The Effect of Fermented Sauropus androgynus Plus Bay Leaf Inclusion on the Hematologic and Lipid Profiles of Female Broiler Chickens
DOI:
https://doi.org/10.3923/ijps.2018.410.417Keywords:
Bay leaves, broiler, fermented Sauropus androgynus, hematologic status, internal organ, lipid profilesAbstract
Background and Objective: The use of antibiotics as a feed additive for poultry has been prohibited in many countries because the antibiotics accumulate in the meat, which may stimulate microbial pathogen resistance. The present study was conducted to evaluate the effect of fermented Sauropus androgynus plus bay leaf inclusion on the hematologic and lipid profiles of female broiler chickens. Materials and Methods: Two hundred and eighty female broilers aged 14 days were divided into 7 treatment groups as follows: 1) the control broiler chickens were fed a diet with a commercial feed additive (contained zinc bacitracin) (P0), 2) broiler chickens were fed a diet with medicinal herb mixture formula 1 at 2.5% (P1), 3) broiler chickens were fed a diet with medicinal herb mixture formula 2 at 2.5% (P2), 4) broiler chickens were fed a diet with medicinal herb mixture formula 3 at 2.5% (P3), 5) broiler chickens were fed a diet with medicinal herb mixture formula 1 at 5% (P4), 6) broilers were fed a diet with medicinal herb mixture formula 2 at 5% (P5) and 7) broilers were fed a diet with medicinal herb mixture formula 3 at 5%. Results: Experimental results showed that the inclusion of fermented Sauropus androgynus plus bay leaves had no effect on thrombocyte levels, erythrocyte sedimentation rate (ESR), WBC, RBC, PCV, MCV, MCH, MCHC, liver and spleen weights, intestine length and toxicity but significantly affected the gizzard; the intestine (p<0.01) and heart weights (p<0.05); triglyceride, cholesterol, HDL (p<0.01) LDL and VLDL (p<0.05) levels; and the LDL/HDL ratio (p<0.01). Conclusion: The inclusion of fermented Sauropus androgynus plus bay leaves improved the lipid profile without modifying the hematologic status in female broiler chickens.
References
Jenkins, D.J.A., C.W.C. Kendall, M. Hamidi, E. Vidgen and D. Faulkner et al., 2005. Effect of antibiotics as cholesterol-lowering agents. Metabolism, 54: 103-112.
Cao, J., K. Li, X. Lu and Y. Zhao, 2004. Effects of florfenicol and chromium (III) on humoral immune response in chicks. Asian-Aust. J. Anim. Sci., 17: 366-370.
Liu, B., W. Li, Y. Chang, W. Dong and L. Ni, 2006. Extraction of berberine from rhizome of Coptis chinensis Franch using supercritical fluid extraction. J. Pharm. Biomed. Anal., 41: 1056-1060.
Simitzis, P.E., S.G. Deligeorgis, J.A. Bizelis, A. Dardamani, I. Theodosiou and K. Feggeros, 2008. Effect of dietary oregano oil supplementation on lamb meat characteristics. Meat Sci., 79: 217-223.
Santoso, U., M. Ishikawa and K. Tanaka, 2000. Effects of fermented chub mackerel extract on lipid metabolism of rats fed a high-cholesterol diet. Asian-Aust. J. Anim. Sci., 13: 516-520.
Atawodi, S.E., J.C. Atawodi, G.A. Idakwo, B. Pfundstein and R. Haubner et al., 2010. Evaluation of the polyphenol content and antioxidant properties of methanol extracts of the leaves, stem and root barks of Moringa oleifera Lam. J. Med. Food, 13: 710-716.
Moyo, B., S. Oyedemi, P.J. Masika and V. Muchenje, 2012. Polyphenolic content and antioxidant properties of Moringa oleifera leaf extracts and enzymatic activity of liver from goats supplemented with Moringa oleifera leaves/sunflower seed cake. Meat Sci., 91: 441-447.
Santoso, U. and Sartini, 2001. Reduction of fat accumulation in broiler chickens by Sauropus androgynus (Katuk) leaf meal supplementation. Asian-Aust. J. Anim. Sci., 14: 346-350.
Santoso, U., J. Setianto and T. Suteky, 2005. Effect of Sauropus androgynus (Katuk) extract on egg production and lipid metabolism in layers. Asian-Aust. J. Anim. Sci., 18: 364-369.
Santoso, U. and Y. Fenita, 2016. The effect of Sauropus androgynus leaf extract on performance, egg quality and chemical composition of eggs. J. Indones. Trop. Anim. Agric., 41: 125-134.
