Effects of Amino Acid Composition in a Bacillus amyloliquefaciens-fermented Mixture of Bovine Blood and Coconut Pulp on Growth Performance, Blood Cholesterol of Broilers
DOI:
https://doi.org/10.3923/ijps.2017.274.280Keywords:
Bacillus amyloliquefaciens, bovine blood, broiler, coconut pulp, fermentationAbstract
Background and Background: Bovine blood is a livestock by-product that can be used as a protein source for livestock, particularly when incorporated in broiler rations. This study was conducted to investigate the effects of amino acids in Bacillus amyloliquefaciens-fermented mixtures of bovine blood and coconut pulp (blood meal) on growth performance, blood cholesterol and erythrocyte content in broilers. Methodology: A total of 100 six day-old CP 707 (Strain Cobb) broilers were divided into 5 experimental groups with 4 replicates of 5 broilers. The experimental groups received rations supplemented with 0, 5, 10, 15 and 20% blood meal that substituted for fish meal or soybean meal. The experimental period was 5 weeks. Treatment effects on broiler performance parameters such as final body weight, final weight gain, average daily gain, feed intake, average abdominal fat and Feed Conversion Ratio (FCR), total serum cholesterol and erythrocyte count, as well as the return on investment were determined. Results: The group fed rations supplemented with 10% blood meal showed final body weight, final weight gain and FCR of 1,172, 1,035 and 1.75 g, respectively, which was significantly higher (p<0.05) than that for the other treatments. Rations with 10% blood meal also had the best return on investment ($0.92) relative to the other groups. Conclusion: A mixture of 10% bovine blood and coconut pulp fermented with Bacillus amyloliquefaciens can be used in broiler feed without adversely affecting broiler performance and can replace 47% of total fishmeal and 53% of soybean meal in broiler rations.
References
DGAHAH., 2016. Population and livestock production in Indonesia. Directorate General of Animal Husbandry and Animal Health, Indonesia.
CBS., 2015. The number of cattle slaughtering in 2013. Central Bureau of Statistics, Indonesia.
Rasyaf, M., 1994. Maintaining Quail. Kanisius, Yogyakarta, Indonesia.
Donkoh, A., C.C. Atuahene, D.M. Anang and S.K. Ofori, 1999. Chemical composition of solar-dried blood meal and its effect on performance of broiler chickens. Anim. Feed. Sci. Technol., 81: 299-307.
Scott, M.L., M.C. Neisheim and R.J Young, 1982. Nutrion of the Chicken. 3rd Edn., M.L. Scott and Associates, Ithaca, New York.
Hidayati, S.G., 2011. Coconut pulp processing with local microbes as alternative feed ingredients for poultry in West Sumatra. J. Embrio, 4: 26-36.
Khawaja, T., S.H. Khan and N.N. Ansari, 2007. Effect of different levels of blood meal on broiler performance during two phases of growth. Int. J. Poult. Sci., 6: 860-865.
Ekunseitan, D.A., O.O. Balogun, O.M. Sogunle, A.O. Yusuf and A.A. Ayoola et al., 2013. Health status of birds fed diets containing three differently processed discarded vegetable-bovine blood-rumen content mixtures. Pak. J. Biol. Sci., 16: 325-331.
NRC., 1994. Nutrient Requirement for Poultry. 9th Edn., National Academy Press, Washington DC, USA.
Cieslak, D.G. and N.J. Benevenga, 1984. The effect of amino acid excess on utilization by the rat of the limiting amino acid-threonine. J. Nutr., 114: 1871-1877.
Gietzen, D.W., 1993. Neural mechanisms in the responses to amino acid deficiency. J. Nutr., 123: 610-625.
Moran, C.A., 2001. Development and benefits of liquid diets for newly weaned pigs. Ph.D. Thesis, University of Plymouth, USA.
Madigan, M., J. Martinko, D. Stahl and D. Clark, 2012. Brock Biology of Microorganisms. 13th Rev. Edn., Pearson, New York.
Chibata, I., Toyonaka, M. Kisumi, Higasinada-Ku, Kobe and Y. Ashikaga, 1959. Process for producing alfa-isoleucine. Claims Priority. 8 Claims (Cl. 195-29). U.S. Patent, Washington, USA.
Nollet, L.M.L., 1996. Handbook of Food Analysis. Vol. 1, Marcel Dekker. Inc., New York.
Sigma-Aldrich, 2012. HDL and LDL/VLDL Quantitation Kit: MAK045 SIGMA. Sigma-Aldrich Co. LLC., Germany.
SPSS., 1999. SPSS Base 11.0 for Windows User's Guide. SPSS Inc., Chicago IL., USA.
Onwudike, O.C., 1981. Effect of various protein sources on egg production in a tropical environment. Trop. Anim. Prod., 6: 249-256.
NRC., 1993. Nutrient Requirement of Warm Water Fishes and Shelfish. Nutritional Academy of Sciences, Washington DC., USA., Pages: 102.
Kim, Y.O., J.K. Lee, H.K. Kim, J.H. Yu and T.K. Oh, 1998. Cloning of the thermostable phytase gene (phy) from Bacillus sp. DS11 and its over expression in Escherichia coli. FEMS Microbiol. Lett., 162: 185-191.
Cho, S.J., 2009. Isolation and characterization of mannanase producing Bacillus amyloliquefaciens CS47 from horse feces. J. Live Sci., 19: 1724-1730.
Mabrouk, M.E.M. and A.M.D. El Ahwany, 2008. Production of β-mannanase by Bacillus amylolequifaciens 10A1 cultured on potato peels. Afr. J. Biotechnol., 7: 1123-1128.
Das, M.P., L.J. Rebecca, S. Sharmila, Anu, A. Banerjee and D. Kumar, 2012. Identification and optimization of cultural conditions for chitinase production by Bacillus amyloliquefaciens SM3. J. Chem. Pharma. Res., 4: 4816-4821.
Rahman, A., 1989. Introduction fermentation technology. Inter-University Center for Food and Nutrition, IPB., Bogor.
Musa, H.H., G.H. Chen, J.H. Cheng and G.M. Yousif, 2008. Relation between abdominal fat and serum cholesterol, triglycerides and lipoprotein concentrations in chicken breeds. Turk. J. Vet. Anim. Sci., 31: 375-379.
Kalavathy, R., N. Abdullah, S. Jalaludin and Y.W. Ho, 2003. Effects of Lactobacillus cultures on growth performance, abdominal fat deposition, serum lipids and weight of organs of broiler chickens. Br. Poult. Sci., 44: 139-144.
Moghadam, M.B., M. Rezaei, F. Niknafs and H. Sayyahzadeh, 2009. Effect of Combined Probiotic and Organic Acid on Some Blood Parameters and Immune System of Broiler Chicks. In: Proceedings of the 2nd Mediterranean Summit of World Poultry Science Association, October 4-7, 2009, WPSA. (Ed.)., World Poultry Science Association, The Netherlands, pp: 303-308.
Adebambo, A.O., M.A. Adeleke, M. Whetto, S.O. Peters and C.O.N. Ikeobi et al., 2010. Combining abilities of carcass traits among pure and crossbred meat type chickens. Int. J. Poult. Sci., 9: 777-783.
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