Actigen® Influence on the Gene Expression of Heat Shock Proteins in Ross 708 Broiler Chickens
DOI:
https://doi.org/10.3923/ijps.2014.114.123Keywords:
Actigen®,, chicken, gene expression, heat shock proteinAbstract
In field trials, heat-exposed chickens given Actigen®, a second generation mannan oligosaccharide (MOS) from Saccharomyces cerevisiae, maintained good intestinal health and performance. This investigation explored the influence of Actigen® on heat shock protein (HSP) responses in Ross 708 broiler chickens. Gender-segregated broilers were given either a control or Actigen®-supplemented (800 g/ton in starter, 400 g/ton in grower and 200 g/ton in finisher) diet over a 6 week growing period. At 3 and 6 weeks of age, broilers of each gender on each diet were exposed to 41°C for 1 h in a temperature-controlled chamber while controls were maintained at 24°C. After heat exposure, liver and ileum tissues were collected and preserved in RNAlater for determination of gene expression via Real Time PCR. Significant differences in mRNA expression for HSP90A, HSP90AA and HSP90B due to gender were found in the ileum, but no gender-related differences for these HSPs were found in the liver. In all heat-exposed birds, gene expression was elevated for HSP90A, HSP90AA, HSP90B, HSP70 and HSP60 in both liver and ileum with males at 3 and 6 weeks of age showing the greater HSP response. Lower Actigen®-related HSP90AA and HSP90B mRNA expression in the liver suggested that Actigen® potentially modified HSP expression outside the intestinal tract. Actigen® mechanism (s) of action outside the intestine are equivocal, but they might be indirect. Lower HSP mRNA expression in Actigen®-fed birds indicated that the supplement can modify the HSP response while allowing continued good performance during heat-exposure.
References
Fox, T.W., 1980. The effects of thiouracil and thyroxine on resistance to heat shock. Poult. Sci., 59: 2391-2396.
Gonzalez-Esquerra, R. and S. Leeson, 2005. Effects of acute versus chronic heat stress on broiler response to dietary protein. Poult. Sci., 84: 1562-1569.
Graham, G., P.J. Sharp, Q. Li, P.W. Wilson, R.T. Talbot, A. Downing and T. Boswell, 2009. HSP90B1, a thyroid hormone-responsive heat shock protein gene involved in photoperiodic signaling. Brain Res. Bull., 79: 201-207.
Hendrick, J.P. and F.U. Hartl, 1993. Molecular chaperone functions of heat-shock proteins. Annu. Rev. Biochem., 62: 349-384.
Hightower, L.E., 1991. Heat shock, stress proteins, chaperones and proteotoxicity. Cell, 66: 191-197.
Hooge, D.M. and A. Connolly, 2011. Meta-analysis summary of broiler chicken trials with dietary Actigen® (2009-2011). Int. J. Poult. Sci., 10: 819-824.
Liew, P.K., I. Zulkifli, M. Hair-Bejo, A.R. Omar and D.A. Israf, 2003. Effects of early age feed restriction and heat conditioning on heat shock protein 70 expression, resistance to infectious bursal disease and growth in male broiler chickens subjected to heat stress. Poult. Sci., 82: 1879-1885.
Lin, T.W., C.W. Lo, S.Y. Lai, R.J. Fan and C.J. Lo et al., 2007. Chicken heat shock protein 90 is a component of the putative cellular receptor complex of infectious bursal disease virus. J. Virol., 81: 8730-8741.
Lindquist, S., 1986. The heat shock response. Annu. Rev. Biochem., 55: 1151-1191.
Mahmoud, K., 2000. Genetic and environmental variations of chicken heat shock protein. Ph.D. Thesis, North Carolina State University, Raleigh, North Carolina.
Mahmoud, K.Z. and F.W. Edens, 2003. Influence of selenium sources on age-related and mild heat stress-related changes of blood and liver glutathione redox cycle in broiler chickens (Gallus domesticus). Comp. Biochem. Physiol. Part B: Biochem. Mol. Biol., 136: 921-934.
Mahmoud, K.Z. and F.W. Edens, 2005. Influence of organic selenium on hsp70 response of heat-stressed and enteropathogenic Escherichia coli-challenged broiler chickens (Gallus gallus). Comp. Biochem. Physiol. C: Toxicol. Pharmacol., 141: 69-75.
