Sequence Analysis of Infectious Bronchitis Virus IS/1494 like Strain Isolated from Broiler Chicken Co-Infected with Newcastle Disease Virus in Egypt During 2012
DOI:
https://doi.org/10.3923/ijps.2014.530.536Keywords:
Broiler chickens, IBV, mixed infection, sequence, variant strainsAbstract
In the present study, we characterized IBV field isolate from a vaccinated broiler flock suspected to be co infected Newcastle disease virus (NDV). The flock demonstrated characteristic clinical signs, post mortem lesions and was positive to IBV and NDV by RT-PCR. The (S1) spike glycoprotein gene of (IBV) isolate was partially amplified by RT-PCR, then directly sequenced. The virus was designated (IBV/chicken/Egypt/VRLCU154/2012). Blast analysis, multiple alignments and phylogenetic analysis revealed that IBV isolate was in close relation to the IBV isolate IS/1494/06 (EU780077.2) with high nucleotide homology (97%). The sequence was submitted to NCBI GenBank with the accession No.JX893950. The results of the present study indicate that IS/1494 like strains might have a similar origin based on the genetic relationship among variants and reference strains.
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