Effect of Aqueous Extract of Moringa oleifera Leaves on Hematological and Biochemical Parameters in Broilers (Cobb 500)


Authors

  • Ousmane, Issa Abdel Djalil Faculty of Exact and Applied Science, University of Moundou, P.O. Box 206, Moundou, Chad https://orcid.org/0009-0006-3096-4486
  • Mouchili, Mama Department of Biological Sciences Applied to Agriculture, Faculty of Sciences, University of Ebolowa, Cameroon https://orcid.org/0000-0003-3804-866X
  • Oumar, Moucthar Kodbe Biology Laboratory of Faculty of Exact and Applied Sciences, University of N’Djamena, P.O. Box 1117, N’Djamena, Chad https://orcid.org/0009-0009-4269-4454
  • Choupou, Taling Princesse Aicha Animal Production and Nutrition Research Unit, Department of Animal Production, Faculty of Agronomy and Agricultural Sciences, University of Dschang, P.O. Box 222, Dschang, Cameroon https://orcid.org/0009-0007-2669-9517
  • Miegoue, Emile Animal Production and Nutrition Research Unit, Department of Animal Production, Faculty of Agronomy and Agricultural Sciences, University of Dschang, P.O. Box 222, Dschang, Cameroon https://orcid.org/0000-0002-2886-3321
  • Lemoufouet, Jules Animal Production and Nutrition Research Unit, Department of Animal Production, Faculty of Agronomy and Agricultural Sciences, University of Dschang, P.O. Box 222, Dschang, Cameroon https://orcid.org/0000-0003-3544-0658
  • Tendonkeng, Fernand Animal Production and Nutrition Research Unit, Department of Animal Production, Faculty of Agronomy and Agricultural Sciences, University of Dschang, P.O. Box 222, Dschang, Cameroon https://orcid.org/0000-0001-7452-8076

DOI:

https://doi.org/10.3923/ijps.2001.46.52

Keywords:

Biochemical parameters, broiler chicken, hematological parameters, leaf meal, Moringa oleifera

Abstract

Objective: This study was designed to evaluate the effect of the aqueous extract of Moringa oleifera leaves on the hematological and biochemical parameters of broilers. Materials and Methods: The study was conducted at T3F Poultry Farm, Makong from February to April 2024. A total of 150 day-old chicks of Cobb 500 strain, with an average weight of 40.09±3 g, were randomly divided into 20 experimental units according to a completely randomized design with 4 replicates of 7 to 8 subjects each for a period of seven weeks. The experimental rations were formulated such that the aqueous extract of Moringa oleifera leaf was included in the diet at 0 g (R0), 0.25 g (R1), 0.50 g (R2), 0.75 g (R3) and 1 g (R4) both at the beginning and at the end of the study. R0 served as the control ration. Results: The main results showed that the addition of Moringa oleifera leaf extracts in the diet had no significant effect on mean platelet volume levels. On the other hand, mean corpuscular volume, hematocrit and hemoglobin levels increased with increasing levels of Moringa oleifera leaf extract in the diet, while platelet levels decreased with increasing levels of extract. The HDL cholesterol, triglycerides, ALT and AST decreased with the inclusion of aqueous extract of Moringa oleifera leaves in the diet. The addition of aqueous extracts of Moringa oleifera leaves to the diet had no significant effect on total protein, albumin, globulins, total cholesterol, glucose, creatinine and urea. Conclusion: Meat with fewer bacterial residues was obtained when Moringa oleifera leaf extracts were included in the diet, which is beneficial for health-conscious consumers.

References

FAO, 2000. The State of Food and Agriculture 2000. 1st Edn., Food and Agriculture Organization of the United Nations (FAO), Rome, Italy, ISBN: 92-5-104400-7, Pages: 323.

FAO, 2011. Contribution of Livestock Farming to Food Security. 1st Edn., Food and Agriculture Organization of the United Nations, Rome, Italy, ISBN: 978-92-5-207013-9, Pages: 150.

Boka OM., 2006. Evaluation De L’effet Des Anticoccidiens Ionophores Sur Les Performances Zootechniques Des Poulets De Chair En Elevage Semi-Industriel. PhD Thesis Universite Cheikh Anta Diop de Dakar.

Tchoumboué, J. and A. Téguia, 2004. Bases de l’élevage de lapins. Département de zootechnie, Université de Dschang.

Othmani-Mecif, K. and Y. Benazzoug, 2005. Caracterisation de certains parametres biochimiques plasmatiques et histologiques (tractus genital femelle) chez la population locale de Lapin (Oryctolagus cuniculus) non gestante et au cours de la gestation. Sci Technol., 23: 91-96.

Chardon H. and H. Brugere, 2014. Usages des antibiotiques en élevage et filières viandes. Cahiers Sécurité Sanitaire Santé Animale. Centre d’Information des Viandes.