Mahmoud, K.Z., F.W. Edens, E.J. Eisen and G.B. Havenstein, 2003. Effect of ascorbic acid and acute heat exposure on heat shock protein 70 expression by young white leghorn chickens. Comp. Biochem. Physiol. Part C: Toxicol. Pharmacol., 136: 329-335.
Mahmoud, K.Z., F.W. Edens, E.J. Eisen and G.B. Havenstein, 2004. Ascorbic acid decreases heat shock protein 70 and plasma corticosterone response in broilers (Gallus gallus domesticus) subjected to cyclic heat stress. Comp. Biochem. Physiol. Part B: Biochem. Mol. Biol., 137: 35-42.
Mahmoud, K.Z., F.W. Eden, E.J. Eisen and G.B. Havenstein, 2004. The effect of dietary phosphorus on heat shock protein mRNAs during acute heat stress in male broiler chickens (Gallus gallus). Comp. Biochem. Physiol. Part C: Toxicol. Pharmacol., 137: 11-18.
May, J.D., J.W. Deaton, F.N. Reece and S.L. Branton, 1986. Effect of acclimation and heat stress on thyroid hormone concentration. Poult. Sci., 65: 1211-1213.
McCormick, C.C., J.D. Garlich and F.W. Edens, 1980. Effect of calcium deficiency on survival time of young chickens acutely exposed to high temperature. J. Nutr., 110: 837-850.
Miska, K.B., R.H. Fetterer, W. Min and H.S. Lillehoj, 2005. Heat shock protein 90 genes of two species of poultry Eimeria: Expression and evolutionary analysis. J. Parasitol., 91: 300-306.
Morimoto, R.I., D.A. Jurivich, P.E. Kroeger, S.K. Mathur and S.P. Murphy et al., 1995. Regulation of Heat Shock Gene Transcription by a Family of Heat Shock Factors. In: The Biology of Heat Shock Proteins and Molecular Chaperones, Morimoto, R.I., A. Tissieres and C. Georgopoulos (Eds.). Cold Spring Harbor Laboratory Press, New York, pp: 417-455.
Nathan, D.F., M.H. Vos and S. Lindquist, 1997. In vivo functions of the Saccharomyces cerevisiae Hsp90 chaperone. Proc. Natl. Acad. Sci., 94: 12949-12956.
Niu, Z.Y., F.Z. Liu, Q.L. Yan and W.C. Li, 2009. Effects of different levels of selenium on growth performance and immunocompetence of broilers under heat stress. Arch. Anim. Nutr., 63: 56-65.
Niu, Z.Y., F.Z. Liu, Q.L. Yan and W.C. Li, 2009. Effects of different levels of vitamin E on growth performance and immune responses of broilers under heat stress. Poult. Sci., 88: 2101-2107.
May, J.D., 1982. Effect of dietary thyroid hormone on survival time during heat stress. Poult. Sci., 61: 706-709.
Pardue, S.L., J.P. Thaxton and J. Brake, 1985. Influence of supplemental ascorbic acid on broiler performance following exposure to high environmental temperature. Poult. Sci., 64: 1334-1338.
Pauli, D.A. and A. Tissieres, 1990. Developmental Expression of the Heat Shock Genes in Drosophih melanogaster. In: Stress Protein in Biology and Medicine, Morimoto, R.I., A.C. Tissieres and C. Georpopoulous (Eds.). Cold Springs Harbor Laboratory Press, Cold Springs Harbor, NY., USA., pp: 361-378.
Pratt, W.B. and D.O. Toft, 1997. Steroid receptor interactions with heat shock protein and immunophilin chaperones. Endocrine Rev., 18: 306-360.
Quinteiro-Filho, W.M., A. Ribeiro, V. Ferraz-de-Paula, M.L. Pinheiro and M. Sakai et al., 2010. Heat stress impairs performance parameters, induces intestinal injury and decreases macrophage activity in broiler chickens. Poult. Sci., 89: 1905-1914.
Ritossa, F., 1962. A new puffing pattern induced by temperature shock and DNP in drosophila. Experientia, 18: 571-573.
Roussan, D.A., G.Y. Khwaldeh, R.R. Haddad, I.A. Shaheen, G. Salameh and R. Al Rifai, 2008. Effect of ascorbic acid, acetylsalicylic acid, sodium bicarbonate and potassium chloride supplementation in water on the performance of broiler chickens exposed to heat stress. J. Applied Poult. Res., 17: 141-144.
Rozenboim, I., E. Tako, O. Gal-Garber, J.A. Proudman and Z. Uni, 2007. The effect of heat stress on ovarian function of laying hens. Poult. Sci., 86: 1760-1765.