Santoso, U., Y. Fenita and E. Sulistyowati, 2017. Effect of Sauropus androgynus leaf extract, fish oil and vitamin E on performance, egg quality and composition in laying hens. J. Indones. Trop. Anim. Agric., 42: 88-98.
Santoso, U., Y. Fenita and K. Kususiyah, 2015. Effect of fermented Sauropus androgynus leaves on blood lipid fraction and haematological profile in broiler chickens. J. lndonesian Trop. Anim. Agric., 40: 199-207.
Samad, A.P.A., U. Santoso, M.C. Lee and F.H. Nan, 2014. Effects of dietary katuk (Sauropus androgynus L. Merr.) on growth, non-specific immune and diseases resistance against Vibrio alginolyticus infection in grouper Epinephelus coioides. Fish Shellfish Immunol., 36: 582-589.
Santoso, U., Y. Fenita and Kususiyah, 2017. The effect of medicinal herb inclusion on hematologic status and blood lipid profiles in broiler chickens. Int. J. Poult. Sci., 16: 415-423.
Andarwulan, N., R. Batari, D.A. Sandrasari, B. Bolling and H. Wijaya, 2010. Flavonoid content and antioxidant activity of vegetables from Indonesia. Food Chem., 121: 1231-1235.
Ayoub, N.A., A.N. Hashim, S.A. Hussein, N.M. Hegazi, H.M. Hassanein and M.A. Nawwar, 2013. Hepatoprotective effect of bay leaves crude extract on primary cultured rat hepatocytes. Eur. Scient. J., 2013: 647-655.
Abu-Dahab, R., V. Kasabri and F.U. Afifi, 2014. Evaluation of the volatile oil composition and antiproliferative activity of Laurus nobilis L. (Lauraceae) on breast cancer cell line models. Records Nat. Prod., 8: 136-147.
Aljamal, A., 2011. Effects of bay leaves on the patients with diabetes mellitus. Res. J. Med. Plant, 5: 471-476.
Ari, M.M., B.A. Ayanwale, T.Z. Adama and E.A. Olatunji, 2012. Effects of different fermentation methods on the proximate composition, amino acid profile and some antinutritional factors (ANFs) in soyabeans (Glycine max). Ferment. Technol. Bioeng., 2: 6-13.
Ibrahim, S.S., R.A. Habiba, A.A. Shatta and H.E. Embaby, 2002. Effect of soaking, germination, cooking and fermentation on antinutritional factors in cowpeas. Food/Nahrung, 46: 92-95.
Olaniyi, L.O. and S. Mehhizadeh, 2013. Effect of traditional fermentation as a pretreatment to decrease the antinutritional properties of rambutan seed (Nephelium lappaceum L.). Proceedings of the International Conference on Food and Agricultural Sciences, October 5-6, 2013, Melaka, Malaysia.
Olagunju, A.I. and B.O.T. Ifesan, 2013. Changes in nutrient and antinutritional contents of sesame seeds during fermentation. J. Microbiol. Biotechnol. Food Sci., 2: 2407-2410.
Su, J.S., B.N. Liu, P.F. Tian, Q. Lin, Y.X. Zhao and X.Z. Ge, 2010. Effect of microbial fermentation on the extraction of alkaloids from radix aconiti and aconite. J. Beijing Univ. Chem. Technol. (Nat. Sci. Edn.), 37: 97-101.
Sukrayana, Y., U. Atmomarsono, V.D. Yunianto and E. Supriyatna, 2011. Improvement of crude protein and crude fiber digestibility of fermented product of palm kernel cake and rice bran mixture for broiler. J. Ilmu Teknologi Peternakan, 1: 167-172.
Santosol, U., S. Ishikawa and K. Tanaka, 2001. Effect of fermented chub mackerel extract on lipid metabolism fed diets without cholesterol. Asian-Austr. J. Anim. Sci., 14: 535-539.
Santoso, U., K. Tanaka, S. Ohtani and M. Sakaida, 2001. Effect of fermented product from Bacillus subtilis on feed conversion efficiency, lipid accumulation and ammonia production in broiler chicks. Asian-Australas. J. Anim. Sci., 14: 333-337.
Santoso, U., S. Ishikawa and K. Tanaka, 2010. Effect of fermented chub mackerel extract on lipid metabolism of diabetic rats. J. Indones. Trop. Anim. Agric., 35: 158-164.
Santoso, U., Y. Fenita, Kususiyah and I.G.N.G. Bidura, 2015. Effect of fermented Sauropus androgynus leaves on meat composition, amino acid and fatty acid compositions in broiler chickens. Pak. J. Nutr., 14: 799-807.