Butaye, P., A. Cloeckaert and S. Schwarz, 2003. Mobile genes coding for efflux-mediated antimicrobial resistance in Gram-positive and Gram-negative bacteria. Int. J. Antimicrob. Agents 22: 205-210.

Ravva, S. and A. Korn, 2015. Effect of neem (Azadirachta indica) on the survival of Escherichia coli O157:H7 in dairy manure. Int. J. Environ. Res. Public Health, 12: 7794-7803.

Tendonkeng, F., B. Boukila, A. Beguidé and T.E. Pamo, 2008. Essai De Substitution Du Tourteau De Soja Par La Farine De Feuilles De Moringa oleifera Dans La Ration Finition Des Poulets De Chair. Conférence Internationale sur le renforcement de la compétitivité en Aviculture Semi-industrielle en Afrique. May 5-9, 2008 Dakar, Sénégal.

Ogbuewu, I.P., V.M.O. Okoro, I.C. Okoli and M.U. Iloeje, 2011. Evaluation of dried leaf meal of an ethnomedicinal plant-neem-on linear growths and reproductive tract morphometry of rabbit does. Electron. J. Environ. Agric. Food Chem., 10: 2153-2159.

Mba, F.F., E. Temgoua, P.D. Kengne and S.N. Kamhoua, 2019. Vulnérabilité des eaux souterraines à la pollution dans la ville de Dschang, Ouest-Cameroun. Int. J. Biol. Chem. Sci., 13: 39-56.

Pesce, M.A. and S.H. Bodourian, 2020. Enzymatic rate method for measuring cholesterol in serum. Clin. Chem., 22: 2042-2045.

Fossati, P. and L. Prencipe, 1982. Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clin. Chem., 28: 2077-2080.

Gornall, A.G., C.J. Bardawill and M.M. David, 1949. Determination of serum proteins by means of the biuret reaction. J. Biol. Chem., 177: 751-766.

Abdel-Fattah, S.A., M.H. El-Sanhoury, N.M. El-Mednay and F. Abdel-Azeem, 2008. Thyroid activity, some blood constituents, organs morphology and performance of broiler chicks fed supplemental organic acids. Int. J. Poult. Sci., 7: 215-222.

Etim, N.N., M.E. Williams, U. Akpabio and E.E.A. Offiong, 2014. Haematological parameters and factors affecting their values. Agric. Sci., 2: 37-47.

Mouchili, M., O.M. Kodbe, H.W. Mekuiko, E. Miegoue, J. Lemoufouet and F. Tendonkeng, 2024. Effects of supplementation with Acacia albida leaves on haematological and biochemical parameters of Arab sheep on natural range. J. Vet. Res. Adv., 6: 31-35.

Antunović, Z., J. Novoselec, M. Šperanda, M. Vegara, V. Pavić, B. Mioč and M. Djidara, 2011. Changes in biochemical and hematological parameters and metabolic hormones in Tsigai ewes blood in the first third of lactation. Arch. Anim. Breed., 54: 535-545.

Bleyere M.N., S. Kamagate, M. Kone, L.K. Kouakou and D. Sawadogo et al., 2010. Influence in vitro d’un extrait aqueux de Bidens pilosa L. (Astéraceae) sur les cellules sanguines, plante utilisée en médecine traditionnelle pour le traitement de l’hypertension artérielle. Médecine d'Afrique Francophone, 5710: 469-477.

Alam, M.M., A.T.M.F.K. Rakib, M.A.A. Hasan, M.S. Hasan and M.A. Ali, 2015. Effects of neem leave powder as a growth promoter in broilers. Int. J. Nat. Social Sci., 2: 22-26.

Messaadia A., S. Saka, M. Krim, I. Maidi, O. Aouacheri and R. Djafer, 2013. L’effet Protecteur D’un Antioxydant Naturel Contre Le Stress Oxydatif Induit Par Le Nitrate D’ammonium Chez Les Rats. PhD Thesis Année Universitaire,

Asmaa, Q., S. Al-Shamerii, M. Al-Tag, A. Al-Shamerii, Y. Li and B.H. Osman, 2017. Parasitological and biochemical studies on cutaneous leishmaniasis in Shara'b District, Taiz, Yemen. Ann. Clin. Microbiol. Antimicrob., Vol. 16.

Downloads

Published

2025-05-09

Issue

Vol. 24 (2025)

Section

Research Article

License

Copyright (c) 2025 The Author(s)

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

How to Cite

Ousmane, ssa A. D., Mouchili, M., Oumar, M. K., Choupou, T. P. A., Miegoue, E., Lemoufouet, J., & Tendonkeng, F. (2025). Effect of Aqueous Extract of Moringa oleifera Leaves on Hematological and Biochemical Parameters in Broilers (Cobb 500). International Journal of Poultry Science, 24, 46–52. https://doi.org/10.3923/ijps.2001.46.52