Schlesinger, M.J., 1990. Heat shock proteins: Minireview. J. Biol. Chem., 265: 12111-12114.
Seifert, S. and B. Watzl, 2007. Inulin and oligofructose: Review of experimental data on immune modulation. J. Nutr., 137: 2563S-2567S.
Sims, M.D., K.A. Dawson, K.E. Newman, P. Spring and D.M. Hoogell, 2004. Effects of dietary mannan oligosaccharide, bacitracin methylene disalicylate, or both on the live performance and intestinal microbiology of turkeys. Poult. Sci., 83: 1148-1154.
Sohail, M.U., Z.U. Rahman, A. Ijaz, M.S. Yousaf and K. Ashraf et al., 2011. Single or combined effects of mannan-oligosaccharides and probiotic supplements on the total oxidants, total antioxidants, enzymatic antioxidants, liver enzymes and serum trace minerals in cyclic heat-stressed broilers. Poult. Sci., 90: 2573-2577.
Soleimani, A.F., I. Zulkifli, A.R. Omar and A.R. Raha, 2011. Physiological responses of 3 chicken breeds to acute heat stress. Poult. Sci., 90: 1435-1440.
Spring, P., C. Wenk, K.A. Dawson and K.E. Newman, 2000. The effects of dietary mannaoligosaccharides on cecal parameters and the concentrations of enteric bacteria in the ceca of salmonella-challenged broiler chicks. Poult. Sci., 79: 205-211.
SAS Institute, 2012. JMP 10 Scripting Guide. SAS Institute, Cary, NC., ISBN-13: 9781612901954, Pages: 828.
Teeter, R.G. and M.O. Smith, 1986. High chronic ambient temperature stress effects on broiler acid-base balance and their response to supplemental ammonium chloride, potassium chloride and potassium carbonate. Poult. Sci., 65: 1777-1781.
Wang, S., 1992. Steroidal modulation of gene expression of heat-shock proteins in domestic chickens. Ph.D. Thesis, The Graduate School, North Carolina State University, Raleigh, NC.
Wang, S. and F.W. Edens, 1994. Hsp70 mRNA expression in heat-stressed chickens. Comp. Biochem. Physiol. Part B: Comparat. Biochem., 107: 33-37.
Wang, S. and F.W. Edens, 1998. Heat conditioning induces heat shock proteins in broiler chickens and turkey poults. Poult Sci., 77: 1636-1645.
Wang, S. and F.W. Edens, 2008. Involvement of steroid hormones, corticosterone and testosterone, in synthesis of heat shock proteins in broiler chickens. Int. J. Poult. Sci., 7: 783-797.
Xiao, R., R.F. Power, D. Mallonee, K. Routt and L. Spangler et al., 2012. Effects of yeast cell wall-derived mannan-oligosaccharides on jejunal gene expression in young broiler chickens. Poult. Sci., 91: 1660-1669.
Yahav, S., S. Goldfeld, I. Plavnik and S. Hurwitz, 1995. Physiological responses of chickens and turkeys to relative humidity during exposure to high ambient temperature. J. Thermal Biol., 20: 245-253.
Yu, J., E. Bao, J. Yan and L. Lei, 2008. Expression and localization of Hsps in the heart and blood vessel of heat-stressed broilers. Cell Stress Chape., 13: 327-335.
Ahmad, T. and M. Sarwar, 2006. Dietary electrolyte balance: Implications in heat stressed broiler. World's Poult. Sci. J., 62: 638-653.
Ahmad, T., T. Khalid, T. Mushtaq, M.A. Mirza, A. Nadeem, M.E. Babar and G. Ahmad, 2008. Effect of potassium chloride supplementation in drinking water on broiler performance under heat stress conditions. Poult. Sci., 87: 1276-1280.
Ait-Boulahsen, A., J.D. Garlich and F.W. Edens, 1993. Calcium deficiency and food deprivation improve the response of chickens to acute heat stress. J. Nutr., 123: 98-105.
Ait-Boulahsen, A., J.D. Garlich and F.W. Edens, 1995. Potassium chloride improves the thermotolerance of chickens exposed to acute heat stress. Poult. Sci., 74: 75-87.
Attia, Y.A., R.A. Hassan and E.M.A. Qota, 2009. Recovery from adverse effects of heat stress on slow-growing chicks in the tropics 1: Effect of ascorbic acid and different levels of betaine. Trop. Anim. Health Prod., 41: 807-818.