Narahari, D., P. Michealraja, A. Kirubakaran and T. Sujatha, 2005. Antioxidant, cholesterol reducing, immunomodulating and other health promoting properties of herbal enriched designer eggs. Proceedings of the 11th European Symposium on the Quality of Eggs and Egg Products, May 23-26, 2005, Doorwerth, The Netherlands.
Coles, E.H., 1986. Veterinary Clinical Pathology. 4th Edn., W.B. Saunders Co., Philadelphia, London, ISBN: 978-0721618289, Pages: 486.
Schalm, O.W., N.C. Jain and E.J. Carroll, 1975. Veterinary Haemotology. 3rd Edn., Lea and Febiger, Philadelphia.
Brown, B.A., 1976. Direct Methods for Platelet Counts, Rees and Ecker Method. In: Hematology: Principles and Procedures, Brown, B.A. (Ed.). 2nd Edn., Lea and Febiger, Philadelphia, USA., ISBN-13: 978-0812105339, pp: 101-103.
Kulkarni, K.R., 2006. Cholesterol profile measurement by vertical auto profile method. Clin. Lab. Med., 26: 787-802.
Fossati, P. and L. Prencipe, 1982. Serum triglycerides determined colorimetrically with an enzyme that produces hydrogen peroxide. Clin. Chem., 28: 2077-2080.
Brata, B., Y. Fenita, Nurmeiliasari and T. Yogaswara, 2015. Blood profile of layers in response to Sauropus androgynus leaves extract supplementation in fermented palm oil sludge based diet. Proceedings of the International Seminar on Promoting Local Resources for Food and Health, October 12-13, 2015, Bengkulu, Indonesia.
Adnyana, I.K., A. Rosmadi, J.I. Sigit and S.F. Rahmawati, 2012. The effect of Sauropus androgynus leaf juice, sweet potato leaf juice, and kefir on hematology profile of aluminum sulfate-induced anemia mice. Acta Pharm. Indones., 37: 54-58.
Attia, Y.A., A.E. Abd Al-Hamid, M.S. Ibrahim, M.A. Al-Harthi, F. Bovera and A. El-Naggar, 2014. Productive performance, biochemical and hematological traits of broiler chickens supplemented with propolis, bee pollen and mannan oligosaccharides continuously or intermittently. Livest. Sci., 164: 87-95.
Beski, S.S.M. and S.Y.T. Al-Sardary, 2015. Effects of dietary supplementation of probiotic and synbiotic on broiler chickens hematology and intestinal integrity. Int. J. Poult. Sci., 14: 31-36.
Bounous, D.J. and N.L. Stedman, 2000. Normal Avian Hematology: Chicken and Turkey. In: Schalm's Veterinary Hematology, Feldman, B.F., J.G. Zinkl and N.C. Jain (Eds.), John Wiley, Hoboken, New Jersey, ISBN: 9780683306927, pp: 1147-1154.
Elsayed, M., A. Elkomy, M. Aboubakr and M. Morad, 2014. Tissue residues, hematological and biochemical effects of tilmicosin in broiler chicken. Vet. Med. Int., Vol. 2014.
Mushtaq, M., F.R. Durrani, N. Imtiaz, U. Sadique, A. Hafeez, S. Akhtar and S. Ahmad, 2012. Effect of administration of Withania somnifera on some hematological and immunological profile of broiler chicks. Pak Vet. J., 32: 70-72.
Fenita, Y., U. Santoso, Kususiyah, J. Supriyadi, N. Sari and N. Adiyan, 2017. The effect of medicinal herb inclusion on performance, carcass quality and organoleptic profiles in broiler chickens. Proceedings of the National Seminar on the Management, Development and Utilization of Agricultural and Livestock Genetic Resources to Support Sustainable Food Availability, June 2017, Mulawarman University, Samarinda, Indonesia, (In Indonesian).
Fernandes, B.C.S., M.R.F.B. Martins, A.A. Mendes, E.L. Milbradt and C. Sanfelice et al., 2014. Intestinal integrity and performance of broiler chickens fed a probiotic, a prebiotic, or an organic acid. Braz. J. Poult. Sci., 16: 417-424.
Hu, Y., Y. Wang, A. Li, Z. Wang and X. Zhang et al., 2016. Effects of fermented rapeseed meal on antioxidant functions, serum biochemical parameters and intestinal morphology in broilers. Food Agric. Immunol., 27: 182-193.
Kamalia, A. Mujenisa and A. Natsir, 2014. [The Effect of addition different levels of katuk leaf meal in the ration on cholesterol, triglyceride, LDL and HDL of broiler blood]. Buletin Nutrisi Makanan Ternak, 10: 12-18.
