulitis at the abattoir. J. Applied Poult. Res., 10: 329-334.
Higgins, S.E., G.F. Erf, J.P. Higgins, S.N. Henderson, A.D. Wolfenden, G. Gaona-Ramirez and B.M. Hargis, 2007. Effect of probiotic treatment in broiler chicks on intestinal macrophage numbers and phagocytosis of Salmonella enteritidis by abdominal exudate cells. Poult. Sci., 86: 2315-2321.
Jin, L.Z., Y.W. Ho, N. Abdullah, M.A. Ali and S. Jalaludin, 1998. Effects of adherent Lactobacillus cultures on growth, weight of organs and intestinal microflora and volatile fatty acids in broilers. Anim. Feed Sci. Technol., 70: 197-209.
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