Barral, J.M., S.A. Broadley, G. Schaffar and F.U. Hartl, 2004. Roles of molecular chaperones in protein misfolding diseases. Semin. Cell Dev. Biol., 15: 17-29.
Baurhoo, B., L. Phillip and C.A. Ruiz-Feria, 2007. Effects of purified lignin and mannan oligosaccharides on intestinal integrity and microbial populations in the ceca and litter of broiler chickens. Poult. Sci., 86: 1070-1078.
Bollengier-Lee, S., M.A. Mitchell, D.B. Utomo, P.E. Williams and C.C. Whitehead, 1998. Influence of high dietary vitamin E supplementation on egg production and plasma characteristics in hens subjected to heat stress. Br. Poult. Sci., 39: 106-112.
Borrelli, M.J., D.M. Stafford, L.A. Karczewski, C.M. Rausch, Y.J. Lee and P.M. Corry, 1996. Thermotolerance expression in mitotic CHO cells without increased translation of heat shock proteins. J. Cell. Physiol., 169: 420-428.
Bottje, W.G. and P.C. Harrison, 1985. Effects of carbonated water on growth performance of cockerels subjected to constant and cyclic heat stress temperatures. Poult. Sci., 64: 1285-1292.
Bozkurt, M., K. Kucukyilmaz, A.U. Catli, M. Cinar, E. Bintas and F. Coven, 2012. Performance, egg quality and immune response of laying hens fed diets supplemented with mannan-oligosaccharide or an essential oil mixture under moderate and hot environmental conditions. Poult. Sci., 91: 1379-1386.
Bradley, G.L., T.F. Savage and K.I. Timm, 1994. The effects of supplementing diets with Saccharomyces cerevisiae var. boulardii on male poult performance and ileal morphology. Poult. Sci., 73: 1766-1770.
Bukau, B. and A.L. Horwich, 1998. The Hsp70 and Hsp60 chaperone machines. Cell, 92: 351-366.
Cahaner, A., Y. Pinchasov, I. Nir and Z. Nitsan, 1995. Effects of dietary protein under high ambient temperature on body weight, breast meat yield and abdominal fat deposition of broiler stocks differing in growth rate and fatness. Poult. Sci., 74: 968-975.
Collett, S.R., 2005. Strategies for Improving Gut Health in Commercial Broiler Operations. In: Nutritional Biotechnology in the Feed and Food Industries: Proceedings of Alltech's Twentieth Annual Symposium, Lyons, T.P. and K.A. Jacques (Eds.). Nottingham University Press, Thrumpton, Nottingham, UK., pp: 17-29.
Edens, F.W., 1976. Body temperature and blood chemistry responses in broiler cockerels given a single intravenous injection of Na+ or Ca++ before an acute heating episode. Poult. Sci., 55: 2248-2255.
Edens, F.W., 1977. Physiological profile of heat prostration in chickens. Proceedings of the 13th Annual Meeting: Atlanta, Georgia, January 23, 1977, Atlanta, GA., pp: 34-53.
Edens, F.W. and H.S. Siegel, 1975. Adrenal responses in high and low acth response lines of chickens during acute heat stress. Gen. Comp. Endocrinol., 25: 64-73.
Edens, F.W. and H.S. Siegel, 1976. Modification of corticosterone and glucose responses by sympatholytic agents in young chickens during acute heat exposure. Poult. Sci., 55: 1704-1712.
Eshdat, Y., I. Ofek, Y. Yehuidit-Gan, Y. Yashouv-Gan, N. Sharon and D. Mirelmann, 1978. Isolation of a mannose-specific lectin from E. coli and its role in the adherence of the bacteria to epithelial cells. Biochem. Biophys. Res. Commun., 85: 1551-1559.
Fayet, O., T. Ziegelhoffer and C. Georgopoulos, 1989. The groES and groEL heat shock gene products of Escherichia coli are essential for bacterial growth at all temperatures. J. Bacteriol., 171: 1379-1385.
Ferket, P.R. and M.A. Qureshi, 1992. Performance and immunity of heat-stressed broilers fed vitamin- and electrolyte-supplemented drinking water. Poult. Sci., 71: 88-97.
Ferket, P.R., A.A. Santos Jr. and E.O. Oviedo-Rondon, 2005. Dietary factors that affect gut health and pathogen colonization. Proceedings of the 32nd Carolina Poultry Nutrition Conference, October 26-27, 2005, Research Triangle Park, NC., USA., pp: 1-22.
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