Al-Samarrai, O.R., N.A. Naji and R.R. Hameed, 2017. Effect of bay leaf (Laurus nobilis L.) and its isolated (flavonoids and glycosides) on the lipids profile in the local Iraqi female rabbits. Tikrit J. Pure Sci., 22: 72-75.
Belawa, T.G., Yadnya and I.W. Wirawan, 2014. The effect of corn meal substituted with sengauk in diets contain starpig supplemented by Syzygium polyanthum on feed efficiency and blood lipid profile of Kampoeng chicken. Majalah Ilmiah Peternakan, 17: 95-99.
Khan, A., G. Zaman and R.A. Anderson, 2009. Bay leaves improve glucose and lipid profile of people with type 2 diabetes. J. Clin. Biochem. Nutr., 44: 52-56.
Aljamal, A., 2010. Effects of bay leaves on blood glucose and lipid profiles on the patients with type 1 diabetes. World Acad. Sci. Eng. Technol., 69: 211-214.
Sutrisna, E., Y. Nuswantoro and R.F. Said, 2018. Hypolipidemic of ethanolic extract of Salam bark (Syzygium polyanthum (Wight) Walp.) from Indonesia (Preclinical study). Drug Invent. Today, 10: 55-58.
Verawati, D. Nofiandi and Petmawati, 2017. Effect of extraction method on total phenolic content and antioxidant activity of bay leaf (Syzygium polyanthum (Wight) Walp.). J. Katalisator, 2: 53-60.
Frankel, E.N., J.B. German, J.E. Kinsella, E. Parks and J. Kanner, 1993. Inhibition of oxidation of human low-density lipoprotein by phenolic substances in red wine. Lancet, 341: 454-457.
Kerry, N.L. and M. Abbey, 1997. Red wine and fractionated phenolic compounds prepared from red wine inhibit low density lipoprotein oxidation in vitro. Artherosclerosis, 135: 93-102.
Reed, J., 2002. Cranberry flavonoids, atherosclerosis and cardiovascular health. Crit. Rev. Food Sci. Nutr., 42: 301-316.
Bentz, A.B., 2009. A review of quercetin: chemistry, antioxidant properties and bioavailability. J. Young Investigators, 9: 120-128.
Tebib, K., P. Besancon and J.M. Rouanet, 1994. Dietary grape seed tannins affect lipoproteins, lipoprotein lipases and tissue lipids in rats fed hypercholesterolemic diets. J. Nutr., 124: 2451-2457.
Afrose, S., M.S. Hossain, T. Maki and H. Tsujii, 2010. Hypocholesterolemic response to Karaya saponin and Rhodobacter capsulatus in broiler chickens. Asian-Aust. J. Anim. Sci., 23: 733-741.
Elekofehinti, O.O., J.P. Kamdem, I.J. Kade, J.B.T. Rocha and I.G. Adanlawo, 2013. Hypoglycemic, antiperoxidative and antihyperlipidemic effects of saponins from Solanum anguivi Lam. fruits in alloxan-induced diabetic rats. South Afr. J. Bot., 88: 56-61.
Khan, N., M.S. Akhtar, B.A. Khan, V.D.A. Braga and A. Reich, 2015. Antiobesity, hypolipidemic, antioxidant and hepatoprotective effects of Achyranthes aspera seed saponins in high cholesterol fed albino rats. Arch. Med. Sci., 11: 1261-1271.
Xu, B.J, L.L. Han, Y.N. Zheng, J.H. Lee and C.K. Sung, 2002. In vitro inhibitory effect of triterpenoidal saponins from platycodi radix on pancreatic lipase. Arch. Pharm. Res., 28: 180-185.
Kimura, H., S. Ogawa, M. Jisaka, Y. Kimura, T. Katsube and K. Yokota, 2006. Identification of novel saponins from edible seeds of Japanese horse chestnut (Aesculus turbinata blume) after treatment with wooden ashes and their nutraceutical activity. J. Pharm. Biomed. Anal., 41: 1657-1665.
Wang, T., R.C. Choi, J. Li, C.W. Bi and W. Ran et al., 2012. Trillin, a steroidal saponin isolated from the rhizomes of Dioscorea nipponica, exerts protective effects against hyperlipidemia and oxidative stress. J. Ethnopharmacol., 139: 214-220.
Nofer, J.R., B. Kehrel, M. Fobker, B. Levkau, G. Assmann and A. von Eckardstein, 2002. HDL and arteriosclerosis: Beyond reverse cholesterol transport. Atherosclerosis, 161: 1-16.
Downloads
Published
Issue
Section
License
Copyright (c) 2018 The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.